C5L2 (C5AR2) (NM_001271750) Human Tagged ORF Clone

CAT#: RC232518

C5AR2 (Myc-DDK tagged) - Homo sapiens complement component 5a receptor 2 (C5AR2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C5AR2
• Synonyms: C5L2; GPF77; GPR77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232518 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGGGGAACGATTCTGTCAGCTACGAGTATGGGGATTACAGCGACCTCTCGGACCGCCCTGTGGACTG
  CCTGGATGGCGCCTGCCTGGCCATCGACCCGCTGCGCGTGGCCCCGCTCCCACTGTATGCCGCCATCTTC
  CTGGTGGGGGTGCCGGGCAATGCCATGGTGGCCTGGGTGGCTGGGAAGGTGGCCCGCCGGAGGGTGGGTG
  CCACCTGGTTGCTCCACCTGGCCGTGGCGGATTTGCTGTGCTGTTTGTCTCTGCCCATCCTGGCAGTGCC
  CATTGCCCGTGGAGGCCACTGGCCGTATGGTGCAGTGGGCTGTCGGGCGCTGCCCTCCATCATCCTGCTG
  ACCATGTATGCCAGCGTCCTGCTCCTGGCAGCTCTCAGTACCGACCTCTGCTTCCTGGCTCTCGGGCCTG
  CCTGGTGGTCTACGGTTCAGCGGGCGTGCGGGGTGCAGGTGGCCTGTGGGGCAGCCTGGACACTGGCCTT
  GCTGCTCACCGTGCCCTCCGCCATCTACCGCCGGCTGCACCAGGAGCACTTCCCAGCCCGGCTGCAGTGT
  GTGGTGGACTACGGCGGCTCCTCCAGCACCGAGAATGCGGTGACTGCCATCCGGTTTCTTTTTGGCTTCC
  TGGGGCCCCTGGTGGCCGTGGTCAGCTGCCACAGTGCCCTCCTGTGCTGGGCAGCCCGACGCTGCCGGCC
  GCTGGGCACAGCCATTGTGGTGGGGTTTTTTGTCTGCTGGGCACCCTACCACCTGCTGGGGCTGGTGCTC
  ACTGTGGCGGCCCCGAACTCCGCACTCCTGGCCAGGGCCCTGCGGGCTGAACCCCTCATCGTGGGCCTTG
  CCCTCGCTCACAGCTGCCTCAATCCCATGCTCTTCCTGTATTTTGGGAGGGCTCAACTCCGCCGGTCACT
  GCCAGCTGCCTGTCACTGGGCCCTGAGGGAGTCCCAGGGCCAGGACGAAAGTGTGGACAGCAAGAAATCC
  ACCAGCCATGACCTGGTCTCGGAGATGGAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232518 protein sequence
  Red=Cloning site Green=Tags(s)
  
  XWGTILSATSMGITATSRTALWTAWMAPAWPSTRCAWPRSHCMPPSSWWGCRAMPWWPGWLGRWPAGGWV
  PPGCSTWPWRICCAVCLCPSWQCPLPVEATGRMVQWAVGRCPPSSC*PCMPASCSWQLSVPTSASWLSGL
  PGGLRFSGRAGCRWPVGQPGHWPCCSPCPPPSTAGCTRSTSQPGCSVWWTTAAPPAPRMR*LPSGFFLAS
  WGPWWPWSAATVPSCAGQPDAAGRWAQPLWWGFLSAGHPTTCWGWCSLWRPRTPHSWPGPCGLNPSSWAL
  PSLTAASIPCSSCILGGLNSAGHCQLPVTGP*GSPRARTKVWTARNPPAMTWSRRWR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271750
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271750.1, NP_001258679.1
• RefSeq Size: 1357 bp
• RefSeq ORF: 1014 bp
• Locus ID: 27202
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 36.1 kDa
• Gene Summary: This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]