ADGRE1 (NM_001256255) Human Tagged ORF Clone

CAT#: RC233097

EMR1 (Myc-DDK tagged) - Homo sapiens egf-like module containing, mucin-like, hormone receptor-like 1 (EMR1), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADGRE1
• Synonyms: EMR1; TM7LN3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233097 representing NM_001256255
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGGCTTCAACCTGCTCCTCTTCTGGGGATGTTGTGTTATGCACAGCTGGGAAGGGCACATAAGAC
  CCACACGGAAACCAAACACAAAGGGTAATAACTGTAGAGACAGTACCTTGTGCCCAGCTTATGCCACCTG
  CACCAATACAGTGGACAGTTACTATTGCGCTTGCAAACAAGGCTTCCTGTCCAGCAATGGGCAAAATCAC
  TTCAAGGATCCAGGAGTGCGATGCAAAGATATTGATGAATGTTCTCAAAGCCCCCAGCCCTGTGGTCCTA
  ACTCATCCTGCAAAAACCTGTCAGGGAGGTACAAGTGCAGCTGTTTAGATGGTTTCTCTTCTCCCACTGG
  AAATGACTGGGTCCCAGGAAAGCCGGGCAATTTCTCCTGTACTGATATCAATGAGTGCCTCACCAGCAGG
  GTTCTCTTCAAATGTAAGGAAGATGTGATACCCGATAATAAGCAGATCCAGCAATGCCAAGAGGGAACCG
  CAGTGAAACCTGCATATGTCTCCTTTTGTGCACAAATAAATAACATCTTCAGCGTTCTGGACAAAGTGTG
  TGAAAATAAAACGACCGTAGTTTCTCTGAAGAATACAACTGAGAGCTTTGTCCCTGTGCTTAAACAAATA
  TCCACGTGGACTAAATTCACCAAGGAAGAGACGTCCTCCCTGGCCACAGTCTTCCTGGAGAGTGTGGAAA
  GCATGACACTGGCATCTTTTTGGAAACCCTCAGCAAATATCACTCCGGCTGTTCGGACGGAATACTTAGA
  CATTGAGAGCAAAGTTATCAACAAAGAATGCAGTGAAGAGAATGTGACGTTGGACTTGGTAGCCAAGGGG
  GATAAGATGAAGATCGGGTGTTCCACAATTGAGGAATCTGAATCCACAGAGACCACTGGTGTGGCTTTTG
  TCTCCTTTGTGGGCATGGAATCGGTTTTAAATGAGCGCTTCTTCAAAGACCACCAGGCTCCCTTGACCAC
  CTCTGAGATCAAGCTGAAGATGAATTCTCGAGTCGTTGGGGGCATAATGACTGGAGAGAAGAAAGACGGC
  TTCTCAGATCCAATCATCTACACTCTGGAGAACATTCAGCCAAAGCAGAAGTTTGAGAGGCCCATCTGTG
  TTTCCTGGAGCACTGATGTGAAGGGTGGAAGATGGACATCCTTTGGCTGTGTGATCCTGGAAGCTTCTGA
  GACATATACCATCTGCAGCTGTAATCAGATGGCAAATCTTGCCGTTATCATGGCGTCTGGGGAGCTCACG
  ATGGACTTTTCCTTGTACATCATTAGCCATGTAGGCATTATCATCTCCTTGGTGTGCCTCGTCTTGGCCA
  TCGCCACCTTTCTGCTGTGTCGCTCCATCCGAAATCACAACACCTACCTCCACCTGCACCTCTGCGTGTG
  TCTCCTCTTGGCGAAGACTCTCTTCCTCGCCGGTATACACAAGACTGACAACAAGATGGGCTGCGCCATC
  ATCGCGGGCTTCCTGCACTACCTTTTCCTTGCCTGCTTCTTCTGGATGCTGGTGGAGGCTGTGATACTGT
  TCTTGATGGTCAGAAACCTGAAGGTGGTGAATTACTTCAGCTCTCGCAACATCAAGATGCTGCACATCTG
  TGCCTTTGGTTATGGGCTGCCGATGCTGGTGGTGGTGATCTCTGCCAGTGTGCAGCCACAGGGCTATGGA
  ATGCATAATCGCTGCTGGCTGAATACAGAGACAGGGTTCATCTGGAGTTTCTTGGGGCCAGTTTGCACAG
  TTATAGTGATCAACTCCCTTCTCCTGACCTGGACCTTGTGGATCCTGAGGCAGAGGCTTTCCAGTGTTAA
  TGCCGAAGTCTCAACGCTAAAAGACACCAGGTTACTGACCTTCAAGGCCTTTGCCCAGCTCTTCATCCTG
  GGCTGCTCCTGGGTGCTGGGCATTTTTCAGATTGGACCTGTGGCAGGTGTCATGGCTTACCTGTTCACCA
  TCATCAACAGCCTGCAGGGGGCCTTCATCTTCCTCATCCACTGTCTGCTCAACGGCCAGGTACGAGAAGA
  ATACAAGAGGTGGATCACTGGGAAGACGAAGCCCAGCTCCCAGTCCCAGACCTCAAGGATCTTGCTGTCC
  TCCATGCCATCCGCTTCCAAGACGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233097 representing NM_001256255
  Red=Cloning site Green=Tags(s)
  
  MRGFNLLLFWGCCVMHSWEGHIRPTRKPNTKGNNCRDSTLCPAYATCTNTVDSYYCACKQGFLSSNGQNH
  FKDPGVRCKDIDECSQSPQPCGPNSSCKNLSGRYKCSCLDGFSSPTGNDWVPGKPGNFSCTDINECLTSR
  VLFKCKEDVIPDNKQIQQCQEGTAVKPAYVSFCAQINNIFSVLDKVCENKTTVVSLKNTTESFVPVLKQI
  STWTKFTKEETSSLATVFLESVESMTLASFWKPSANITPAVRTEYLDIESKVINKECSEENVTLDLVAKG
  DKMKIGCSTIEESESTETTGVAFVSFVGMESVLNERFFKDHQAPLTTSEIKLKMNSRVVGGIMTGEKKDG
  FSDPIIYTLENIQPKQKFERPICVSWSTDVKGGRWTSFGCVILEASETYTICSCNQMANLAVIMASGELT
  MDFSLYIISHVGIIISLVCLVLAIATFLLCRSIRNHNTYLHLHLCVCLLLAKTLFLAGIHKTDNKMGCAI
  IAGFLHYLFLACFFWMLVEAVILFLMVRNLKVVNYFSSRNIKMLHICAFGYGLPMLVVVISASVQPQGYG
  MHNRCWLNTETGFIWSFLGPVCTVIVINSLLLTWTLWILRQRLSSVNAEVSTLKDTRLLTFKAFAQLFIL
  GCSWVLGIFQIGPVAGVMAYLFTIINSLQGAFIFLIHCLLNGQVREEYKRWITGKTKPSSQSQTSRILLS
  SMPSASKTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256255
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256255.1, NP_001243184.1
• RefSeq Size: 2630 bp
• RefSeq ORF: 2130 bp
• Locus ID: 2015
• Cytogenetics: 19p13.3-p13.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 79.4 kDa
• Gene Summary: 'This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'