SEPTIN5 (NM_001009939) Human Tagged ORF Clone

CAT#: RC232540

SEPT5 (Myc-DDK tagged) - Homo sapiens septin 5 (SEPT5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEPTIN5
• Synonyms: CDCREL; CDCREL-1; CDCREL1; H5; HCDCREL-1; PNUTL1; SEPT5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232540 representing NM_001009939
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGCTGGCAGCGCCCCAGGACCGCCTGGTGGAGCAGCTGCTGTCGCCGCGGACCCAGGCCCAGA
  GGCGGCTCAAGGACATTGACAAGCAGTACGTGGGCTTCGCCACACTGCCCAACCAGGTGCACCGCAAGTC
  GGTGAAGAAAGGCTTTGACTTCACACTCATGGTGGCTGGTGAGTCAGGCCTGGGGAAGTCCACACTGGTC
  CACAGCCTCTTCCTGACAGACTTGTACAAGGACCGGAAGCTGCTCAGTGCTGAGGAGCGCATCAGCCAGA
  CGGTAGAGATTCTAAAACACACGGTGGACATTGAGGAGAAGGGAGTCAAGCTGAAGCTCACCATCGTGGA
  CACGCCGGGATTCGGGGACGCTGTCAACAACACCGAGTGCTGGAAGCCCATCACCGACTATGTGGACCAG
  CAGTTTGAGCAGTACTTCCGTGATGAGAGCGGCCTCAACCGAAAGAACATCCAAGACAACCGAGTGCACT
  GCTGCCTATACTTCATCTCCCCCTTCGGGCATGGGCTGCGGCCAGTGGATGTGGGTTTCATGAAGGCATT
  GCATGAGAAGGTCAACATCGTGCCTCTCATCGCCAAAGCTGACTGTCTTGTCCCCAGTGAGATCCGGAAG
  CTGAAGGAGCGGATCCGGGAGGAGATTGACAAGTTTGGGATCCATGTATACCAGTTCCCTGAGTGTGACT
  CGGACGAGGATGAGGACTTCAAGCAGCAGGACCGGGAACTGAAGGAGAGCGCGCCCTTCGCCGTTATAGG
  CAGCAACACGGTGGTGGAGGCCAAGGGGCAGCGGGTCCGGGGCCGACTGTACCCCTGGGGGATCGTGGAG
  GGCGCATTGCGACTTCGTGAAGCTGCGCAACATGCTCATCCGCACGCATATGCACGACCTCAAGGACGTG
  ACGTGCGACGTGCACTACGAGAACTACCGCGCGCACTGCATCCAGCAGATGACCAGCAAACTGACCCAGG
  ACAGCCGCATGGAGAGCCCCATCCCGATCCTGCCGCTGCCCACCCCGGACGCCGAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232540 representing NM_001009939
  Red=Cloning site Green=Tags(s)
  
  MDSLAAPQDRLVEQLLSPRTQAQRRLKDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLV
  HSLFLTDLYKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQ
  QFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRK
  LKERIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVE
  GALRLREAAQHAHPHAYARPQGRDVRRALRELPRALHPADDQQTDPGQPHGEPHPDPAAAHPGRRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009939
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009939.1, NM_001009939.2, NP_001009939.1
• RefSeq Size: 2284 bp
• RefSeq ORF: 1041 bp
• Locus ID: 5413
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease
• MW: 39.8 kDa
• Gene Summary: 'This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. [provided by RefSeq, Dec 2010]'