CUX1 (NM_001202546) Human Tagged ORF Clone

CAT#: RC233045

CUX1 (Myc-DDK tagged) - Homo sapiens cut-like homeobox 1 (CUX1), transcript variant 7

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CUX1
• Synonyms: CASP; CDP; CDP/Cut; CDP1; Clox; COY1; CUTL1; CUX; Cux/CDP; GDDI; GOLIM6; Nbla10317; p75; p100; p110; p200
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233045 representing NM_001202546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCAATGTGGGATCGATGTTTCAATATTGGAAGCGCTTTGATTTACAGCAGCTGCAGGATTTGC
  GCAAGCAGGTAGCGCCGCTGCTGAAGAGTTTCCAAGGAGAGATTGATGCACTGAGTAAAAGAAGCAAGGA
  AGCTGAAGCAGCTTTCTTGAATGTCTACAAAAGATTGATTGACGTCCCAGATCCCGTACCAGCTTTGGAT
  CTCGGACAGCAACTCCAGCTCAAAGTGCAGCGCCTGCACGATATTGAAACAGAGAACCAGAAACTTAGGG
  AAACTCTGGAAGAATACAACAAGGAATTTGCTGAAGTGAAAAATCAAGAGGTTACGATAAAAGCACTTAA
  AGAGAAAATCCGAGAATATGAACAGACACTGAAGAACCAAGCCGAAACCATAGCTCTTGAGAAGGAACAG
  AAGTTACAGAATGACTTTGCAGAAAAGGAGAGAAAGCTGCAGGAGACACAGATGTCCACCACCTCAAAGC
  TGGAGGAAGCTGAGCATAAGGTTCAGAGCCTACAAACAGCCCTGGAAAAAACTCGAACAGAATTATTTGA
  CCTGAAAACCAAATACGATGAAGAAACTACTGCAAAGGCCGACGAGATTGAAATGATCATGACGGACCTT
  GAAAGGGCAAACCAGAGGGCAGAGGTGGCTCAGAGAGAGGCGGAGACCTTAAGGGAACAGCTCTCATCGG
  CCAATCACTCCCTCCAGCTGGCCTCACAGATCCAGAAGGCACCAGACGTGGCCATAGAGGTGCTGACCCG
  CTCCAGCCTAGAAGTTGAGTTGGCCGCCAAGGAGCGGGAGATCGCACAGCTGGTGGAGGACGTGCAGAGA
  CTCCAGGCCAGCCTCACCAAGCTGCGGGAGAATTCGGCCAGCCAGATCTCACAGCTTGAGCAGCAGCTGA
  GCGCCAAAAACAGCACACTCAAACAACTGGAAGAAAAACTCAAAGGCCAGGCTGACTATGAAGAGGTGAA
  GAAAGAGCTGAACATTCTGAAGTCCATGGAGTTTGCACCGTCCGAGGGCGCTGGGACACAGGATGCGGCC
  AAGCCCCTGGAGGTGCTGTTGCTGGAGAAGAACCGCTCGCTGCAGTCCGAGAACGCCGCGCTGCGCATCT
  CCAACAGCGACCTGAGCGGACGCTGTGCGGAGCTGCAAGTCCGTATCACTGAGGCTGTGGCCACAGCCAC
  TGAGCAGAGAGAGCTGATCGCCCGCCTGGAGCAGGACCTGAGCATCATTCAGTCCATCCAGCGGCCCGAT
  GCCGAGGGTGCCGCTGAGCACCGCCTGGAGAAGATCCCAGAGCCCATCAAAGAGGCCACTGCCCTATTCT
  ACGGACCTGCAGCACCAGCCAGCGGTGCCCTCCCAGAGGGCCAGGTGGATTCACTGCTTTCCATCATCTC
  CAGCCAGAGGGAGCGCTTCCGTGCCCGGAACCAGGAGCTTGAGGCCGAGAACCGCCTGGCCCAGCACACC
  CTCCAGGCCCTGCAGAGTGAGCTGGACAGCCTGCGCGCCGACAACATCAAGCTCTTTGAGAAGATCAAGT
  TCCTGCAGAGCTACCCTGGCCGGGGCAGCGGCAGTGATGACACGGAGCTGCGGTACTCGTCCCAGTACGA
  GGAGCGCCTGGACCCCTTCTCCTCCTTCAGCAAGCGGGAGCGGCAGAGGAAGTACCTGAGCTTGAGTCCC
  TGGGACAAGGCCACCCTCAGCATGGGGCGTCTGGTTCTCTCCAACAAGATGGCGCGCACCATCGGCTTCT
  TCTACACACTGTTCCTGCACTGCCTGGTCTTCCTGGTGCTCTACAAGCTGGCATGGAGCGAGAGCATGGA
  GAGGGACTGTGCCACCTTCTGCGCCAAGAAGTTCGCTGACCACCTGCACAAGTTCCACGAGAATGACAAC
  GGGGCTGCGGCTGGTGACTTGTGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233045 representing NM_001202546
  Red=Cloning site Green=Tags(s)
  
  MAANVGSMFQYWKRFDLQQLQDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALD
  LGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQ
  KLQNDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDL
  ERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVAIEVLTRSSLEVELAAKEREIAQLVEDVQR
  LQASLTKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPSEGAGTQDAA
  KPLEVLLLEKNRSLQSENAALRISNSDLSGRCAELQVRITEAVATATEQRELIARLEQDLSIIQSIQRPD
  AEGAAEHRLEKIPEPIKEATALFYGPAAPASGALPEGQVDSLLSIISSQRERFRARNQELEAENRLAQHT
  LQALQSELDSLRADNIKLFEKIKFLQSYPGRGSGSDDTELRYSSQYEERLDPFSSFSKRERQRKYLSLSP
  WDKATLSMGRLVLSNKMARTIGFFYTLFLHCLVFLVLYKLAWSESMERDCATFCAKKFADHLHKFHENDN
  GAAAGDLWQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001202546
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202546.1, NM_001202546.2, NP_001189475.1
• RefSeq Size: 2818 bp
• RefSeq ORF: 1920 bp
• Locus ID: 1523
• Cytogenetics: 7q22.1
• Protein Families: Transcription Factors, Transmembrane
• MW: 73.3 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]'