Growth hormone receptor (GHR) (NM_001242405) Human Tagged ORF Clone

CAT#: RC233040

GHR (Myc-DDK tagged) - Homo sapiens growth hormone receptor (GHR), transcript variant 8

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GHR
• Synonyms: GHBP; GHIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233040 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGGATCTCTGGCAGCTGCTGTTGACCTTGGCACTGGCAGGATCAAGTGATGCTTTTTCTGGAAGTGA
  GGCCACAGCAGCTATCCTTAGCAGAGCACCCTGGAGTCTGCAAAGTGTTAATCCAGGCCTAAAGACAAAT
  TCTTCTAAGGAGCCTAAATTCACCAAGTGCCGTTCACCTGAGCGAGAGACTTTTTCATGCCACTGGACAG
  ATGAGGTTCATCATGGTACAAAGAACCTAGGACCCATACAGCTGTTCTATACCAGAAGGAACACTCAAGA
  ATGGACTCAAGAATGGAAAGAATGCCCTGATTATGTTTCTGCTGGGGAAAACAGCTGTTACTTTAATTCA
  TCGTTTACCTCCATCTGGATACCTTATTGTATCAAGCTAACTAGCAATGGTGGTACAGTGGATGAAAAGT
  GTTTCTCTGTTGATGAAATAGTGCAACCAGATCCACCCATTGCCCTCAACTGGACTTTACTGAACGTCAG
  TTTAACTGGGATTCATGCAGATATCCAAGTGAGATGGGAAGCACCACGCAATGCAGATATTCAGAAAGGG
  TGGATGGTTCTGGAGTATGAACTTCAATACAAAGAAGTAAATGAAACTAAATGGAAAATGATGGACCCTA
  TATTGACAACATCAGTTCCAGTGTACTCATTGAAAGTGGATAAGGAATATGAAGTGCGTGTGAGATCCAA
  ACAACGAAACTCTGGAAATTATGGCGAGTTCAGTGAGGTGCTCTATGTAACACTTCCTCAGATGAGCCAA
  TTTACATGTGAAGAAGATTTCTACTTTCCATGGCTCTTAATTATTATCTTTGGAATATTTGGGCTAACAG
  TGATGCTATTTGTATTCTTATTTTCTAAACAGCAAAGGATTAAAATGCTGATTCTGCCCCCAGTTCCAGT
  TCCAAAGATTAAAGGAATCGATCCAGATCTCCTCAAGGAAGGAAAATTAGAGGAGGTGAACACAATCTTA
  GCCATTCATGATAGCTATAAACCCGAATTCCACAGTGATGACTCTTGGGTTGAATTTATTGAGCTAGATA
  TTGATGAGCCAGATGAAAAGACTGAGGAATCAGACACAGACAGACTTCTAAGCAGTGACCATGAGAAATC
  ACATAGTAACCTAGGGGTGAAGGATGGCGACTCTGGACGTACCAGCTGTTGTGAACCTGACATTCTGGAG
  ACTGATTTCAATGCCAATGACATACATGAGGGTACCTCAGAGGTTGCTCAGCCACAGAGGTTAAAAGGGG
  AAGCAGATCTCTTATGCCTTGACCAGAAGAATCAAAATAACTCACCTTATCATGATGCTTGCCCTGCTAC
  TCAGCAGCCCAGTGTTATCCAAGCAGAGAAAAACAAACCACAACCACTTCCTACTGAAGGAGCTGAGTCA
  ACTCACCAAGCTGCCCATATTCAGCTAAGCAATCCAAGTTCACTGTCAAACATCGACTTTTATGCCCAGG
  TGAGCGACATTACACCAGCAGGTAGCGTGGTCCTTTCCCCGGGCCAAAAGAATAAGGCAGGGATGTCCCA
  ATGTGACATGCACCCGGAAATGGTCTCACTCTGCCAAGAAAACTTCCTTATGGACAATGCCTACTTCTGT
  GAGGCAGATGCCAAAAAGTGCATCCCTGTGGCTCCTCACATCAAGGTTGAATCACACATACAGCCAAGCT
  TAAACCAAGAGGACATTTACATCACCACAGAAAGCCTTACCACTGCTGCTGGGAGGCCTGGGACAGGAGA
  ACATGTTCCAGGTTCTGAGATGCCTGTCCCAGACTATACCTCCATTCATATAGTACAGTCCCCACAGGGC
  CTCATACTCAATGCGACTGCCTTGCCCTTGCCTGACAAAGAGTTTCTCTCATCATGTGGCTATGTGAGCA
  CAGACCAACTGAACAAAATCATGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233040 protein sequence
  Red=Cloning site Green=Tags(s)
  
  XWISGSCC*PWHWQDQVMLFLEVRPQQLSLAEHPGVCKVLIQA*RQILLRSLNSPSAVHLSERLFHATGQ
  MRFIMVQRT*DPYSCSIPEGTLKNGLKNGKNALIMFLLGKTAVTLIHRLPPSGYLIVSS*LAMVVQWMKS
  VSLLMK*CNQIHPLPSTGLY*TSV*LGFMQISK*DGKHHAMQIFRKGGWFWSMNFNTKK*MKLNGK*WTL
  Y*QHQFQCTH*KWIRNMKCV*DPNNETLEIMASSVRCSM*HFLR*ANLHVKKISTFHGS*LLSLEYLG*Q
  *CYLYSYFLNSKGLKC*FCPQFQFQRLKESIQISSRKEN*RR*TQS*PFMIAINPNSTVMTLGLNLLS*I
  LMSQMKRLRNQTQTDF*AVTMRNHIVT*G*RMATLDVPAVVNLTFWRLISMPMTYMRVPQRLLSHRG*KG
  KQISYALTRRIKITHLIMMLALLLSSPVLSKQRKTNHNHFLLKELSQLTKLPIFS*AIQVHCQTSTFMPR
  *ATLHQQVAWSFPRAKRIRQGCPNVTCTRKWSHSAKKTSLWTMPTSVRQMPKSASLWLLTSRLNHTYSQA
  *TKRTFTSPQKALPLLLGGLGQENMFQVLRCLSQTIPPFI*YSPHRASYSMRLPCPCLTKSFSHHVAM*A
  QTN*TKSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242405
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242405.1, NM_001242405.2, NP_001229334.1
• RefSeq Size: 4419 bp
• RefSeq ORF: 1917 bp
• Locus ID: 2690
• Cytogenetics: 5p13.1-p12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction
• MW: 71.5 kDa
• Gene Summary: 'This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]'