ELF2 (NM_001276457) Human Tagged ORF Clone

CAT#: RC232880

ELF2 (Myc-DDK tagged) - Homo sapiens E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELF2
• Synonyms: b; EU32; NERF; NERF-1A; NERF-1a; NERF-1B; NERF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232880 representing NM_001276457
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCAG
  TTCACAGCAGTAATGCACACTGTACAGATAAGACAATTGAAGCTGCTGAAGCCCTGCTTCATATGGAATC
  TCCTACCTGCTTGAGGGATTCAAGAAGTCCTGTGGAAGTGTTTGTTCCTCCTTGTGTATCAACTCCAGAA
  TTCATCCATGCTGCTATGAGGCCAGATGTCATTACAGAAACTGTAGTGGAGGTGTCAACTGAAGAGTCTG
  AACCCATGGATACCTCTCCTATTCCAACATCACCAGATAGCCATGAACCAATGAAAAAGAAAAAAGGAAA
  CACAACCTATTTGTGGGAGTTTCTTTTAGATCTACTTCAAGATAAAAATACTTGTCCCAGGTATATTAAA
  TGGACTCAGAGAGAAAAAGGCATATTCAAGCTGGTGGATTCAAAGGCTGTCTCTAAGCTTTGGGGAAAGC
  ATAAGAACAAACCAGACATGAACTATGAAACCATGGGACGAGCTTTGAGATACTACTACCAAAGGGGAAT
  TCTTGCAAAGGTTGAAGGACAGAGGCTTGTATATCAGTTCAAGGATATGCCGAAAAACATAGTGGTCATA
  GATGATGACAAAAGTGAAACCTGTAATGAAGATTTAGCAGGAACTACTGATGAAAAATCATTAGAACGAG
  TGTCACTGTCTGCAGAAAGTCTCCTGAAAGCAGCATCCTCTGTTCGCAGTGGAAAAAATTCATCCCCTAT
  AAACTGCTCCAGAGCAGAGAAGGGTGTAGCTAGAGTTGTGAATATCACTTCCCCTGGGCACGATGCTTCA
  TCCAGGTCTCCTACTACCACTGCATCTGTGTCAGCAACAGCAGCTCCAAGGACAGTTCGTGTGGCAATGC
  AGGTACCTGTTGTAATGACATCATTGGGTCAGAAAATTTCAACTGTGGCAGTTCAGTCAGTTAATGCAGG
  TGCACCATTAATAACCAGCACTAGTCCAACAACAGCGACCTCTCCAAAGGTAGTCATTCAGACAATCCCT
  ACTGTGATGCCAGCTTCTACTGAAAATGGAGACAAAATCACCATGCAGCCTGCCAAAATTATTACCATCC
  CAGCTACACAGCTTGCACAGTGTCAACTGCAGACAAAGTCAAATCTGACTGGATCAGGAAGCATTAACAT
  TGTTGGAACCCCATTGGCTGTGAGAGCACTTACCCCTGTTTCAATAGCCCATGGTACACCTGTAATGAGA
  CTATCAATGCCTACTCAGCAGGCATCTGGCCAGACTCCTCCTCGAGTTATCAGTGCAGTCATAAAGGGGC
  CAGAGGTTAAATCGGAAGCAGTGGCAAAAAAGCAAGAACATGATGTGAAAACTTTGCAGCTAGTAGAAGA
  AAAACCAGCAGATGGAAATAAGACAGTGACCCACGTAGTGGTTGTCAGTGCGCCTTCAGCTATTGCCCTT
  CCTGTAACTATGAAAACAGAAGGACTAGTGACATGTGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232880 representing NM_001276457
  Red=Cloning site Green=Tags(s)
  
  MATSLHEGPTNQLDLLIRAVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPVEVFVPPCVSTPE
  FIHAAMRPDVITETVVEVSTEESEPMDTSPIPTSPDSHEPMKKKKGNTTYLWEFLLDLLQDKNTCPRYIK
  WTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKDMPKNIVVI
  DDDKSETCNEDLAGTTDEKSLERVSLSAESLLKAASSVRSGKNSSPINCSRAEKGVARVVNITSPGHDAS
  SRSPTTTASVSATAAPRTVRVAMQVPVVMTSLGQKISTVAVQSVNAGAPLITSTSPTTATSPKVVIQTIP
  TVMPASTENGDKITMQPAKIITIPATQLAQCQLQTKSNLTGSGSINIVGTPLAVRALTPVSIAHGTPVMR
  LSMPTQQASGQTPPRVISAVIKGPEVKSEAVAKKQEHDVKTLQLVEEKPADGNKTVTHVVVVSAPSAIAL
  PVTMKTEGLVTCEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276457
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276457.1, NP_001263386.1
• RefSeq Size: 3005 bp
• RefSeq ORF: 1515 bp
• Locus ID: 1998
• Cytogenetics: 4q31.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 54.7 kDa
• Gene Summary: ''