GLT28D1 (ALG13) (NM_001257237) Human Tagged ORF Clone

CAT#: RC233188

ALG13 (Myc-DDK tagged) - Homo sapiens ALG13, UDP-N-acetylglucosaminyltransferase subunit (ALG13), transcript variant 10

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALG13
• Synonyms: CDG1S; CXorf45; EIEE36; GLT28D1; MDS031; TDRD13; YGL047W
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233188 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAATCATCAGCTGGAACTGGCAAAGCAGCTACACAAAGAGGGTCATCTCTTCTATTGTACCTGCA
  GGGTCCTGACTTGTCCTGGGCAAGCCAAGTCCATTGCTTCTGCTCCTGGGAAGTGCCAAGATTCTGCAGC
  GCTGACTTCAACTGCCTTTTCAGGCCTAGACTTTGGGCTGCTTTCCGGATACCTGCATAAGCAAGCCCTT
  GTTACTGCTACCCATCCTACCTGCACCCTGCTTTTTCCCTCTTGCCACGCTTTTTTTCCTCTCCCTCTTA
  CCCCCACCCTGTACAAAATGCATAAAGGATGGAAAAACTACTGCAGCCAGAAGTCTTTGAATGAGGCATC
  AATGGATGAATATTTAGGCAGCTTAGGGCTGTTTCGAAAGCTGACTGCCAAGGATGCCTCTTGCCTCTTT
  CGGGCCATTTCGGAGCAGTTGTTTTGCAGCCAGGTCCATCATTTGGAAATCAGGAAGGCTTGTGTCTCAT
  ATATGAGGGAAAATCAACAAACTTTTGAGTCTTATGTGGAGGGATCTTTTGAGAAATACCTGGAACGGTT
  GGGAGATCCCAAGGAAAGTGCTGGCCAGCTGGAAATAAGAGCTCTTTCTCTAATTTATAATCGGGATTTC
  ATTCTTTATCGCTTTCCTGGAAAACCTCCAACTTATGTCACAGATAATGGCTATGAAGACAAGATTCTAC
  TCTGCTACTCAAGTAGTGGTCACTATGATTCTGTGTACTCAAAACAATTTCAGTCAAGTGCAGCTGTTTG
  TCAGGCTGTATTGTACGAAATTCTCTATAAAGATGTGTTTGTTGTGGATGAAGAAGAGTTGAAGACTGCG
  ATTAAATTGTTTCGAAGTGGTTCTAAGAAGAACAGAAATAATGCTGTAACTGGAAGCGAGGATGCCCATA
  CTGATTACAAGAGTTCAAATCAGAATAGGATGGAAGAGTGGGGTGCCTGCTACAATGCTGAAAATATACC
  AGAGGGCTACAATAAAGGAACAGAAGAAACAAAGTCTCCAGAAAATCCATCAAAGATGCCCTTCCCCTAT
  AAGGTGCTCAAAGCCCTGGATCCAGAAATCTATCGTAATGTAGAATTTGATGTTTGGTTGGACAGCAGAA
  AAGAACTTCAAAAATCTGATTACATGGAGTATGCTGGGAGACAGTACTATTTGGGAGACAAGTGTCAGGT
  TTGCTTGGAATCAGAAGGAAGATATTATAATGCTCATATCCAGGAAGTTGGAAATGAGAACAATTCAGTA
  ACAGTCTTCATTGAAGAATTGGCGGAAAAGCATGTTGTTCCACTGGCTAACTTAAAACCAGTTACCCAAG
  TGATGTCTGTTCCTGCCTGGAATGCTATGCCCAGTCGGAAAGGAAGAGGTTACCAGAAAATGCCTGGGGG
  TTATGTCCCGGAAATAGTTATATCAGAGATGGACATAAAGCAACAGAAGAAGATGTTCAAGAAAATTCGA
  GGGAAAGAAGTTTACATGACTATGGCTTACGGCAAGGGAGACCCCCTCCTCCCACCCAGGCTGCAGCACA
  GTATGCATTATGGGCACGATCCTCCAATGCACTACTCACAGACAGCTGGCAATGTTATGTCTAATGAACA
