BORIS (CTCFL) (NM_001269052) Human Tagged ORF Clone

CAT#: RC232667

CTCFL (Myc-DDK tagged) - Homo sapiens CCCTC-binding factor (zinc finger protein)-like (CTCFL), transcript variant 14

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CTCFL
• Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232667 representing NM_001269052
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCACTGAGATCTCTGTCCTTTCTGAGCAATTCACCAAGATCAAAGAACTCGAGTTGATGCCGG
  AAAAAGGCCTGAAGGAGGAGGAAAAAGACGGAGTGTGCAGAGAGAAAGACCATCGGAGCCCTAGTGAGTT
  GGAGGCCGAGCGTACCTCTGGGGCCTTCCAGGACAGCGTCCTGGAGGAAGAAGTGGAGCTGGTGCTGGCC
  CCCTCGGAGGAGAGCGAGAAGTACATCCTGACCCTGCAGACGGTGCACTTCACTTCTGAAGCTGTGGAGT
  TGCAGGATATGAGCTTGCTGAGCATACAGCAGCAAGAAGGGGTGCAGGTGGTGGTGCAACAGCCTGGCCC
  TGGGTTGCTGTGGCTTGAGGAAGGGCCCCGGCAGAGCCTGCAGCAGTGTGTGGCCATTAGTATCCAGCAA
  GAGCTGTACTCCCCGCAAGAGATGGAGGTGTTGCAGTTCCACGCTCTAGAGGAGAATGTGATGGTGGCCA
  GTGAAGACAGTAAGTTAGCGGTGAGCCTGGCTGAAACTACTGGACTGATCAAGCTCGAGGAAGAGCAGGA
  GAAGAACCAGTTATTGGCTGAAAGAACAAAGGAGCAGCTCTTTTTTGTGGAAACAATGTCAGGAGATGAA
  AGAAGTGACGAAATTGTTCTCACAGTTTCAAATTCAAATGTGGAAGAACAAGAGGATCAACCTACAGCTG
  GTCAAGCAGATGCTGAAAAGGCCAAATCTACAAAAAATCAAAGAAAGACAAAGGGAGCAAAAGGAACCTT
  CCACTGTGATGTCTGCATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATGAAAACTCACACC
  AGTGAGAAGCCTCACCTGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTGCTGCGGAACCATG
  TTAACACCCACACAGGAACCAGGCCCTACAAGTGTAACGACTGCAACATGGCATTTGTCACCAGTGGAGA
  ACTCGTCCGACACAGGCGCTATAAACATACTCATGAGAAACCCTTTAAATGTTCCATGTGCAAGTATGCC
  AGTGTGGAGGTAAAGCCATTCTTGGACTTGAAGCTTCATGGCATCTTAGTAGAGGCTGCTGTACAAGTTA
  CTCCAAGTGTAACTAACAGTAGAATCTGTTACAAACAGGCTTTTTATTATTCATATAAAATTTATGCAGG
  AAATAATATGCATTCTCTTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232667 representing NM_001269052
  Red=Cloning site Green=Tags(s)
  
  MAATEISVLSEQFTKIKELELMPEKGLKEEEKDGVCREKDHRSPSELEAERTSGAFQDSVLEEEVELVLA
  PSEESEKYILTLQTVHFTSEAVELQDMSLLSIQQQEGVQVVVQQPGPGLLWLEEGPRQSLQQCVAISIQQ
  ELYSPQEMEVLQFHALEENVMVASEDSKLAVSLAETTGLIKLEEEQEKNQLLAERTKEQLFFVETMSGDE
  RSDEIVLTVSNSNVEEQEDQPTAGQADAEKAKSTKNQRKTKGAKGTFHCDVCMFTSSRMSSFNRHMKTHT
  SEKPHLCHLCLKTFRTVTLLRNHVNTHTGTRPYKCNDCNMAFVTSGELVRHRRYKHTHEKPFKCSMCKYA
  SVEVKPFLDLKLHGILVEAAVQVTPSVTNSRICYKQAFYYSYKIYAGNNMHSLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001269052
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001269052.1, NP_001255981.1
• RefSeq Size: 1985
• RefSeq ORF: 1215
• Locus ID: 140690
• Protein Families: Transcription Factors
• MW: 46.3 kDa
• Gene Summary: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]