ADGRE1 (NM_001256254) Human Tagged ORF Clone

CAT#: RC233116

EMR1 (Myc-DDK tagged) - Homo sapiens egf-like module containing, mucin-like, hormone receptor-like 1 (EMR1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADGRE1
• Synonyms: EMR1; TM7LN3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233116 representing NM_001256254
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGGCTTCAACCTGCTCCTCTTCTGGGGATGTTGTGTTATGCACAGCTGGGAAGGGCACATAAGAC
  CCACACGGAAACCAAACACAAAGGGTAATAACTGTAGAGACAGTACCTTGTGCCCAGCTTATGCCACCTG
  CACCAATACAGTGGACAGTTACTATTGCGCTTGCAAACAAGGCTTCCTGTCCAGCAATGGGCAAAATCAC
  TTCAAGGATCCAGGAGTGCGATGCAAAGATATTGATGAATGCACTGAAATGTGCCCCATCAATTCAACAT
  GCACCAACACTCCTGGGAGCTACTTTTGCACCTGCCACCCTGGCTTTGCACCAAGCAATGGACAGTTGAA
  TTTCACAGACCAAGGAGTGGAATGTAGAGATATTGATGAGTGCCGCCAAGATCCATCAACCTGTGGTCCT
  AATTCTATCTGCACCAATGCCCTGGGCTCCTACAGCTGTGGCTGCATTGCAGGCTTTCATCCCAATCCAG
  AAGGCTCCCAGAAAGATGGCAACTTCAGCTGCCAAAGGGTTCTCTTCAAATGTAAGGAAGATGTGATACC
  CGATAATAAGCAGATCCAGCAATGCCAAGAGGGAACCGCAGTGAAACCTGCATATGTCTCCTTTTGTGCA
  CAAATAAATAACATCTTCAGCGTTCTGGACAAAGTGTGTGAAAATAAAACGACCGTAGTTTCTCTGAAGA
  ATACAACTGAGAGCTTTGTCCCTGTGCTTAAACAAATATCCACGTGGACTAAATTCACCAAGGAAGAGAC
  GTCCTCCCTGGCCACAGTCTTCCTGGAGAGTGTGGAAAGCATGACACTGGCATCTTTTTGGAAACCCTCA
  GCAAATATCACTCCGGCTGTTCGGACGGAATACTTAGACATTGAGAGCAAAGTTATCAACAAAGAATGCA
  GTGAAGAGAATGTGACGTTGGACTTGGTAGCCAAGGGGGATAAGATGAAGATCGGGTGTTCCACAATTGA
  GGAATCTGAATCCACAGAGACCACTGGTGTGGCTTTTGTCTCCTTTGTGGGCATGGAATCGGTTTTAAAT
  GAGCGCTTCTTCAAAGACCACCAGGCTCCCTTGACCACCTCTGAGATCAAGCTGAAGATGAATTCTCGAG
  TCGTTGGGGGCATAATGACTGGAGAGAAGAAAGACGGCTTCTCAGATCCAATCATCTACACTCTGGAGAA
  CATTCAGCCAAAGCAGAAGTTTGAGAGGCCCATCTGTGTTTCCTGGAGCACTGATGTGAAGGGTGGAAGA
  TGGACATCCTTTGGCTGTGTGATCCTGGAAGCTTCTGAGACATATACCATCTGCAGCTGTAATCAGATGG
  CAAATCTTGCCGTTATCATGGCGTCTGGGGAGCTCACGATGGACTTTTCCTTGTACATCATTAGCCATGT
  AGGCATTATCATCTCCTTGGTGTGCCTCGTCTTGGCCATCGCCACCTTTCTGCTGTGTCGCTCCATCCGA
  AATCACAACACCTACCTCCACCTGCACCTCTGCGTGTGTCTCCTCTTGGCGAAGACTCTCTTCCTCGCCG
  GTATACACAAGACTGACAACAAGATGGGCTGCGCCATCATCGCGGGCTTCCTGCACTACCTTTTCCTTGC
  CTGCTTCTTCTGGATGCTGGTGGAGGCTGTGATACTGTTCTTGATGGTCAGAAACCTGAAGGTGGTGAAT
  TACTTCAGCTCTCGCAACATCAAGATGCTGCACATCTGTGCCTTTGGTTATGGGCTGCCGATGCTGGTGG
  TGGTGATCTCTGCCAGTGTGCAGCCACAGGGCTATGGAATGCATAATCGCTGCTGGCTGAATACAGAGAC
  AGGGTTCATCTGGAGTTTCTTGGGGCCAGTTTGCACAGTTATAGTGATCAACTCCCTTCTCCTGACCTGG
  ACCTTGTGGATCCTGAGGCAGAGGCTTTCCAGTGTTAATGCCGAAGTCTCAACGCTAAAAGACACCAGGT
  TACTGACCTTCAAGGCCTTTGCCCAGCTCTTCATCCTGGGCTGCTCCTGGGTGCTGGGCATTTTTCAGAT
  TGGACCTGTGGCAGGTGTCATGGCTTACCTGTTCACCATCATCAACAGCCTGCAGGGGGCCTTCATCTTC
  CTCATCCACTGTCTGCTCAACGGCCAGGTACGAGAAGAATACAAGAGGTGGATCACTGGGAAGACGAAGC
  CCAGCTCCCAGTCCCAGACCTCAAGGATCTTGCTGTCCTCCATGCCATCCGCTTCCAAGACGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233116 representing NM_001256254
  Red=Cloning site Green=Tags(s)
  
  MRGFNLLLFWGCCVMHSWEGHIRPTRKPNTKGNNCRDSTLCPAYATCTNTVDSYYCACKQGFLSSNGQNH
  FKDPGVRCKDIDECTEMCPINSTCTNTPGSYFCTCHPGFAPSNGQLNFTDQGVECRDIDECRQDPSTCGP
  NSICTNALGSYSCGCIAGFHPNPEGSQKDGNFSCQRVLFKCKEDVIPDNKQIQQCQEGTAVKPAYVSFCA
  QINNIFSVLDKVCENKTTVVSLKNTTESFVPVLKQISTWTKFTKEETSSLATVFLESVESMTLASFWKPS
  ANITPAVRTEYLDIESKVINKECSEENVTLDLVAKGDKMKIGCSTIEESESTETTGVAFVSFVGMESVLN
  ERFFKDHQAPLTTSEIKLKMNSRVVGGIMTGEKKDGFSDPIIYTLENIQPKQKFERPICVSWSTDVKGGR
  WTSFGCVILEASETYTICSCNQMANLAVIMASGELTMDFSLYIISHVGIIISLVCLVLAIATFLLCRSIR
  NHNTYLHLHLCVCLLLAKTLFLAGIHKTDNKMGCAIIAGFLHYLFLACFFWMLVEAVILFLMVRNLKVVN
  YFSSRNIKMLHICAFGYGLPMLVVVISASVQPQGYGMHNRCWLNTETGFIWSFLGPVCTVIVINSLLLTW
  TLWILRQRLSSVNAEVSTLKDTRLLTFKAFAQLFILGCSWVLGIFQIGPVAGVMAYLFTIINSLQGAFIF
  LIHCLLNGQVREEYKRWITGKTKPSSQSQTSRILLSSMPSASKTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256254
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256254.1, NP_001243183.1
• RefSeq Size: 2738 bp
• RefSeq ORF: 2238 bp
• Locus ID: 2015
• Cytogenetics: 19p13.3-p13.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 83.2 kDa
• Gene Summary: 'This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'