SLC6A13 (NM_001190997) Human Tagged ORF Clone

CAT#: RC231283

SLC6A13 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, GABA), member 13 (SLC6A13), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY434282) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231283 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC6A13
• Synonyms: GAT-2; GAT2; GAT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231283 representing NM_001190997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGCAGGGTCTCAGGCACAACCAGTAATGGAGAGACAAAACCAGTGTATCCAGTCATGGAAAAGA
  AGGAGGAAGATGGCACCCTGGAGCGGGGGCACTGGAACAACAAGATGGAGTTTGTGCTGTCAGTGGCTGG
  GGAGATCATTGGCTTAGGCAACGTCTGGAGGTTTCCCTATCTCTGCTACAAAAATGGGGGAGAACACTGT
  ATGGAGTTCCAGAAGACCAACGGCTCCCTGAATGGTACCTCTGAGAATGCCACCTCTCCTGTCATCGAGT
  TCTGGGAGCGGCGGGTCTTGAAGATCTCTGATGGGATCCAGCACCTGGGGGCCCTGCGCTGGGAGCTGGC
  TCTGTGCCTCCTGCTGGCCTGGGTCATCTGCTACTTCTGCATCTGGAAGGGGGTGAAGTCCACAGGCAAG
  GTGGTGTACTTCACGGCCACATTTCCTTACCTCATGCTGGTGGTCCTGTTAATTCGAGGGGTGACGTTGC
  CTGGGGCAGCCCAAGGAATTCAGTTTTACCTGTACCCAAACCTCACGCGTCTGTGGGATCCCCAGGTGTG
  GATGGATGCAGGCACCCAGATATTCTTCTCCTTCGCCATCTGTCTTGGGTGCCTGACAGCCCTGGGCAGC
  TACAACAAGTACCACAACAACTGCTACAGGGACTGCATCGCCCTCTGCTTCCTCAACAGCGGCACCAGCT
  TTGTGGCCGGCTTTGCCATCTTCTCCATCCTGGGCTTCATGTCTCAGGAGCAGGGGGTGCCCATTTCTGA
  GGTGGCCGAGTCAGGCCCTGGCCTGGCTTTCATCGCTTACCCGCGGGCTGTGGTGATGCTGCCCTTCTCT
  CCTCTCTGGGCCTGCTGTTTCTTCTTCATGGTCGTTCTCCTGGGACTGGATAGCCAGTTTGTGTGTGTAG
  AAAGCCTGGTGACAGCGCTGGTGGACATGTACCCTCACGTGTTCCGCAAGAAGAACCGGAGGGAAGTCCT
  CATCCTTGGAGTATCTGTCGTCTCCTTCCTTGTGGGGCTGATCATGCTCACAGAGGGCGGAATGTACGTG
  TTCCAGCTCTTTGACTACTATGCGGCCAGTGGCATGTGCCTCCTGTTCGTGGCCATCTTCGAGTCCCTCT
  GTGTGGCTTGGGTTTACGGAGCCAAGCGCTTCTACGACAACATCGAAGACATGATTGGGTACAGGCCATG
  GCCTCTTATCAAATACTGTTGGCTCTTCCTCACACCAGCTGTGTGCACAGCCACCTTTCTCTTCTCCCTG
  ATAAAGTACACTCCGCTGACCTACAACAAGAAGTACACGTACCCGTGGTGGGGCGATGCCCTGGGCTGGC
  TCCTGGCTCTGTCCTCCATGGTCTGCATTCCTGCCTGGAGCCTCTACAGACTCGGAACCCTCAAGGGCCC
  CTTCAGAGAGAGAATCCGTCAGCTCATGTGCCCAGCCGAGGACCTGCCCCAGCGGAACCCAGCAGGACCC
  TCGGCTCCCGCCACCCCCAGGACCTCACTGCTCAGACTCACAGAGCTAGAGTCTCACTGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231283 representing NM_001190997
  Red=Cloning site Green=Tags(s)
  
  MDSRVSGTTSNGETKPVYPVMEKKEEDGTLERGHWNNKMEFVLSVAGEIIGLGNVWRFPYLCYKNGGEHC
  MEFQKTNGSLNGTSENATSPVIEFWERRVLKISDGIQHLGALRWELALCLLLAWVICYFCIWKGVKSTGK
  VVYFTATFPYLMLVVLLIRGVTLPGAAQGIQFYLYPNLTRLWDPQVWMDAGTQIFFSFAICLGCLTALGS
  YNKYHNNCYRDCIALCFLNSGTSFVAGFAIFSILGFMSQEQGVPISEVAESGPGLAFIAYPRAVVMLPFS
  PLWACCFFFMVVLLGLDSQFVCVESLVTALVDMYPHVFRKKNRREVLILGVSVVSFLVGLIMLTEGGMYV
  FQLFDYYAASGMCLLFVAIFESLCVAWVYGAKRFYDNIEDMIGYRPWPLIKYCWLFLTPAVCTATFLFSL
  IKYTPLTYNKKYTYPWWGDALGWLLALSSMVCIPAWSLYRLGTLKGPFRERIRQLMCPAEDLPQRNPAGP
  SAPATPRTSLLRLTELESHC
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190997
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190997.1, NM_001190997.2, NP_001177926.1
• RefSeq ORF: 1533 bp
• Locus ID: 6540
• Cytogenetics: 12p13.33
• Protein Families: Druggable Genome, Transmembrane
• MW: 58 kDa
• Gene Summary: ''