ORAI2 (NM_001271818) Human Tagged ORF Clone
CAT#: RC232297
- TrueORF®
ORAI2 (Myc-DDK tagged) - Homo sapiens ORAI calcium release-activated calcium modulator 2 (ORAI2), transcript variant 3
"NM_001271818" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ORAI2 |
Synonyms | C7orf19; CBCIP2; MEM142B; TMEM142B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC232297 representing NM_001271818
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCTGAGCTTAACGTGCCTATCGACCCCTCTGCTCCTGCCTGCCCTGAGCCAGGCCATAAGGGCA TGGATTACCGGGACTGGGTCCGCCGCAGCTACCTGGAACTGGTCACCTCTAACCACCACTCGGTACAGGC CCTGTCGTGGCGGAAGCTCTACCTGAGCAGGGCCAAGCTGAAGGCCTCCAGCAGGACCTCCGCCCTCCTC TCCGGCTTTGCCATGGTGGCCATGGTGGAGGTGCAGCTGGAGACGCAGTACCAGTACCCGCGGCCGCTGC TGATTGCCTTCAGCGCCTGCACCACGGTGCTGGTGGCCGTGCACCTGTTCGCCCTCCTCATCAGCACCTG CATCCTGCCCAATGTGGAGGCCGTGAGCAACATCCACAACCTGAACTCCATCAGCGAGTCCCCGCATGAG CGCATGCACCCCTACATCGAGCTGGCCTGGGGCTTCTCCACCGTGCTTGGCATCCTACTCTTCCTGGCCG AGGTGGTGCTGCTCTGCTGGATCAAGTTCCTCCCCGTGGATGCCCGGCGCCAGCCTGGCCCCCCACCTGG CCCTGGGAGTCACACGGGCTGGCAGGCCGCCCTGGTGTCCACCATCATCATGGTGCCCGTGGGCCTCATC TTCGTGGTCTTCACCATCCACTTCTACCGCTCCCTGGTGCGCCACAAAACGGAGCGCCACAACCGCGAGA TCGAGGAGCTCCACAAGCTCAAGGTCCAGCTGGACGGGCATGAGCGCAGCCTGCAGGTCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC232297 representing NM_001271818
Red=Cloning site Green=Tags(s) MSAELNVPIDPSAPACPEPGHKGMDYRDWVRRSYLELVTSNHHSVQALSWRKLYLSRAKLKASSRTSALL SGFAMVAMVEVQLETQYQYPRPLLIAFSACTTVLVAVHLFALLISTCILPNVEAVSNIHNLNSISESPHE RMHPYIELAWGFSTVLGILLFLAEVVLLCWIKFLPVDARRQPGPPPGPGSHTGWQAALVSTIIMVPVGLI FVVFTIHFYRSLVRHKTERHNREIEELHKLKVQLDGHERSLQVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001271818 |
ORF Size | 762 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271818.1, NP_001258747.1 |
RefSeq Size | 10811 bp |
RefSeq ORF | 765 bp |
Locus ID | 80228 |
Protein Families | Transmembrane |
MW | 28.6 kDa |
Gene Summary | Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, STIM1. [UniProtKB/Swiss-Prot Function] |
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