C8ORF41 (TTI2) (NM_001265581) Human Tagged ORF Clone

CAT#: RC232890

TTI2 (Myc-DDK tagged) - Homo sapiens TELO2 interacting protein 2 (TTI2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TTI2
• Synonyms: C8orf41; MRT39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232890 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTTGACAGCGCTCTGGAAGCCCCATCGCAGGAAGACTCTAATTTGTCCGAGGAGTTGTCTCACT
  CCGCCTTTGGACAGGCCTTCTCCAAGATTTTACACTGTCTTGCCCGCCCGGAGGCACGACGAGGCAATGT
  AAAAGATGCAGTTCTTAAAGACCTCGGTGATCTAATAGAAGCCACAGAATTTGATAGGTTATTTGAGGGG
  ACTGGTGCACGGCTCCGCGGAATGCCGGAGACACTGGGGCAGGTAGCAAAAGCCCTGGAGAAGTATGCAG
  CCCCCTCCAAGGAGGAGGAAGGTGGAGGTGATGGGCACTCCGAAGCGGCCGAGAAAGCAGCCCAAGTTGG
  GTTACTGTTTCTTAAACTGTTAGGGAAAGTTGAGACTGCTAAGAATTCCCTGGTCGGCCCTGCATGGCAG
  ACGGGCCTGCATCACTTGGCAGGACCCGTTTATATTTTTGCCATCACACACAGCTTGGAGCAACCATGGA
  CCACTCCGAGATCTCGGGAAGTTGCTAGGGAGGTGCTCACCTCACTGCTTCAAGTTACTGAATGCGGTTC
  TGTGGCAGGATTCCTACATGGAGAAAATGAAGATGAGAAAGGGAGACTTTCGGTGATACTAGGGCTTCTC
  AAACCCGACTTGTATAAGGAATCCTGGAAGAATAACCCTGCCATCAAACATGTTTTCTCATGGACTCTGC
  AACAGGTCACTCGGCCCTGGCTGAGCCAGCATCTGGAAAGGGTACTTCCCGCATCATTGGTCATTTCAGA
  TGACTATCAGACTGAGAACAAAATCCTGGGTGTACACTGTCTCCATCACATTGTGCTTAATGTGCCAGCT
  GCTGATTTGCTCCAGTATAACAGAGCCCAGGTCCTATACCATGCCATTTCCAACCACCTGTACACACCAG
  AGCACCACCTCATTCAGGCTGTGCTCCTGTGTCTGCTGGATTTATTCCCCATCCTGGAGAAAACCCTGCA
  CTGGAAAGGAGATGGAGCTCGACCCACCACCCATTGTGATGAGGTCCTGCGGCTGATCCTGACCCACATG
  GAGCCAGAGCACCGCCTTCTTTTACGCAGGACCTACGCAAGAAACCTGCCGGCTTTCGTGAACAGGTTGG
  GGATCCTAACTGTCCGGCACTTAAAGAGGCTGGAGAGAGTCATCATTGGTTATCTGGAGGTTTATGATGG
  ACCTGAGGAGGAAGCTAGACTGAAGATATTGGAAACCCTAAAACTTCTCATGCAACATACTTGGCCCAGA
  GTTTCCTGCAGACTTGTGGTCTTACTGAAGGCCCTCTTGAAACTGATTTGTGATGTAGCAAGGGATCCAA
  ACCTTACACCTGAGTCTGTTAAGAGCGCCCTGCTACAGGAGGCCACAGACTGCCTGATTCTCCTGGACCG
  CTGTTCTCAAGGACGGGTAAAGGGTCTCCTGGCCAAAATTCCCCAAAGCTGTGAAGACAGAAAAGTGGTG
  AACTATATCAGAAAAGTGCAGCAGGTTTCTGAAGGCGCACCCTACAATGGAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232890 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELDSALEAPSQEDSNLSEELSHSAFGQAFSKILHCLARPEARRGNVKDAVLKDLGDLIEATEFDRLFEG
  TGARLRGMPETLGQVAKALEKYAAPSKEEEGGGDGHSEAAEKAAQVGLLFLKLLGKVETAKNSLVGPAWQ
  TGLHHLAGPVYIFAITHSLEQPWTTPRSREVAREVLTSLLQVTECGSVAGFLHGENEDEKGRLSVILGLL
  KPDLYKESWKNNPAIKHVFSWTLQQVTRPWLSQHLERVLPASLVISDDYQTENKILGVHCLHHIVLNVPA
  ADLLQYNRAQVLYHAISNHLYTPEHHLIQAVLLCLLDLFPILEKTLHWKGDGARPTTHCDEVLRLILTHM
  EPEHRLLLRRTYARNLPAFVNRLGILTVRHLKRLERVIIGYLEVYDGPEEEARLKILETLKLLMQHTWPR
  VSCRLVVLLKALLKLICDVARDPNLTPESVKSALLQEATDCLILLDRCSQGRVKGLLAKIPQSCEDRKVV
  NYIRKVQQVSEGAPYNGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001265581
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001265581.1, NP_001252510.1
• RefSeq Size: 2516 bp
• RefSeq ORF: 1527 bp
• Locus ID: 80185
• MW: 56.9 kDa
• Gene Summary: This gene encodes a regulator of the DNA damage response. The protein is a component of the Triple T complex (TTT) which also includes telomere length regulation protein and TELO2 interacting protein 1. The TTT complex is involved in cellular resistance to DNA damage stresses and may act as a regulator of phosphoinositide-3-kinase-related protein kinase (PIKK) abundance. [provided by RefSeq, May 2013]