TNK1 (NM_001251902) Human Tagged ORF Clone

CAT#: RC233066

TNK1 (Myc-DDK tagged) - Homo sapiens tyrosine kinase, non-receptor, 1 (TNK1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TNK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233066 representing NM_001251902
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCCTGAGGCTGGCTCCCTGTGGCTACTGAAGCTGCTCCGGGACATCCAGTTGGCCCAGTTTTACT
  GGCCCATCCTTGAGGAGCTTAATGTCACTCGGCCAGAGCACTTCGACTTTGTAAAGCCTGAGGACCTGGA
  CGGCATTGGCATGGGCCGGCCTGCCCAGCGCAGACTGTCCGAAGCTCTGAAAAGGCTACGTTCTGGGCCT
  AAGTCTAAGAACTGGGTCTACAAGATCCTTGGAGGTTTTGCCCCTGAGCACAAGGAGCCCACCCTGCCCT
  CGGACAGCCCACGGCACCTCCCTGAGCCAGAGGGGGGCCTCAAGTGTCTGATCCCAGAGGGTGCTGTTTG
  CAGAGGGGAGCTGCTGGGTTCAGGCTGCTTCGGTGTGGTGCACCGAGGGCTGTGGACGCTGCCCAGTGGC
  AAGAGTGTCCCAGTGGCTGTCAAGTCCCTCCGGGTAGGTCCCGAAGGCCCGATGGGCACAGAACTGGGGG
  ACTTCCTGCGAGAGGTATCGGTCATGATGAACTTGGAGCACCCACACGTGCTGCGTCTGCACGGCCTTGT
  ACTGGGCCAGCCTCTGCAGATGGTGATGGAGCTGGCGCCACTGGGCTCCCTGCACGCGCGCCTAACGGCC
  CCGGCCCCGACACCCCCGCTGCTCGTGGCCCTGCTCTGCCTCTTCCTGCGGCAGCTGGCGGGAGCCATGG
  CGTACCTGGGGGCCCGCGGGCTGGTGCACCGAGACCTCGCTACGCGCAACCTACTGCTGGCGTCGCCGCG
  CACCATCAAGGTGGCTGACTTCGGGCTGGTGCGGCCTCTGGGCGGTGCCCGGGGCCGCTACGTCATGGGC
  GGGCCCCGCCCTATCCCCTACGCCTGGTGTGCCCCAGAGAGCCTGCGCCACGGAGCCTTCTCGTCTGCCT
  CGGACGTGTGGATGTTTGGGGTGACGCTGTGGGAGATGTTCTCCGGGGGCGAGGAACCCTGGGCCGGGGT
  CCCACCGTACCTCATCCTGCAGCGGCTGGAGGACAGAGCCCGGCTGCCTAGGCCTCCCCTCTGCTCCAGG
  GCCCTCTACTCCCTCGCCTTGCGCTGCTGGGCCCCCCACCCTGCCGACCGGCCTAGCTTTTCCCACCTGG
  AGGGGCTGCTGCAAGAGGCCGGGCCTTCGGAAGCATGTTGTGTGAGGGATGTCACAGAACCAGGCGCCCT
  GAGGATGGAGACTGGTGACCCCATCACAGTCATCGAGGGCAGCTCCTCTTTCCACAGCCCCGACTCCACA
  ATCTGGAAGGGCCAGAATGGTCGCACCTTCAAAGTGGGCAGCTTCCCAGCCTCGGCAGTGACGCTGGCAG
  ATGCGGGGGGCTTGCCAGCCACCCGTCCAGTCCACAGAGGCACCCCTGCCCGGGGAGATCAACACCCAGG
  AAGCATAGATGGAGACAGAAAGAAGGCAAATCTTTGGGATGCGCCCCCAGCACGGGGCCAGAGGAGGAAC
  ATGCCCCTGGAGAGGATGAAAGGCATTTCCAGGAGTCTGGAGTCAGTTCTGTCCCTCGGTCCTCGTCCCA
  CAGGGGGTGGTTCAAGCCCCCCTGAAATTCGACAAGCCAGAGCTGTGCCCCAGGGACCTCCAGGCCTGCC
  TCCACGCCCACCTTTATCCTCTAGCTCTCCTCAGCCCAGCCAGCCCTCTAGGGAGAGGCTTCCCTGGCCC
  AAAAGAAAACCCCCACACAATCACCCCATGGGAATGCCTGGAGCCCGTAAAGCCGCTGCCCTCTCTGGAG
  GCCTCTTGTCCGATCCTGAGTTGCAGAGGAAGATTATGGAGGTGGAGCTGAGTGTGCATGGGGTCACCCA
  CCAGGAGTGCCAGACAGCACTAGGAGCCACTGGGGGAGATGTGGTTTCTGCCATCCGGAACCTCAAGGTA
  GATCAGCTCTTCCACCTGAGTAGCCGGTCCAGAGCTGACTGCTGGCGCATCCTGGAGCATTACCAGTGGG
  ACCTCTCAGCTGCCAGCCGCTATGTCCTGGCCAGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233066 representing NM_001251902
  Red=Cloning site Green=Tags(s)
  
  MLPEAGSLWLLKLLRDIQLAQFYWPILEELNVTRPEHFDFVKPEDLDGIGMGRPAQRRLSEALKRLRSGP
  KSKNWVYKILGGFAPEHKEPTLPSDSPRHLPEPEGGLKCLIPEGAVCRGELLGSGCFGVVHRGLWTLPSG
  KSVPVAVKSLRVGPEGPMGTELGDFLREVSVMMNLEHPHVLRLHGLVLGQPLQMVMELAPLGSLHARLTA
  PAPTPPLLVALLCLFLRQLAGAMAYLGARGLVHRDLATRNLLLASPRTIKVADFGLVRPLGGARGRYVMG
  GPRPIPYAWCAPESLRHGAFSSASDVWMFGVTLWEMFSGGEEPWAGVPPYLILQRLEDRARLPRPPLCSR
  ALYSLALRCWAPHPADRPSFSHLEGLLQEAGPSEACCVRDVTEPGALRMETGDPITVIEGSSSFHSPDST
  IWKGQNGRTFKVGSFPASAVTLADAGGLPATRPVHRGTPARGDQHPGSIDGDRKKANLWDAPPARGQRRN
  MPLERMKGISRSLESVLSLGPRPTGGGSSPPEIRQARAVPQGPPGLPPRPPLSSSSPQPSQPSRERLPWP
  KRKPPHNHPMGMPGARKAAALSGGLLSDPELQRKIMEVELSVHGVTHQECQTALGATGGDVVSAIRNLKV
  DQLFHLSSRSRADCWRILEHYQWDLSAASRYVLARP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001251902
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001251902.1, NP_001238831.1
• RefSeq Size: 2927
• RefSeq ORF: 2001
• Locus ID: 8711
• Protein Families: Druggable Genome, Protein Kinase
• MW: 72.9 kDa
• Gene Summary: The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways, mediating cellular proliferation, survival, and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues, thus may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]