DCDC1 (NM_020869) Human Tagged ORF Clone

CAT#: RC231457

DCDC5 (Myc-DDK-tagged)-Human doublecortin domain containing 5 (DCDC5)

Product Images

Western blot validation of overexpression lysate (Cat# LY434455) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231457 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCDC1
• Synonyms: DCDC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231457 representing NM_020869
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCCTGTCCTTCCCAGTGGCCAACTTAATGAGGAGTTTGATTGGCCAATACAAGGACTGCTTGTTC
  CGAGCAGTCCTCCCATGAAGAAACCCATCTGTAAGACAACAGAGCCATATGCCCCTGTGCGACTCAGAGT
  TTTGCAGAATGGAGAGAAGAATAAAAACAGATCCGTTACTATCCTTGGTCCAGATATCTCACCTGGACGG
  AAAACGCAGTGCACTGAGATTTTAAATTTACCTTCTGCAGCTCGGAGATTGTACAATGAAAAGGGGAAGG
  AAATATTTGCCTTAAAAGACCTGCAAAGAGATGAACTGGTGTATGTTTCATGTGGAGAACTCTGGATCAA
  TCCTGACCTGTCCATTGCTCAGCAAAAGAAACAAATATTCCTGAGGAACCTAGAATCAGACATTGCCAAA
  ATTCAAATCTTCTGCAGCACACATAAAATAGAAGCTCTTGTTTTAGAAGTCCAAAGTGACATTGTATCTG
  GAAGCAAGCTTGCTGTGCATAAACCTGTAGCAATTTTTGGAGAAGAGAAGCAAGTTACAGAACCGGAAGA
  AAAGCAAATGCAAGAAGATCCTCTAACAACGGAAAATGCTTCCAGTGAAATTCTAGATTCACACGTAAGA
  GCTCATCTTCGAATGAAGGCTTGTCACACACTTCCCAGGTATGCCTGGCAGGAAACTTCACATGACTTTG
  ATGAGGATGACAGTCTTCCAAAGAAAACGGAAAAAGGGCTCTTTGAAAATGTGGAACCACAGAAGAAACA
  CAGCTGTTCACCAAAGCATAGTAAATTGCACAAGCATTGTCATCAGCAGTTCGAATACAGAGATGGGCAG
  ATTATAAGCCATGCTGCTCCTCAGCTAGTCTTGGGGGTTCAAGGTCCCAATCTCCGATCAGGCATGGAGG
  TGGTTTTGGTGGAGAAGAAATCTGATGGTAGTCATCAGCGCTGGATACACCAGGAAGACAGCAGGACCTT
  TCACCTTGTGAGTAACCCTGACCTTGTGCTGGCAGTGTCTATGACCAAGACTAGAAATGAAGTTTGTGGC
  TATCCAGTTATTGTTCAGAAATATAAGCCGTACAACAATGGAGCTGCCAATCAAAAGTGGCATTACATGA
  AAAATATAAAAGCACTTGTGGCCTTTCATAGCACTGCCTTGGATAAGGAAATTACATCAGCAAATTATGC
  TGGTGTCTGTACATCATCTGTGATTAAAGAAGAAAACATTGATCAACCAGGATACTGTTATCTCTCACCT
  GATGGAAAGAGAAAAACTATGCTCTGCTTGGCTTGTGGACAATCCATGAGAACAGAGAAAGGACTGAAAC
  AATTGCTTCCAGGGGTTCCATTCCTCTGTATTTCAGGCACCAAGACTCAGAAGCCCTTCTTACAAGGGCC
  CTTCAAGGTCATCAGTGTGGCTGAGGTTGATTTGTCGTGTGACAAGGCTGAAAAAACTCTAAGTTACTAC
  CAAGCACGTCTATTGTCTTTACGGATGAAGACCTGCACGCAAGCTGCATCTCACAGTGGCATGGCAGCCA
  CACACCAGAAGGCAGTGAAAATAATTGCATACAAAAATGGGGATGGGTATCGTAATGGGAAGTTAATTGT
  GGCTGGAACATTCCCCATGCTTCTTACAGAATGCACGGAACAACTTGGGCTTGCCAGAGCAGCCTCCAAA
  GTATATACCAAAGATGGAACCCCAATCTTTACCTTGCGTGATTTGGTTTTATGGGCTCTAGATGAATCCT
  TTCTCCAGAGAGACTCTGAGAAACAAAAGCAAGATGCAGCTCCTGTTGGAAAAGAACAGATAATTGTTGA
  AAAAAATCCAAGAATGAAAGTGAAAAACAGATTATTTGCAAAATCTGTGACATCCGATAGTTTGGATGGT
  ATAGACAAGTCTTTGCTTACCCTCATCCTCAGAAATCCTATTGCCATCTGGGTGTCTTGTGGTGAACCAT
  TTCTACCTCCAAATGCTTTGCAGAAAGCAGAAAAATTAGAGAAACAGAACTGGCTAAAAAAGGACAGAAT
  TTTGGCTGATCTAGATACCATGAGACACAAAATGAGACAGTTAAAAGGGCGGCGAGTAGCGGCATGTCAG
  CCAGCCACCATGGTTCCTACCAAGAGCCCTGTGCAGCCCGTGGTGGTTGAAGGAGGCTGGACCGAACAGA
  CTCAACAGGAAATTAAACTCATGGAACTTATAAGACATACAGAGGCACACCTTTCTGAAATCCAAGAAAT
  GGAATCCAAAATAAATTTTCCAATTGCAACCAAACGTATAGCAGTCAAGCCGAGCAACCTGTATAAGCAG
  CCCAACACAAAACGAGTGTGGATTTATCTAAATGGAGGCAGACCTGAAGATGGCACTTATGCCTGGGGCA
  AAACTATTTCAGAGCTGCTGCAAGACTGCTCCTCTCGTCTCAAAATGACCCACCCAGCTAGAGCACTGTA
  CACCCCCAGTGGAGAGCCAATTCAGTCCTGGGACGACATAGAGCGAGATATGGTCATCTGTGTGTCTATG
  GGACATGGTTTCAAAACCCCAAAAGAGTTAAAACAACTGATGGAGATCAGAGCAAATTATGCCAGAATCC
  GAAGGCAGCAGGGCCCTCAAGCCACAGACATTGTGGTGTCACCATCCACGAAGCTGCTGTCTCTGGCACA
  TCTCCACAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231457 representing NM_020869
  Red=Cloning site Green=Tags(s)
  
