MCT2 (SLC16A7) (NM_001270623) Human Tagged ORF Clone

CAT#: RC232821

SLC16A7 (Myc-DDK tagged) - Homo sapiens solute carrier family 16 (monocarboxylate transporter), member 7 (SLC16A7), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC16A7
• Synonyms: MCT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232821 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCAATGCCAAGTGCCCCACCTGTGCATCCACCTCCAGATGGAGGATGGGGTTGGATTGTGGTTG
  GAGCAGCTTTTATCTCCATTGGATTTTCCTATGCATTCCCCAAAGCTGTCACCGTATTCTTCAAAGAAAT
  TCAGCAAATATTCCACACTACCTACAGTGAAATAGCATGGATTTCATCCATTATGCTGGCTGTTATGTAC
  GCAGGAGGTCCTGTAAGTAGTGTTTTGGTGAATAAATACGGCAGCCGGCCGGTGGTGATAGCAGGAGGCT
  TATTATGCTGTCTTGGAATGGTGTTGGCCTCCTTTAGTAGCAGCGTGGTACAGCTGTACCTCACTATGGG
  ATTCATTACAGGTTTAGGTTTAGCCTTCAACCTGCAACCCGCCTTAACCATAATTGGCAAATACTTCTAT
  AGGAAGCGACCCATGGCAAATGGATTGGCCATGGCAGGAAGTCCTGTTTTCTTAAGTTCATTGGCTCCTT
  TCAATCAGTACCTTTTTAATACTTTTGGCTGGAAAGGAAGCTTCCTGATTTTGGGAAGTCTACTTTTGAA
  TGCCTGTGTGGCTGGTTCCCTCATGAGACCCCTTGGACCCAATCAAACCACTTCTAAGTCTAAAAATAAG
  ACTGGCAAAACAGAAGATGATTCAAGCCCAAAGAAAATCAAAACGAAGAAATCAACTTGGGAAAAAGTTA
  ATAAGTATTTAGATTTCTCCCTTTTTAAGCATAGAGGATTTCTGATATATCTGTCTGGAAATGTCATTAT
  GTTCCTAGGTTTTTTTGCCCCCATTATATTCTTGGCTCCATATGCTAAAGACCAAGGAATTGATGAGTAC
  TCGGCAGCTTTTCTGCTATCTGTTATGGCTTTCGTTGATATGTTTGCTAGGCCTTCTGTAGGATTAATTG
  CAAACTCCAAATATATTCGACCTCGAATTCAGTACTTCTTCAGTTTTGCAATCATGTTCAATGGAGTGTG
  TCACCTCTTGTGCCCACTGGCACAGGACTACACAAGCCTGGTATTATATGCTGTATTTTTTGGCCTTGGA
  TTTGGGAGTGTTAGCAGTGTTCTCTTTGAAACTCTCATGGACCTCGTGGGTGCACCAAGATTTTCCAGTG
  CCGTCGGACTTGTCACAATTGTGGAGTGTGGCCCAGTTCTTCTTGGCCCTCCTCTTGCAGGTAAATTGGT
  GGATTTAACTGGAGAATATAAATACATGTACATGTCCTGTGGGGCTATTGTGGTAGCAGCAAGCGTGTGG
  CTGCTCATTGGCAATGCTATCAACTATAGATTGCTTGCAAAGGAAAGGAAGGAGGAAAATGCAAGGCAGA
  AGACCAGAGAATCTGAACCCTTGAGCAAATCTAAACATTCGGAAGATGTTAACGTCAAAGTTTCAAATGC
  ACAGAGTGTAACCTCAGAAAGAGAAACTAACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232821 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPPMPSAPPVHPPPDGGWGWIVVGAAFISIGFSYAFPKAVTVFFKEIQQIFHTTYSEIAWISSIMLAVMY
  AGGPVSSVLVNKYGSRPVVIAGGLLCCLGMVLASFSSSVVQLYLTMGFITGLGLAFNLQPALTIIGKYFY
  RKRPMANGLAMAGSPVFLSSLAPFNQYLFNTFGWKGSFLILGSLLLNACVAGSLMRPLGPNQTTSKSKNK
  TGKTEDDSSPKKIKTKKSTWEKVNKYLDFSLFKHRGFLIYLSGNVIMFLGFFAPIIFLAPYAKDQGIDEY
  SAAFLLSVMAFVDMFARPSVGLIANSKYIRPRIQYFFSFAIMFNGVCHLLCPLAQDYTSLVLYAVFFGLG
  FGSVSSVLFETLMDLVGAPRFSSAVGLVTIVECGPVLLGPPLAGKLVDLTGEYKYMYMSCGAIVVAASVW
  LLIGNAINYRLLAKERKEENARQKTRESEPLSKSKHSEDVNVKVSNAQSVTSERETNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270623
• ORF Size: 1434 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270623.1, NP_001257552.1
• RefSeq Size: 11939 bp
• RefSeq ORF: 1437 bp
• Locus ID: 9194
• Protein Families: Transmembrane
• MW: 52.2 kDa
• Gene Summary: This gene is a member of the monocarboxylate transporter family. Members in this family transport metabolites, such as lactate, pyruvate, and ketone bodies. The protein encoded by this gene catalyzes the proton-linked transport of monocarboxylates and has the highest affinity for pyruvate. This protein has been reported to be more highly expressed in prostate and colorectal cancer specimens when compared to control specimens. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]