ARSF (NM_001201539) Human Tagged ORF Clone

CAT#: RC232997

ARSF (Myc-DDK tagged) - Homo sapiens arylsulfatase F (ARSF), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARSF
• Synonyms: ASF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232997 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGAGGCCCAGGAGACCCTTGGTCTTCATGTCTTTGGTGTGTGCACTCTTGAACACATGCCAGGCACA
  CAGGGTGCATGACGACAAGCCTAATATTGTCCTAATCATGGTTGATGACCTGGGTATTGGAGATCTGGGC
  TGCTACGGCAATGACACCATGAGGACGCCTCACATCGACCGCCTTGCCAGGGAAGGCGTGCGACTGACTC
  AGCACATCTCTGCCGCCTCCCTCTGCAGCCCAAGCCGGTCCGCGTTCTTGACGGGAAGATACCCCATCCG
  ATCAGGTATGGTTTCTAGTGGTAATAGACGTGTCATCCAAAATCTTGCAGTCCCCGCAGGCCTCCCTCTT
  AATGAGACAACACTTGCAGCCTTGCTAAAGAAGCAAGGATACAGCACGGGGCTTATAGGCAAATGGCACC
  AAGGCTTGAACTGCGACTCCCGAAGTGACCAGTGCCACCATCCATATAATTATGGGTTTGACTACTACTA
  TGGCATGCCGTTCACTCTCGTTGACAGCTGCTGGCCGGACCCCTCTCGTAACACGGAATTAGCCTTTGAG
  AGTCAGCTCTGGCTCTGTGTGCAGCTAGTTGCCATTGCCATCCTCACCCTAACCTTTGGGAAGCTGAGCG
  GCTGGGTCTCTGTTCCCTGGCTCCTGATCTTCTCCATGATTCTGTTTATTTTCCTCTTGGGCTATGCTTG
  GTTCTCCAGCCACACGTCCCCTTTATACTGGGACTGCCTCCTCATGCGGGGGCACGAGATCACGGAGCAG
  CCCATGAAGGCTGAACGAGCTGGATCCATTATGGTGAAGGAAGCGATTTCCTTTTTAGAAAGGCACAGTA
  AGGAAACTTTCCTTCTCTTTTTCTCCTTTCTTCACGTGCACACACCTCTCCCCACCACGGACGATTTCAC
  TGGCACCAGCAAGCATGGCTTGTATGGGGATAATGTGGAAGAGATGGACTCCATGGTGGGCAAGATTCTT
  GATGCTATCGATGATTTTGGCCTAAGGAACAACACCCTTGTCTACTTTACATCAGATCACGGAGGGCATT
  TGGAAGCTAGGCGAGGGCATGCCCAACTTGGTGGATGGAATGGAATATACAAAGGTGGAAAAGGCATGGG
  GGGCTGGGAAGGTGGAATCCGCGTCCCAGGAATTGTCCGATGGCCTGGAAAGGTACCAGCTGGACGGTTG
  ATTAAGGAACCTACAAGTTTAATGGATATTTTACCAACTGTCGCATCAGTGTCAGGAGGAAGTCTCCCTC
  AGGACAGGGTCATTGACGGCCGAGACCTCATGCCCTTGCTGCAGGGCAACGTCAGGCACTCGGAGCATGA
  ATTTCTTTTCCACTACTGTGGCTCCTACCTGCACGCCGTGCGGTGGATCCCCAAGGACGACAGTGGGTCA
  GTTTGGAAGGCTCACTATGTGACCCCGGTATTCCAGCCACCAGCTTCTGGTGGCTGCTATGTCACCTCAT
  TATGCAGATGTTTCGGAGAACAGGTTACCTACCACAACCCCCCTCTGCTCTTCGATCTCTCCAGGGACCC
  CTCAGAGTCCACACCCCTGACACCTGCCACAGAGCCCCTCTATGATTTTGTGATTAAAAAGGTGGCCAAC
  GCCCTGAAGGAACACCAGGAAACCATCGTGCCTGTGACCTACCAACTCTCAGAACTGAATCAGGGCAGGA
  CGTGGCTGAAGCCTTGCTGTGGGGTGTTCCCATTTTGTCTGTGTGACAAGGAAGAGGAAGTCTCTCAGCC
  TCGGGGTCCTAACGAGAAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232997 protein sequence
  Red=Cloning site Green=Tags(s)
  
  X*GPGDPWSSCLWCVHS*THARHTGCMTTSLILS*SWLMTWVLEIWAATAMTP*GRLTSTALPGKACD*L
  STSLPPPSAAQAGPRS*REDTPSDQVWFLVVIDVSSKILQSPQASLLMRQHLQPC*RSKDTARGL*ANGT
  KA*TATPEVTSATIHIIMGLTTTMACRSLSLTAAGRTPLVTRN*PLRVSSGSVCS*LPLPSSP*PLGS*A
  AGSLFPGS*SSP*FCLFSSWAMLGSPATRPLYTGTASSCGGTRSRSSP*RLNELDPLW*RKRFPF*KGTV
  RKLSFSFSPFFTCTHLSPPRTISLAPASMACMGIMWKRWTPWWARFLMLSMILA*GTTPLSTLHQITEGI
  WKLGEGMPNLVDGMEYTKVEKAWGAGKVESASQELSDGLERYQLDG*LRNLQV*WIFYQLSHQCQEEVSL
  RTGSLTAETSCPCCRATSGTRSMNFFSTTVAPTCTPCGGSPRTTVGQFGRLTM*PRYSSHQLLVAAMSPH
  YADVSENRLPTTTPLCSSISPGTPQSPHP*HLPQSPSMIL*LKRWPTP*RNTRKPSCL*PTNSQN*IRAG
  RG*SLAVGCSHFVCVTRKRKSLSLGVLTRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001201539
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001201539.1, NP_001188468.1
• RefSeq Size: 2167 bp
• RefSeq ORF: 1773 bp
• Locus ID: 416
• Cytogenetics: Xp22.33
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 66 kDa
• Gene Summary: 'This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]'