GPR177 (WLS) (NM_001193334) Human Tagged ORF Clone

CAT#: RC231242

WLS (Myc-DDK-tagged)-Human wntless homolog (Drosophila) (WLS), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY434241) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231242 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WLS
• Synonyms: C1orf139; EVI; GPR177; mig-14; MRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231242 representing NM_001193334
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGCAATTATAGAAAACATGAGCACCAAGAAGCTGTGCATTGTTGGTGGGATTCTGCTCGTGT
  TCCAAATCATCGCCTTTCTGGTGGGAGGCTTGATTGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCT
  GGCTTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAA
  TGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCA
  TGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAAGAAGAA
  AATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGGCTTCACC
  AAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGA
  GGATCACCATGATGTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGAC
  CTTTATCAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGAC
  ATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGG
  ATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTG
  CCTCTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACA
  GACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGT
  TTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGCTATGAG
  CAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCACCTTGGCC
  TGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCG
  TCACAGTCCAAGTGAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCT
  GATGTTCTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGGCGATCTGGGTGTCCAT
  AGTGGGGAAGAACTCCAGCTCACCACCACTATCACCCATGTGGACGGACCCACTGAGATCTACAAGTTGA
  CCCGCAAGGAGGCCCAGGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231242 representing NM_001193334
  Red=Cloning site Green=Tags(s)
  
  MAGAIIENMSTKKLCIVGGILLVFQIIAFLVGGLIGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLK
  CTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFT
  KVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGD
  IRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTT
  DIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLA
  CAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVH
  SGEELQLTTTITHVDGPTEIYKLTRKEAQE
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193334
• ORF Size: 1350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001193334.1, NP_001180263.1
• RefSeq ORF: 1353
• Locus ID: 79971
• Protein Families: Transmembrane
• MW: 52 kDa