DDX3 (DDX3X) (NM_001193417) Human Tagged ORF Clone

CAT#: RC231353

DDX3X (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked (DDX3X), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDX3X
• Synonyms: CAP-Rf; DBX; DDX3; DDX14; HLP2; MRX102
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231353 representing NM_001193417
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCATGTGGCAGTGGAAAATGCGCTCGGGCTGGACCAGCAGTTTGCTGGCCTAGACCTGAACTCTT
  CAGATAATCAGAGTGGAGGAAGTACAGCCAGCAGTTTCTACGATAAAGACAGTTCAGGGTGGAGTTCTAG
  CAAAGATAAGGATGCGTATAGCAGTTTTGGATCTCGTAGTGATTCAAGAGGGAAGTCTAGCTTCTTCAGT
  GATCGTGGAAGTGGATCAAGGGGAAGGTTTGATGATCGTGGACGGAGTGATTACGATGGCATTGGCAGCC
  GTGGTGACAGAAGTGGCTTTGGCAAATTTGAACGTGGTGGAAACAGTCGCTGGTGTGACAAATCAGATGA
  AGATGATTGGTCAAAACCACTCCCACCAAGTGAACGCTTGGAACAGGAACTCTTTTCTGGAGGCAACACT
  GGGATTAATTTTGAGAAATACGATGACATTCCAGTTGAGGCAACAGGCAACAACTGTCCTCCACATATTG
  AAAGTTTCAGTGATGTTGAGATGGGAGAAATTATCATGGGAAACATTGAGCTTACTCGTTATACTCGCCC
  AACTCCAGTGCAAAAGCATGCTATTCCTATTATCAAAGAGAAAAGAGACTTGATGGCTTGTGCCCAAACA
  GGGTCTGGAAAAACTGCAGCATTTCTGTTGCCCATCTTGAGTCAGATTTATTCAGATGGTCCAGGCGAGG
  CTTTGAGGGCCATGAAGGAAAATGGAAGGTATGGGCGCCGCAAACAATACCCAATCTCCTTGGTATTAGC
  ACCAACGAGAGAGTTGGCAGTACAGATCTACGAGGAAGCCAGAAAATTTTCATACCGATCTAGAGTTCGT
  CCTTGCGTGGTTTATGGTGGTGCCGATATTGGTCAGCAGATTCGAGACTTGGAACGTGGATGCCATTTGT
  TAGTAGCCACTCCAGGACGTCTAGTGGATATGATGGAAAGAGGAAAGATTGGATTAGACTTTTGCAAATA
  CTTGGTGTTAGATGAAGCTGATCGGATGTTGGATATGGGGTTTGAGCCTCAGATTCGTAGAATAGTCGAA
  CAAGATACTATGCCTCCAAAGGGTGTCCGCCACACTATGATGTTTAGTGCTACTTTTCCTAAGGAAATAC
  AGATGCTGGCTCGTGATTTCTTAGATGAATATATCTTCTTGGCTGTAGGAAGAGTTGGCTCTACCTCTGA
  AAACATCACACAGAAAGTAGTTTGGGTGGAAGAATCAGACAAACGGTCATTTCTGCTTGACCTCCTAAAT
  GCAACAGGCAAGGATTCACTGACCTTAGTGTTTGTGGAGACCAAAAAGGGTGCAGATTCTCTGGAGGATT
  TCTTATACCATGAAGGATACGCATGTACCAGCATCCATGGAGACCGTTCTCAGAGGGATAGAGAAGAGGC
  CCTTCACCAGTTCCGCTCAGGAAAAAGCCCAATTTTAGTGGCTACAGCAGTAGCAGCAAGAGGACTGGAC
  ATTTCAAATGTGAAACATGTTATCAATTTTGACTTGCCAAGTGATATTGAAGAATATGTACATCGTATTG
  GTCGTACGGGACGTGTAGGAAACCTTGGCCTGGCAACCTCATTCTTTAACGAGAGGAACATAAATATTAC
  TAAGGATTTGTTGGATCTTCTTGTTGAAGCTAAACAAGAAGTGCCGTCTTGGTTAGAAAACATGGCTTAT
  GAACACCACTACAAGGGTAGCAGTCGTGGACGTTCTAAGAGTAGCAGATTTAGTGGAGGGTTTGGTGCCA
  GAGACTACCGACAAAGTAGCGGTGCCAGCAGTTCCAGCTTCAGCAGCAGCCGCGCAAGCAGCAGCCGCAG
  TGGCGGAGGTGGCCACGGTAGCAGCAGAGGATTTGGTGGAGGTGGCTATGGAGGCTTTTACAACAGTGAT
  GGATATGGAGGAAATTATAACTCCCAGGGGGTTGACTGGTGGGGTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231353 representing NM_001193417
  Red=Cloning site Green=Tags(s)
  
  MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASSFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFS
  DRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNT
  GINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQT
  GSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVR
  PCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVE
  QDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLN
  ATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLD
  ISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAY
  EHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSD
  GYGGNYNSQGVDWWGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193417
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193417.1, NM_001193417.2, NP_001180346.1
• RefSeq ORF: 1941 bp
• Locus ID: 1654
• Cytogenetics: Xp11.4
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 71.8 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]'