Apc10 (ANAPC10) (NM_001256709) Human Tagged ORF Clone

CAT#: RC232082

ANAPC10 (Myc-DDK tagged) - Homo sapiens anaphase promoting complex subunit 10 (ANAPC10), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANAPC10
• Synonyms: APC10; DOC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232082 representing NM_001256709
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGTGTCAAGGAAACGAATATTTTAAAAATGACTACACCAAACAAGACACCTCCTGGTGCTGACC
  CCAAGCAGTTGGAAAGGACTGGAACAGTACGGGAAATTGGGTCACAAGCTGTTTGGTCACTCTCATCTTG
  CAAACCAGGATTTGGAGTGGATCAGTTACGAGATGACAATCTAGAAACTTATTGGCAATCAGATGGTTCC
  CAGCCTCATTTAGTGAACATCCAATTCAGAAGAAAAACAACAGTGAAGACATTATGTATTTATGCAGACT
  ACAAATCTGATGAAAGCTATACTCCAAGCAAGATCTCAGTCAGAGTAGGAAATAATTTTCACAACCTTCA
  AGAAATTCGGCAACTTGAGTTGGTGGAACCAAGTGGCTGGATTCATGTTCCCTTAACTGACAATCATAAG
  AAGCCAACTCGTACATTCATGATACAGATTGCTGTTCTAGCCAATCACCAGAATGGAAGAGACACCCATA
  TGAGACAAATTAAAATATACACACCAGTAGAAGAGAGCTCCATTGGTAAATTTCCTAGATGTACAACTAT
  AGATTTCATGATGTATCGTTCAATAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232082 representing NM_001256709
  Red=Cloning site Green=Tags(s)
  
  MGGVKETNILKMTTPNKTPPGADPKQLERTGTVREIGSQAVWSLSSCKPGFGVDQLRDDNLETYWQSDGS
  QPHLVNIQFRRKTTVKTLCIYADYKSDESYTPSKISVRVGNNFHNLQEIRQLELVEPSGWIHVPLTDNHK
  KPTRTFMIQIAVLANHQNGRDTHMRQIKIYTPVEESSIGKFPRCTTIDFMMYRSIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256709
• ORF Size: 588 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001256709.1, NP_001243638.1
• RefSeq Size: 1609
• RefSeq ORF: 591
• Locus ID: 10393
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 22.9 kDa
• Gene Summary: ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]). [supplied by OMIM, Feb 2011]