Contactin 1 (CNTN1) (NM_001256064) Human Tagged ORF Clone

CAT#: RC233027

CNTN1 (Myc-DDK tagged) - Homo sapiens contactin 1 (CNTN1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNTN1
• Synonyms: F3; GP135; MYPCN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233027 representing NM_001256064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGTGGTTGCTGGTCAGTCATCTTGTGATAATATCTATTACTACCTGTTTAGCAGAGTTTACAT
  GGTATAGAAGATATGGTCATGGAGTTTCTGAGGAAGACAAAGGATTTGGACCAATTTTTGAAGAGCAGCC
  AATCAATACCATTTATCCAGAGGAATCACTGGAAGGAAAAGTCTCACTCAACTGTAGGGCACGAGCCAGC
  CCTTTCCCGGTTTACAAATGGAGAATGAATAATGGGGACGTTGATCTCACAAGTGATCGATACAGTATGG
  TAGGAGGAAACCTTGTTATCAACAACCCTGACAAACAGAAAGATGCTGGAATATACTACTGTTTAGCATC
  TAATAACTACGGGATGGTCAGAAGCACTGAAGCAACCCTGAGCTTTGGATATCTTGATCCTTTCCCACCT
  GAGGAACGTCCTGAGGTCAGAGTAAAAGAAGGGAAAGGAATGGTGCTTCTCTGTGACCCCCCATACCATT
  TTCCAGATGATCTTAGCTATCGCTGGCTTCTAAATGAATTTCCTGTATTTATCACAATGGATAAACGGCG
  ATTTGTGTCTCAGACAAATGGCAATCTCTACATTGCAAATGTTGAGGCTTCCGACAAAGGCAATTATTCC
  TGCTTTGTTTCCAGTCCTTCTATTACAAAGAGCGTGTTCAGCAAATTCATCCCACTCATTCCAATACCTG
  AACGAACAACAAAACCATATCCTGCTGATATTGTAGTTCAGTTCAAGGATGTATATGCATTGATGGGCCA
  AAATGTGACCTTAGAATGTTTTGCACTTGGAAATCCTGTTCCGGATATCCGATGGCGGAAGGTTCTAGAA
  CCAATGCCAAGCACTGCTGAGATTAGCACCTCTGGGGCTGTTCTTAAGATCTTCAATATTCAGCTAGAAG
  ATGAAGGCATCTATGAATGTGAGGCTGAGAACATTAGAGGAAAGGATAAACATCAAGCAAGAATTTATGT
  TCAAGCATTCCCTGAGTGGGTAGAACACATCAATGACACAGAGGTGGACATAGGCAGTGATCTCTACTGG
  CCTTGTGTGGCCACAGGAAAGCCCATCCCTACAATCCGATGGTTGAAAAATGGATATGCGTATCATAAAG
  GGGAATTAAGACTGTATGATGTGACTTTTGAAAATGCCGGAATGTATCAGTGCATAGCTGAAAACACATA
  TGGAGCCATTTATGCAAATGCTGAGTTGAAGATCTTGGCGTTGGCTCCAACTTTTGAAATGAATCCTATG
  AAGAAAAAGATCCTGGCTGCTAAAGGTGGAAGGGTGATAATTGAATGCAAACCTAAAGCTGCACCGAAAC
  CAAAGTTTTCATGGAGTAAAGGGACAGAGTGGCTTGTCAATAGCAGCAGAATACTCATTTGGGAAGATGG
  TAGCTTGGAAATCAACAACATTACAAGGAATGATGGAGGTATCTATACATGCTTTGCAGAAAATAACAGA
  GGGAAAGCTAATAGCACTGGAACCCTTGTTATCACAGATCCTACGCGAATTATATTGGCCCCAATTAATG
  CCGATATCACAGTTGGAGAAAACGCCACCATGCAGTGTGCTGCGTCCTTTGATCCTGCCTTGGATCTCAC
  ATTTGTTTGGTCCTTCAATGGCTATGTGATCGATTTTAACAAAGAGAATATTCACTACCAGAGGAATTTT
  ATGCTGGATTCCAATGGGGAATTACTAATCCGAAATGCGCAGCTGAAACATGCTGGAAGATACACATGCA
  CTGCCCAGACAATTGTGGACAATTCTTCAGCTTCAGCTGACCTTGTAGTGAGAGGTAAAAACAGAAAAGG
  TGGAGAGAAAAACATGGTGGATTCCTTTCTCCCCGTCTGTGCAAGCCTGCCTCCAACCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233027 representing NM_001256064
  Red=Cloning site Green=Tags(s)
  
  MKMWLLVSHLVIISITTCLAEFTWYRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARAS
  PFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPP
  EERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYS
  CFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLE
  PMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYW
  PCVATGKPIPTIRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTYGAIYANAELKILALAPTFEMNPM
  KKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNR
  GKANSTGTLVITDPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKENIHYQRNF
  MLDSNGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGKNRKGGEKNMVDSFLPVCASLPPTW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256064
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256064.1, NP_001242993.1
• RefSeq Size: 3528 bp
• RefSeq ORF: 1884 bp
• Locus ID: 1272
• Cytogenetics: 12q12
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 71.1 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]'