  MWPVLPSGQLNEEFDWPIQGLLVPSSPPMKKPICKTTEPYAPVRLRVLQNGEKNKNRSVTILGPDISPGR
  KTQCTEILNLPSAARRLYNEKGKEIFALKDLQRDELVYVSCGELWINPDLSIAQQKKQIFLRNLESDIAK
  IQIFCSTHKIEALVLEVQSDIVSGSKLAVHKPVAIFGEEKQVTEPEEKQMQEDPLTTENASSEILDSHVR
  AHLRMKACHTLPRYAWQETSHDFDEDDSLPKKTEKGLFENVEPQKKHSCSPKHSKLHKHCHQQFEYRDGQ
  IISHAAPQLVLGVQGPNLRSGMEVVLVEKKSDGSHQRWIHQEDSRTFHLVSNPDLVLAVSMTKTRNEVCG
  YPVIVQKYKPYNNGAANQKWHYMKNIKALVAFHSTALDKEITSANYAGVCTSSVIKEENIDQPGYCYLSP
  DGKRKTMLCLACGQSMRTEKGLKQLLPGVPFLCISGTKTQKPFLQGPFKVISVAEVDLSCDKAEKTLSYY
  QARLLSLRMKTCTQAASHSGMAATHQKAVKIIAYKNGDGYRNGKLIVAGTFPMLLTECTEQLGLARAASK
  VYTKDGTPIFTLRDLVLWALDESFLQRDSEKQKQDAAPVGKEQIIVEKNPRMKVKNRLFAKSVTSDSLDG
  IDKSLLTLILRNPIAIWVSCGEPFLPPNALQKAEKLEKQNWLKKDRILADLDTMRHKMRQLKGRRVAACQ
  PATMVPTKSPVQPVVVEGGWTEQTQQEIKLMELIRHTEAHLSEIQEMESKINFPIATKRIAVKPSNLYKQ
  PNTKRVWIYLNGGRPEDGTYAWGKTISELLQDCSSRLKMTHPARALYTPSGEPIQSWDDIERDMVICVSM
  GHGFKTPKELKQLMEIRANYARIRRQQGPQATDIVVSPSTKLLSLAHLHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020869
• ORF Size: 2670 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020869.3, NP_065920.2
• RefSeq Size: 4773
• RefSeq ORF: 2673
• Locus ID: 341019
• MW: 100.3 kDa
• Gene Summary: This gene encodes a member of the doublecortin family. The protein encoded by this gene is a hydrophilic, intracellular protein. It contains a single doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. This gene is mainly expressed in adult testis. It does not have a mouse homolog. [provided by RefSeq, Sep 2010]