PDE1C (NM_001191058) Human Tagged ORF Clone

CAT#: RC231408

PDE1C (Myc-DDK-tagged)-Human phosphodiesterase 1C, calmodulin-dependent 70kDa (PDE1C), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE1C
• Synonyms: cam-PDE 1C; DFNA74; hCam-3; Hcam3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231408 representing NM_001191058
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGACGCCGGCAACAGGAAGGAGGGCTTCAAAAAATGCCGGAGCGCCACTTTCAGCATCGATGGCT
  ACAGCTTCACCATAGTTGCAAATGAAGCTGGAGACAAGAATGCCAGACCACTGGCCCGCTTCTCTCGGTC
  TAAGTCACAGAACTGTCTGTGGAACTCCCTCATCGATGGGCTCACAGGGAATGTCAAGGAGAAGCCAAGG
  CCAACAATTGTCCATGACCCTCGACCCCCAGAGGAGATCCTAGCTGATGAATTGCCACAGCTGGATAGCT
  CAGAAGTCTTGGTGAAGACATCCTTCAGATTACGGTCTTTGGTCAAACAATTAGAGAGAGGGGAAGCTTC
  AGTGGTAGATCTTAAGAAGAATTTGGAATATGCAGCCACAGTGCTTGAATCTGTGTATATTGATGAAACA
  AGGAGACTCCTGGATACAGAGGATGAGCTCAGTGACATTCAGTCAGATGCTGTGCCTTCTGAGGTCCGAG
  ACTGGCTGGCCTCCACCTTCACGCGGCAGATGGGGATGATGCTCAGGAGGAGCGACGAGAAGCCCCGGTT
  CAAGAGCATCGTTCACGCAGTGCAGGCTGGGATATTTGTGGAGAGAATGTATAGACGGACATCAAACATG
  GTTGGACTGAGCTATCCACCAGCTGTTATTGAGGCATTAAAGGATGTGGACAAGTGGTCCTTTGACGTCT
  TTTCCCTCAATGAGGCCAGTGGGGATCATGCACTGAAATTTATTTTCTATGAACTACTCACACGTTATGA
  TCTGATCAGCCGTTTCAAGATCCCCATTTCTGCACTTGTCTCATTTGTGGAGGCCCTGGAAGTGGGATAC
  AGCAAGCACAAAAATCCTTACCATAACTTAATGCACGCTGCCGATGTTACACAGACAGTGCATTACCTCC
  TCTATAAGACAGGAGTGGCGAACTGGCTGACGGAGCTGGAGATCTTTGCTATAATCTTCTCAGCTGCCAT
  CCATGACTACGAGCATACCGGAACCACCAACAATTTCCACATTCAGACTCGGTCTGATCCAGCTATTCTG
  TATAATGACAGATCTGTACTGGAGAATCACCATTTAAGTGCAGCTTATCGCCTTCTGCAAGATGACGAGG
  AAATGAATATTTTGATTAACCTCTCAAAGGATGACTGGAGGGAGTTTCGAACCTTGGTAATTGAAATGGT
  GATGGCCACAGATATGTCTTGTCACTTCCAACAAATCAAAGCAATGAAGACTGCTCTGCAGCAGCCAGAA
  GCCATTGAAAAGCCAAAAGCCTTATCCCTTATGCTGCATACAGCAGATATTAGCCATCCAGCAAAAGCAT
  GGGACCTCCATCATCGCTGGACAATGTCACTCCTGGAGGAGTTCTTCAGACAGGGTGACAGAGAAGCAGA
  GCTGGGGCTGCCTTTTTCTCCTCTGTGTGACCGAAAGTCCACTATGGTTGCTCAGTCACAAGTAGGTTTC
  ATTGATTTCATCGTGGAACCCACCTTCACTGTGCTTACGGACATGACCGAGAAGATTGTGAGTCCATTAA
  TCGATGAAACCTCTCAAACTGGTGGGACAGGACAGAGGCGTTCGAGTTTGAATAGCATCAGCTCGTCAGA
  TGCCAAGCGATCAGGTGTCAAGACCTCTGGTTCAGAGGGAAGTGCCCCGATCAACAATTCTGTCATCTCC
  GTTGACTATAAGAGCTTTAAAGCTACTTGGACGGAAGTGGTGCACATCAATCGGGAGAGATGGAGGGCCA
  AGGTACCCAAAGAGGAGAAGGCCAAGAAGGAAGCAGAGGAAAAGGCTCGCCTGGCCGCAGAGGAGCAGCA
  AAAGGAAATGGAAGCCAAAAGCCAGGCTGAAGAAGGCGCATCTGGCAAAGCTGAGAAAAAGACGTCTGGA
  GAAACTAAGAATCAAGTCAATGGAACACGGGCAAACAAAAGTGACAACCCTCGTGGGAAAAACTCCAAAG
  CCGAGAAGTCATCAGGAGAACAGCAACAGAATGGTGACTTCAAAGATGGTAAAAATAAGACAGACAAGAA
  GGATCACTCTAACATCGGAAATGATTCAAAGAAAACAGATGGCACAAAACAGCGTTCTCACGGCTCACCA
  GCCCCAAGCACCAGCTCCACGTGTCGCCTTACGTTGCCAGTCATCAAGCCTCCTTTGCGTCATTTTAAAC
  GCCCTGCTTACGCATCTAGCTCCTATGCACCTTCAGTCTCAAAGAAAACTGATGAGCATCCTGCAAGGTA
  CAAGATGCTGGATCAGAGGATCAAAATGAAAAAGATTCAGAACATCTCACATAACTGGAACAGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231408 representing NM_001191058
  Red=Cloning site Green=Tags(s)
  
  MTDAGNRKEGFKKCRSATFSIDGYSFTIVANEAGDKNARPLARFSRSKSQNCLWNSLIDGLTGNVKEKPR
  PTIVHDPRPPEEILADELPQLDSSEVLVKTSFRLRSLVKQLERGEASVVDLKKNLEYAATVLESVYIDET
  RRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNM
  VGLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALVSFVEALEVGY
  SKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAIL
  YNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKAMKTALQQPE
  AIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKSTMVAQSQVGF
  IDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVIS
  VDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEMEAKSQAEEGASGKAEKKTSG
  ETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTDGTKQRSHGSP
  APSTSSTCRLTLPVIKPPLRHFKRPAYASSSYAPSVSKKTDEHPARYKMLDQRIKMKKIQNISHNWNRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191058
• ORF Size: 2307 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191058.1, NM_001191058.2, NM_001191058.3, NP_001177987.1
• RefSeq ORF: 2310 bp
• Locus ID: 5137
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Olfactory transduction, Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 87.4 kDa
• Gene Summary: 'This gene encodes an enzyme that belongs to the 3'5'-cyclic nucleotide phosphodiesterase family. Members of this family catalyze hydrolysis of the cyclic nucleotides, cyclic adenosine monophosphate and cyclic guanosine monophosphate, to the corresponding nucleoside 5'-monophosphates. The enzyme encoded by this gene regulates proliferation and migration of vascular smooth muscle cells, and neointimal hyperplasia. This enzyme also plays a role in pathological vascular remodeling by regulating the stability of growth factor receptors, such as PDGF-receptor-beta. [provided by RefSeq, Jul 2016]'