PODN (NM_001199082) Human Tagged ORF Clone

CAT#: RC231292

PODN (Myc-DDK-tagged)-Human podocan (PODN), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY434291) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231292 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PODN
• Synonyms: PCAN; SLRR5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231292 representing NM_001199082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGAGCCCGAGCCCGCGGAGCGCAGCTGAGACTGGGGGAGCGCGTTCGGCCTGTGGGGCGCCGCT
  CGGCGCCGGGGCGCAGCAGGTTCCGTCAGCCCTGGCGCCCAGGCGCATCTGACTCGGCACCCCCTGCAGG
  CACCATGGCCCAGAGCCGGGTGCTGCTGCTCCTGCTGCTGCTGCCGCCACAGCTGCACCTGGGACCTGTG
  CTTGCCGTGAGGGCCCCAGGATTTGGCCGAAGTGGCGGCCACAGCCTGAGCCCCGAAGAGAACGAATTTG
  CGGAGGAGGAGCCGGTGCTGGTACTGAGCCCTGAGGAGCCCGGGCCTGGCCCAGCCGCGGTCAGCTGCCC
  CCGAGACTGTGCCTGTTCCCAGGAGGGCGTCGTGGACTGTGGCGGTATTGACCTGCGTGAGTTCCCGGGG
  GACCTGCCTGAGCACACCAACCACCTATCTCTGCAGAACAACAAGCTGGAGAAGATCCCCCCGGGGGCCT
  TCAGCGAGCTGAGCAGCCTGCGCGAGCTATACCTGCAGAACAACTACCTGACTGACGAGGGCCTGGACAA
  CGAGACCTTCTGGAAGCTCTCCAGCCTGGAGTACCTGGATCTGTCCAGCAACAACCTGTCTCGGGTCCCA
  GCTGGGCTGCCGCGCAGCCTGGTGCTGCTGCACTTGGAGAAGAACGCCATCCGGAGCGTGGACGCGAATG
  TGCTGACCCCCATCCGCAGCCTGGAGTACCTGCTGCTGCACAGCAACCAGCTGCGGGAGCAGGGCATCCA
  CCCACTGGCCTTCCAGGGCCTCAAGCGGTTGCACACGGTGCACCTGTACAACAACGCGCTGGAGCGCGTG
  CCCAGTGGCCTGCCTCGCCGCGTGCGCACCCTCATGATCCTGCACAACCAGATCACAGGCATTGGCCGCG
  AAGACTTTGCCACCACCTACTTCCTGGAGGAGCTCAACCTCAGCTACAACCGCATCACCAGCCCGCAGGT
  GCACCGCGACGCCTTCCGCAAGCTGCGCCTGCTGCGCTCGCTGGACCTGTCGGGCAACCGGCTGCACACG
  CTGCCACCTGGGCTGCCTCGAAATGTCCATGTGCTGAAGGTCAAGCGCAATGAGCTGGCTGCCTTGGCAC
  GAGGGGCGCTGGTGGGCATGGCTCAGCTGCGTGAGCTGTACCTCACCAGCAACCGACTGCGCAGCCGAGC
  CCTGGGCCCCCGTGCCTGGGTGGACCTCGCCCATCTGCAGCTGCTGGACATCGCCGGGAATCAGCTCACA
  GAGATCCCCGAGGGGCTCCCCGAGTCACTTGAGTACCTGTACCTGCAGAACAACAAGATTAGTGCGGTGC
  CCGCCAATGCCTTCGACTCCACGCCCAACCTCAAGGGGATCTTTCTCAGGTTTAACAAGCTGGCTGTGGG
  CTCCGTGGTGGACAGTGCCTTCCGGAGGCTGAAGCACCTGCAGGTCTTGGACATTGAAGGCAACTTAGAG
  TTTGGTGACATTTCCAAGGACCGTGGCCGCTTGGGGAAGGAAAAGGAGGAGGAGGAAGAGGAGGAGGAGG
  AGGAAGAGGAAACAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231292 representing NM_001199082
  Red=Cloning site Green=Tags(s)
  
  MEGARARGAQLRLGERVRPVGRRSAPGRSRFRQPWRPGASDSAPPAGTMAQSRVLLLLLLLPPQLHLGPV
  LAVRAPGFGRSGGHSLSPEENEFAEEEPVLVLSPEEPGPGPAAVSCPRDCACSQEGVVDCGGIDLREFPG
  DLPEHTNHLSLQNNKLEKIPPGAFSELSSLRELYLQNNYLTDEGLDNETFWKLSSLEYLDLSSNNLSRVP
  AGLPRSLVLLHLEKNAIRSVDANVLTPIRSLEYLLLHSNQLREQGIHPLAFQGLKRLHTVHLYNNALERV
  PSGLPRRVRTLMILHNQITGIGREDFATTYFLEELNLSYNRITSPQVHRDAFRKLRLLRSLDLSGNRLHT
  LPPGLPRNVHVLKVKRNELAALARGALVGMAQLRELYLTSNRLRSRALGPRAWVDLAHLQLLDIAGNQLT
  EIPEGLPESLEYLYLQNNKISAVPANAFDSTPNLKGIFLRFNKLAVGSVVDSAFRRLKHLQVLDIEGNLE
  FGDISKDRGRLGKEKEEEEEEEEEEEETR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199082
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001199082.1, NP_001186011.1
• RefSeq ORF: 1560
• Locus ID: 127435
• Protein Families: Secreted Protein
• MW: 58.3 kDa
• Gene Summary: The protein encoded by this gene is a member of the small leucine-rich repeat protein family and contains an amino terminal CX3CXCX7C cysteine-rich cluster followed by a leucine-rich repeat domain. Studies suggest that this protein could function to inhibit smooth muscle cell proliferation and migration following arterial injury. [provided by RefSeq, Jul 2016]