Sohlh2 (CCDC169-SOHLH2) (NM_001198910) Human Tagged ORF Clone

CAT#: RC231278

CCDC169 (Myc-DDK-tagged)-Human C13orf38-SOHLH2 readthrough (C13orf38-SOHLH2)

Product Images

Western blot validation of overexpression lysate (Cat# LY434277) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231278 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CCDC169-SOHLH2 protein (Cat# TP331278). The protein was produced from HEK293T cells transfected with CCDC169-SOHLH2 cDNA clone (Cat# RC231278) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC169-SOHLH2
• Synonyms: C13orf38-SOHLH2; SOHLH2; TEB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231278 representing NM_001198910
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTCTTCAGGAATCCTTAAACACATTACTTAAACAGCTAGAAGAAGAAAAGAAGACTCTTGAAA
  GTCAAGTGAAATACTATGCACTTAAACTGGAACAAGAATCAAAGGCTTACCAGAAGATCAACAATGAACG
  CCGTACATACCTAGCTGAAATGTCTCAGGGTTCTGGTTTACATCAAGTTTCTAAAAGGCAACAGGTGGAT
  CAACTGCCTAGGATGCAAGAGAATCTAGTGAAAACCCTCCTCTTAAAAGAAGAATTGGACCCTTTAAAGG
  CAAAAATAGACATCTTATTAGTTGGAGATGTCACTGTGGGCTACCTGGCTGATACTGTACAGAAACTATT
  TGCAAACATAGCAGAAGTCACCATCACCATCAGTGACACGAAGGAGGCAGCAGCGCTTTTGGATGATTGC
  ATATTCAACATGGTTCTCTTGAAGGTGCCTTCTTCACTAAGTGCCGAGGAGCTGGAAGCCATCAAGTTAA
  TTAGATTTGGCAAAAAGAAAAATACACATTCACTGTTTGTTTTTATAATCCCTGAAAATTTTAAAGGTTG
  TATTTCAGGGCATGGAATGGATATTGCTTTAACTGAACCACTGACAATGGAAAAAATGAGTAATGTGGTA
  AAATACTGGACAACATGTCCCTCAAACACTGTTAAGACTGAAAACGCAACTGGGCCTGAAGAACTTGGAT
  TGCCCCTGCAGAGGTCCTACAGCGAACACCTGGGATATTTTCCTACTGATCTATTTGCCTGCTCTGAATC
  TTTAAGGAATGGCAATGGGCTTGAATTAAATGCTTCGTTGTCAGAGTTCGAGAAAAACAAAAAGATCTCT
  CTTCTTCATTCAAGCAAGGAAAAACTAAGAAGGGAAAGAATCAAATATTGCTGTGAGCAGCTGCGTACTC
  TCTTGCCGTATGTAAAAGGGAGAAAGAATGATGCGGCTTCAGTTCTTGAGGCAACAGTTGATTATGTGAA
  ATATATCCGGGAGAAAATCTCTCCAGCCGTTATGGCCCAGATTACAGAAGCACTTCAGAGCAACATGAGG
  TTTTGTAAGAAACAACAAACACCCATTGAGCTGTCTCTCCCAGGCACTGTCATGGCACAGCGGGAAAACA
  GTGTGATGAGCACTTACTCCCCTGAGAGAGGGCTCCAATTCCTGACTAATACGTGCTGGAATGGGTGCTC
  CACTCCTGATGCAGAGAGCTCCTTGGATGAAGCTGTGAGAGTTCCATCAAGCTCCGCCTCAGAGAATGCT
  ATTGGTGATCCATATAAAACTCACATTTCCAGTGCAGCGCTGTCTCTGAATTCCTTGCATACTGTCAGAT
  ATTATTCTAAAGTCACCCCTTCCTACGATGCAACTGCTGTAACAAATCAGAACATTTCAATTCATTTACC
  TTCAGCCATGCCCCCGGTCTCAAAGCTTCTCCCTCGGCACTGCACTTCTGGGTTGGGCCAGACGTGCACT
  ACACATCCCAACTGTCTGCAACAGTTTTGGGCGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231278 representing NM_001198910
  Red=Cloning site Green=Tags(s)
  
  METLQESLNTLLKQLEEEKKTLESQVKYYALKLEQESKAYQKINNERRTYLAEMSQGSGLHQVSKRQQVD
  QLPRMQENLVKTLLLKEELDPLKAKIDILLVGDVTVGYLADTVQKLFANIAEVTITISDTKEAAALLDDC
  IFNMVLLKVPSSLSAEELEAIKLIRFGKKKNTHSLFVFIIPENFKGCISGHGMDIALTEPLTMEKMSNVV
  KYWTTCPSNTVKTENATGPEELGLPLQRSYSEHLGYFPTDLFACSESLRNGNGLELNASLSEFEKNKKIS
  LLHSSKEKLRRERIKYCCEQLRTLLPYVKGRKNDAASVLEATVDYVKYIREKISPAVMAQITEALQSNMR
  FCKKQQTPIELSLPGTVMAQRENSVMSTYSPERGLQFLTNTCWNGCSTPDAESSLDEAVRVPSSSASENA
  IGDPYKTHISSAALSLNSLHTVRYYSKVTPSYDATAVTNQNISIHLPSAMPPVSKLLPRHCTSGLGQTCT
  THPNCLQQFWAY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198910
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198910.1, NP_001185839.1
• RefSeq ORF: 1509
• Locus ID: 100526761
• MW: 56.6 kDa
• Gene Summary: This locus represents naturally occurring read-through transcription between the neighboring C13orf38 (chromosome 13 open reading frame 38) and SOHLH2 (spermatogenesis and oogenesis specific basic helix-loop-helix 2) genes. The read-through transcript encodes a fusion protein that shares sequence identity with the products of each individual gene. [provided by RefSeq, Nov 2010]