TAF6 (NM_001190415) Human Tagged ORF Clone

CAT#: RC231390

TAF6 (Myc-DDK-tagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF6
• Synonyms: ALYUS; MGC:8964; TAF(II)70; TAF(II)80; TAF2E; TAFII-70; TAFII-80; TAFII70; TAFII80; TAFII85
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231390 representing NM_001190415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAAGCGCCGTCTACAAACAATCACCGCTTGCCTTCAACTTCGGGAAGGGTCTCCGTCTCTCCACC
  GGGGGCTTCATCCTTCCAGGGAGGAGAAGAGGGACTCCAGAATGGCTGAGGAGAAGAAGCTGAAGCTTAG
  CAACACTGTGCTGCCCTCGGAGTCCATGAAGGTGGTGGCTGAATCCATGGGCATCGCCCAGATTCAGGAG
  GAGACCTGCCAGCTGCTAACGGATGAGGTCAGCTACCGCATCAAAGAGATCGCACAGGATGCCTTGAAGT
  TCATGCACATGGGGAAGCGGCAGAAGCTCACCACCAGTGACATTGACTACGCCTTGAAGCTAAAGAATGT
  CGAGCCACTCTATGGCTTCCACGCCCAGGAGTTCATTCCTTTCCGCTTCGCCTCTGGTGGGGGCCGGGAG
  CTTTACTTCTATGAGGAGAAGGAGGTTGATCTGAGCGACATCATCAATACCCCTCTGCCCCGGGTGCCCC
  TGGACGTCTGCCTCAAAGCTCATTGGCTGAGCATCGAGGGCTGCCAGCCAGCTATCCCCGAGAACCCGCC
  CCCAGCTCCCAAAGAGCAACAGAAGGCTGAAGCCACAGAACCCCTGAAGTCAGCCAAGCCAGGCCAGGAG
  GAAGACGGACCCCTGAAGGGCAAAGGTCAAGGGGCCACCACAGCCGACGGCAAAGGGAAAGAGAAGAAGG
  CGCCGCCCTTGCTGGAGGGGGCCCCCTTGCGACTGAAGCCCCGGAGCATCCACGAGTTGTCTGTGGAGCA
  GCAGCTCTACTACAAGGAGATCACCGAGGCCTGCGTGGGCTCCTGCGAGGCCAAGAGGGCGGAAGCCCTG
  CAAAGCATTGCCACGGACCCTGGACTGTATCAGATGCTGCCACGGTTCAGTACCTTTATCTCGGAGGGGG
  TCCGTGTGAACGTGGTTCAGAACAACCTGGCCCTACTCATCTACCTGATGCGTATGGTGAAAGCGCTGAT
  GGACAACCCCACGCTCTATCTAGAAAAATACGTCCATGAGCTGATTCCAGCTGTGATGACCTGCATCGTG
  AGCAGACAGTTGTGCCTGCGACCAGATGTGGACAATCACTGGGCACTCCGAGACTTTGCTGCCCGCCTGG
  TGGCCCAGATCTGCAAGCATTTTAGCACAACCACTAACAACATCCAGTCCCGGATCACCAAGACCTTCAC
  CAAGAGCTGGGTGGACGAGAAGACGCCCTGGACGACTCGTTATGGCTCCATCGCAGGCTTGGCTGAGCTG
  GGACACGATGTTATCAAGACTCTGATTCTGCCCCGGCTGCAGCAGGAAGGGGAGCGGATCCGCAGTGTGC
  TGGACGGCCCTGTGCTGAGCAACATTGACCGGATTGGAGCAGACCATGTGCAGAGCCTCCTGCTGAAACA
  CTGTGCTCCTGTTCTGGCAAAGCTGCGCCCACCGCCTGACAATCAGGACGCCTATCGGGCAGAATTCGGG
  TCCCTTGGGCCCCTCCTCTGCTCCCAGGTGGTCAAGGCTCGGGCCCAGGCTGCTCTGCAGGCTCAGCAGG
  TCAACAGGACCACTCTGACCATCACGCAGCCCCGGCCCACGCTGACCCTCTCGCAGGCCCCACAGCCTGG
  CCCTCGCACCCCTGGCTTGCTGAAGGTTCCTGGCTCCATCGCACTTCCTGTCCAGACACTGGTGTCTGCA
  CGAGCGGCTGCCCCACCACAGCCTTCCCCTCCTCCAACCAAGTTTATTGTAATGTCATCGTCCTCCAGCG
  CCCCATCCACCCAGCAGGTCCTGTCCCTCAGCACCTCGGCCCCCGGCTCAGGTTCCACCACCACTTCGCC
  CGTCACCACCACCGTCCCCAGCGTGCAGCCCATCGTCAAGTTGGTCTCCACCGCCACCACCGCACCCCCC
  AGCACTGCTCCCTCTGGTCCTGGGAGTGTCCAGAAGTACATCGTGGTCTCACTTCCCCCAACAGGGGAGG
  GCAAAGGAGGCCCCACCTCCCATCCTTCTCCAGTTCCTCCCCCGGCATCGTCCCCGTCCCCACTCAGCGG
  CAGTGCCCTTTGTGGGGGGAAGCAGGAGGCTGGGGACAGTCCCCCTCCAGCTCCAGGGACTCCAAAAGCC
  AATGGCTCCCAGCCCAACTCCGGCTCCCCTCAGCCTGCTCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231390 representing NM_001190415
  Red=Cloning site Green=Tags(s)
  
  MTKRRLQTITACLQLREGSPSLHRGLHPSREEKRDSRMAEEKKLKLSNTVLPSESMKVVAESMGIAQIQE
  ETCQLLTDEVSYRIKEIAQDALKFMHMGKRQKLTTSDIDYALKLKNVEPLYGFHAQEFIPFRFASGGGRE
  LYFYEEKEVDLSDIINTPLPRVPLDVCLKAHWLSIEGCQPAIPENPPPAPKEQQKAEATEPLKSAKPGQE
  EDGPLKGKGQGATTADGKGKEKKAPPLLEGAPLRLKPRSIHELSVEQQLYYKEITEACVGSCEAKRAEAL
  QSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLIYLMRMVKALMDNPTLYLEKYVHELIPAVMTCIV
  SRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNNIQSRITKTFTKSWVDEKTPWTTRYGSIAGLAEL
  GHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGADHVQSLLLKHCAPVLAKLRPPPDNQDAYRAEFG
  SLGPLLCSQVVKARAQAALQAQQVNRTTLTITQPRPTLTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSA
  RAAAPPQPSPPPTKFIVMSSSSSAPSTQQVLSLSTSAPGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPP
  STAPSGPGSVQKYIVVSLPPTGEGKGGPTSHPSPVPPPASSPSPLSGSALCGGKQEAGDSPPPAPGTPKA
  NGSQPNSGSPQPAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190415
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190415.1, NP_001177344.1
• RefSeq ORF: 2145 bp
• Locus ID: 6878
• Cytogenetics: 7q22.1
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 77.4 kDa
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]'