NOL4 (NM_001198547) Human Tagged ORF Clone

CAT#: RC231315

NOL4 (Myc-DDK-tagged)-Human nucleolar protein 4 (NOL4), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY434314) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231315 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NOL4
• Synonyms: CT125; HRIHFB2255; NOLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231315 representing NM_001198547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATCTGATGCAGGAGACCTTTCTGCATCATGCCACGGATGGCGTAGGGGTAGATGAGAAGCTAT
  CTTTACGACGGGTAGCTGTGGTTGAAGATTTCTTTGACATTATTTATTCGATGCATGTGGAAACGGGGCC
  AAATGGAGAACAAATTCGGAAACACGCTGGACAAAAGAGAACTTACAAAGCAATTTCAGAGAGCTATGCC
  TTCCTACCAAGAGAAGCGGTGACACGATTTCTAATGAGCTGCTCAGAGTGCCAGAAAAGAATGCATTTAA
  ACCCAGATGGAACAGATCATAAAGATAATGGAAAACCTCCCACTTTGGTGACCAGCATGATTGACTACAA
  CATGCCAATTACCATGGCCTACATGAAACACATGAAGCTGCAGCTGCTAAACTCACAGCAAGATGAGGAT
  GAAAGTTCAATAGAAAGTGATGAATTTGACATGAGTGATTCAACACGGATGTCAGCTGTGAACTCTGATC
  TTAGCTCCAATCTTGAAGAAAGAATGCAAAGTCCCCAGAATCTTCATGGCCAGCAAGATGATGATTCTGC
  TGCAGAGAGCTTTAATGGCAATGAGACTCTGGGGCACAGTTCAATTGCTTCAGGGGGAACACACAGCAGG
  GAGATGGGAGACTCCAACAGTGATGGCAAAACTGGGCTGGAGCAAGATGAACAGCCACTGAACCTGAGTG
  ACAGTCCCCTCTCTGCGCAGCTAACTTCGGAATACAGAATAGATGATCACAACAGTAATGGGAAAAACAA
  GTATAAGAATCTTCTAATTTCTGACCTCAAGATGGAACGAGAGGCGAGAGAAAATGGAAGCAAGTCTCCT
  GCACATAGTTACTCCAGCTATGACTCTGGCAAAAATGAGAGTGTAGACCGAGGAGCTGAGGACCTCTCAC
  TAAACAGGGGAGATGAGGACGAAGATGACCACGAGGACCATGACGATTCGGAGAAAGTTAATGAGACAGA
  CGGCGTTGAAGCCGAGCGGCTGAAAGCTTTTAATATGTTTGTCAGGCTGTTTGTAGATGAAAACTTGGAC
  CGAATGGTCCCAATCTCTAAGCAGCCCAAAGAAAAGATCCAGGCTATCATTGACTCATGCAGGCGACAAT
  TCCCTGAGTATCAAGAGCGTGCCAGAAAACGTATACGTACTTACCTCAAGTCCTGCAGGCGGATGAAAAG
  AAGTGGTTTTGAGATGTCTCGACCTATTCCTTCCCACCTTACTTCAGCAGTTGCAGAGAGTATCTTGGCT
  TCAGCTTGTGAGAGTGAGAGTAGAAATGCCGCCAAGAGGATGCGTCTGGAGAGACAGCAGGATGAGTCTG
  CTCCAGCTGACAAACAGTGTAAACCAGAGGCGACCCAGGCCACTTACTCAACATCAGCTGTTCCAGGCTC
  ACAGGACGTGCTGTACATCAATGGAAATGGGACCTATAGTTACCATAGTTACAGAGGGCTAGGAGGGGGT
  CTGCTAAATCTGAATGATGCTTCCAGCAGTGGACCCACTGATCTCAGCATGAAGAGACAATTGGCGACTA
  GCTCAGGATCCTCCAGCAGCTCAAACTCCAGACCCCAGCTGAGTCCAACTGAAATCAATGCCGTGAGACA
  GCTTGTTGCAGGATATCGAGAATCAGCTGCATTTTTATTGCGATCTGCAGATGAACTGGAAAATCTCATT
  TTACAACAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231315 representing NM_001198547
  Red=Cloning site Green=Tags(s)
  
  MADLMQETFLHHATDGVGVDEKLSLRRVAVVEDFFDIIYSMHVETGPNGEQIRKHAGQKRTYKAISESYA
  FLPREAVTRFLMSCSECQKRMHLNPDGTDHKDNGKPPTLVTSMIDYNMPITMAYMKHMKLQLLNSQQDED
  ESSIESDEFDMSDSTRMSAVNSDLSSNLEERMQSPQNLHGQQDDDSAAESFNGNETLGHSSIASGGTHSR
  EMGDSNSDGKTGLEQDEQPLNLSDSPLSAQLTSEYRIDDHNSNGKNKYKNLLISDLKMEREARENGSKSP
  AHSYSSYDSGKNESVDRGAEDLSLNRGDEDEDDHEDHDDSEKVNETDGVEAERLKAFNMFVRLFVDENLD
  RMVPISKQPKEKIQAIIDSCRRQFPEYQERARKRIRTYLKSCRRMKRSGFEMSRPIPSHLTSAVAESILA
  SACESESRNAAKRMRLERQQDESAPADKQCKPEATQATYSTSAVPGSQDVLYINGNGTYSYHSYRGLGGG
  LLNLNDASSSGPTDLSMKRQLATSSGSSSSSNSRPQLSPTEINAVRQLVAGYRESAAFLLRSADELENLI
  LQQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198547
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198547.1, NP_001185476.1
• RefSeq ORF: 1695
• Locus ID: 8715
• MW: 63.4 kDa