ATG16L1 (NM_001190266) Human Tagged ORF Clone

CAT#: RC231296

ATG16L1 (Myc-DDK-tagged)-Human ATG16 autophagy related 16-like 1 (S. cerevisiae) (ATG16L1), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY434295) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231296 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATG16L1
• Synonyms: APG16L; ATG16A; ATG16L; IBD10; WDR30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231296 representing NM_001190266
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAACTGAGGATTAAGCACCAAGAGGAACTGACTGAATTACACAAGAAACGTGGGGAGTTAGCTC
  AACTGGTGATTGACCTGAATAACCAAATGCAGCGGAAGGACAGGGAGATGCAGATGAATGAAGCAAAAAT
  TGCAGAATGTTTGCAGACTATCTCTGACCTGGAGACGGAGTGCCTAGACCTGCGCACTAAGCTTTGTGAC
  CTTGAAAGAGCCAACCAGACCCTGAAGGATGAATATGATGCCCTGCAGATCACTTTTACTGCCTTGGAGG
  GAAAACTGAGGAAAACTACGGAAGAGAACCAGGAGCTGGTCACCAGATGGATGGCTGAGAAAGCCCAGGA
  AGCCAATCGGCTTAATGCAGAGAATGAAAAAGACTCCAGGAGGCGGCAAGCCCGGCTGCAGAAAGAGCTT
  GCAGAAGCAGCAAAGGAACCTCTACCAGTCGAACAGGATGATGACATTGAGGTCATTGTGGATGAAACTT
  CTGATCACACAGAAGAGACCTCTCCTGTGCGAGCCATCAGCAGAGCAGCCACTAAGCGACTCTCGCAGCC
  TGCTGGAGGCCTTCTGGATTCTATCACTAATATCTTTGGGAGACGCTCTGTCTCTTCCTTCCCAGTCCCC
  CAGGACAATGTGGATACTCATCCTGGTTCTGGTAAAGAAGTGAGGGTACCAGCTACTGCCTTGTGTGTCT
  TCGATGCACATGATGGGGAAGTCAACGCTGTGCAGTTCAGTCCAGGTTCCCGGTTACTGGCCACTGGAGG
  CATGGACCGCAGGGTTAAGCTTTGGGAAGTATTTGGAGAAAAATGTGAGTTCAAGGGTTCCCTATCTGGC
  AGTAATGCAGGAATTACAAGCATTGAATTTGATAGTGCTGGATCTTACCTCTTAGCAGCTTCAAATGATT
  TTGCAAGCCGAATCTGGACTGTGGATGATTATCGATTACGGCACACACTCACGGGACACAGTGGGAAAGT
  GCTGTCTGCTAAGTTCCTGCTGGACAATGCGCGGATTGTCTCAGGAAGTCACGACCGGACTCTCAAACTC
  TGGGATCTACGCAGCAAAGTCTGCATAAAGACAGTGTTTGCAGGATCCAGTTGCAATGATATTGTCTGCA
  CAGAGCAATGTGTAATGAGTGGACATTTTGACAAGAAAATTCGTTTCTGGGACATTCGATCAGAGAGCAT
  AGTTCGAGAGATGGAGCTGTTGGGAAAGATTACTGCCCTGGACTTAAACCCAGAAAGGACTGAGCTCCTG
  AGCTGCTCCCGTGATGACTTGCTAAAAGTTATTGATCTCCGAACAAATGCTATCAAGCAGACATTCAGTG
  CACCTGGGTTCAAGTGCGGCTCTGACTGGACCAGAGTTGTCTTCAGCCCTGATGGCAGTTACGTGGCGGC
  AGGCTCTGCTGAGGGCTCTCTGTATATCTGGAGTGTGCTCACAGGGAAAGTGGAAAAGGTTCTTTCAAAG
  CAGCACAGCTCATCCATCAATGCGGTGGCGTGGTCGCCCTCTGGCTCGCACGTTGTCAGTGTGGACAAAG
  GATGCAAAGCTGTGCTGTGGGCACAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231296 representing NM_001190266
  Red=Cloning site Green=Tags(s)
  
  MAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQRKDREMQMNEAKIAECLQTISDLETECLDLRTKLCD
  LERANQTLKDEYDALQITFTALEGKLRKTTEENQELVTRWMAEKAQEANRLNAENEKDSRRRQARLQKEL
  AEAAKEPLPVEQDDDIEVIVDETSDHTEETSPVRAISRAATKRLSQPAGGLLDSITNIFGRRSVSSFPVP
  QDNVDTHPGSGKEVRVPATALCVFDAHDGEVNAVQFSPGSRLLATGGMDRRVKLWEVFGEKCEFKGSLSG
  SNAGITSIEFDSAGSYLLAASNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKL
  WDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESIVREMELLGKITALDLNPERTELL
  SCSRDDLLKVIDLRTNAIKQTFSAPGFKCGSDWTRVVFSPDGSYVAAGSAEGSLYIWSVLTGKVEKVLSK
  QHSSSINAVAWSPSGSHVVSVDKGCKAVLWAQY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190266
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001190266.1, NP_001177195.1
• RefSeq ORF: 1572
• Locus ID: 55054
• MW: 58.7 kDa
• Gene Summary: The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]