ARSF (NM_001201539) Human Tagged ORF Clone
CAT#: RC232997
- TrueORF®
ARSF (Myc-DDK tagged) - Homo sapiens arylsulfatase F (ARSF), transcript variant 3
"NM_001201539" in other vectors (2)
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Other products for "ARSF"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ARSF |
Synonyms | ASF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC232997 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC RCATGAGGCCCAGGAGACCCTTGGTCTTCATGTCTTTGGTGTGTGCACTCTTGAACACATGCCAGGCACA CAGGGTGCATGACGACAAGCCTAATATTGTCCTAATCATGGTTGATGACCTGGGTATTGGAGATCTGGGC TGCTACGGCAATGACACCATGAGGACGCCTCACATCGACCGCCTTGCCAGGGAAGGCGTGCGACTGACTC AGCACATCTCTGCCGCCTCCCTCTGCAGCCCAAGCCGGTCCGCGTTCTTGACGGGAAGATACCCCATCCG ATCAGGTATGGTTTCTAGTGGTAATAGACGTGTCATCCAAAATCTTGCAGTCCCCGCAGGCCTCCCTCTT AATGAGACAACACTTGCAGCCTTGCTAAAGAAGCAAGGATACAGCACGGGGCTTATAGGCAAATGGCACC AAGGCTTGAACTGCGACTCCCGAAGTGACCAGTGCCACCATCCATATAATTATGGGTTTGACTACTACTA TGGCATGCCGTTCACTCTCGTTGACAGCTGCTGGCCGGACCCCTCTCGTAACACGGAATTAGCCTTTGAG AGTCAGCTCTGGCTCTGTGTGCAGCTAGTTGCCATTGCCATCCTCACCCTAACCTTTGGGAAGCTGAGCG GCTGGGTCTCTGTTCCCTGGCTCCTGATCTTCTCCATGATTCTGTTTATTTTCCTCTTGGGCTATGCTTG GTTCTCCAGCCACACGTCCCCTTTATACTGGGACTGCCTCCTCATGCGGGGGCACGAGATCACGGAGCAG CCCATGAAGGCTGAACGAGCTGGATCCATTATGGTGAAGGAAGCGATTTCCTTTTTAGAAAGGCACAGTA AGGAAACTTTCCTTCTCTTTTTCTCCTTTCTTCACGTGCACACACCTCTCCCCACCACGGACGATTTCAC TGGCACCAGCAAGCATGGCTTGTATGGGGATAATGTGGAAGAGATGGACTCCATGGTGGGCAAGATTCTT GATGCTATCGATGATTTTGGCCTAAGGAACAACACCCTTGTCTACTTTACATCAGATCACGGAGGGCATT TGGAAGCTAGGCGAGGGCATGCCCAACTTGGTGGATGGAATGGAATATACAAAGGTGGAAAAGGCATGGG GGGCTGGGAAGGTGGAATCCGCGTCCCAGGAATTGTCCGATGGCCTGGAAAGGTACCAGCTGGACGGTTG ATTAAGGAACCTACAAGTTTAATGGATATTTTACCAACTGTCGCATCAGTGTCAGGAGGAAGTCTCCCTC AGGACAGGGTCATTGACGGCCGAGACCTCATGCCCTTGCTGCAGGGCAACGTCAGGCACTCGGAGCATGA ATTTCTTTTCCACTACTGTGGCTCCTACCTGCACGCCGTGCGGTGGATCCCCAAGGACGACAGTGGGTCA GTTTGGAAGGCTCACTATGTGACCCCGGTATTCCAGCCACCAGCTTCTGGTGGCTGCTATGTCACCTCAT TATGCAGATGTTTCGGAGAACAGGTTACCTACCACAACCCCCCTCTGCTCTTCGATCTCTCCAGGGACCC CTCAGAGTCCACACCCCTGACACCTGCCACAGAGCCCCTCTATGATTTTGTGATTAAAAAGGTGGCCAAC GCCCTGAAGGAACACCAGGAAACCATCGTGCCTGTGACCTACCAACTCTCAGAACTGAATCAGGGCAGGA CGTGGCTGAAGCCTTGCTGTGGGGTGTTCCCATTTTGTCTGTGTGACAAGGAAGAGGAAGTCTCTCAGCC TCGGGGTCCTAACGAGAAGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC232997 protein sequence
Red=Cloning site Green=Tags(s) X*GPGDPWSSCLWCVHS*THARHTGCMTTSLILS*SWLMTWVLEIWAATAMTP*GRLTSTALPGKACD*L STSLPPPSAAQAGPRS*REDTPSDQVWFLVVIDVSSKILQSPQASLLMRQHLQPC*RSKDTARGL*ANGT KA*TATPEVTSATIHIIMGLTTTMACRSLSLTAAGRTPLVTRN*PLRVSSGSVCS*LPLPSSP*PLGS*A AGSLFPGS*SSP*FCLFSSWAMLGSPATRPLYTGTASSCGGTRSRSSP*RLNELDPLW*RKRFPF*KGTV RKLSFSFSPFFTCTHLSPPRTISLAPASMACMGIMWKRWTPWWARFLMLSMILA*GTTPLSTLHQITEGI WKLGEGMPNLVDGMEYTKVEKAWGAGKVESASQELSDGLERYQLDG*LRNLQV*WIFYQLSHQCQEEVSL RTGSLTAETSCPCCRATSGTRSMNFFSTTVAPTCTPCGGSPRTTVGQFGRLTM*PRYSSHQLLVAAMSPH YADVSENRLPTTTPLCSSISPGTPQSPHP*HLPQSPSMIL*LKRWPTP*RNTRKPSCL*PTNSQN*IRAG RG*SLAVGCSHFVCVTRKRKSLSLGVLTRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001201539 |
ORF Size | 1770 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001201539.1, NP_001188468.1 |
RefSeq Size | 2167 bp |
RefSeq ORF | 1773 bp |
Locus ID | 416 |
Cytogenetics | Xp22.33 |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
MW | 66 kDa |
Gene Summary | 'This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]' |
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