  TTTTCATCCTCAGCATCCATCTCCGAGACAAGGTCGGGGATATGGGATGCCCAGGAATTCATCTCGGTTT
  ATAAACAGGCACAACATGCCGGGCCCTAAAGTTGATTTTTACCCAGGCCCAGGTAAAAGGTGCTGCCAGA
  GCTATGATAACTTCTCTTATAGATCTCGTTCATTTAGACGTAGTCACCGCCAGATGAGTTGTGTGAATAA
  GGAGTCCCAGTATGGATTTACCCCAGGGAATGGACAGATGCCCAGGGGCTTGGAAGAAACTATTACTTTT
  TATGAAGTTGAAGAAGGGGATGAGACTGCTTATCCAACTTTACCTAATCATGGAGGTCCCTCTACAATGG
  TTCCTGCTACTTCAGGATACTGTGTTGGAAGGCGGGGACATAGCTCAGGCAAACAGACTTTGAATTTAGA
  GGAGGGCAATGGCCAGAGTGAAAATGGGCGATATCATGAAGAATATCTTTATCGTGCAGAGCCAGACTAT
  GAAACTTCAGGTGTTTATAGCACAACTGCATCTACAGCAAACTTGTCTCTTCAGGACAGAAAGTCATGTT
  CTATGTCTCCTCAGGACACAGTTACCTCATACAACTACCCCCAGAAGATGATGGGAAATATTGCAGCAGT
  TGCAGCTTCCTGTGCCAATAATGTTCCAGCTCCAGTCTTATCTAACGGTGCAGCGGCTAATCAAGCTATT
  AGTACCACTTCAGTTTCCTCACAGAATGCTATACAGCCTCTCTTTGTATCTCCACCTACACACGGCAGGC
  CAGATACAAAAGTTTTGCAGTACTATTTCAATCTAGGATTGCAGTGCTATTACCACAGCTACTGGCACTC
  CATGGTCTATGTGCCACAGATGCAGCAGCAGCTTCATGTAGAGAATTATCCAGTCTATACTGAGCCACCT
  CTGGTAGATCAAACCGTTCCTCAATGCTACAGTGAGGTGAGGAGAGAAGATGGCATACAGGCGGAAGCAT
  CAGCAAATGATACTTTTCCGAATGCTGATTCTTCATCTGTCCCTCATGGAGCAGTCTATTATCCAGTAAT
  GTCAGATCCCTATGGGCAGCCACCTTTGCCAGGTTTTGACTCCTGCCTTCCGGTTGTGCCAGATTATTCC
  TGTGTTCCCCCCTGGCATCCAGTTGGTACAGCATATGGTGGTTCTTCTCAAATTCATGGTGCTATAAATC
  CTGGGCCAATTGGCTGTATTGCTCCATCTCCCCCAGCTTCTCATTATGTACCTCAGGGTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233188 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNNHQLELAKQLHKEGHLFYCTCRVLTCPGQAKSIASAPGKCQDSAALTSTAFSGLDFGLLSGYLHKQAL
  VTATHPTCTLLFPSCHAFFPLPLTPTLYKMHKGWKNYCSQKSLNEASMDEYLGSLGLFRKLTAKDASCLF
  RAISEQLFCSQVHHLEIRKACVSYMRENQQTFESYVEGSFEKYLERLGDPKESAGQLEIRALSLIYNRDF
  ILYRFPGKPPTYVTDNGYEDKILLCYSSSGHYDSVYSKQFQSSAAVCQAVLYEILYKDVFVVDEEELKTA
  IKLFRSGSKKNRNNAVTGSEDAHTDYKSSNQNRMEEWGACYNAENIPEGYNKGTEETKSPENPSKMPFPY
  KVLKALDPEIYRNVEFDVWLDSRKELQKSDYMEYAGRQYYLGDKCQVCLESEGRYYNAHIQEVGNENNSV
  TVFIEELAEKHVVPLANLKPVTQVMSVPAWNAMPSRKGRGYQKMPGGYVPEIVISEMDIKQQKKMFKKIR
  GKEVYMTMAYGKGDPLLPPRLQHSMHYGHDPPMHYSQTAGNVMSNEHFHPQHPSPRQGRGYGMPRNSSRF
  INRHNMPGPKVDFYPGPGKRCCQSYDNFSYRSRSFRRSHRQMSCVNKESQYGFTPGNGQMPRGLEETITF
  YEVEEGDETAYPTLPNHGGPSTMVPATSGYCVGRRGHSSGKQTLNLEEGNGQSENGRYHEEYLYRAEPDY
  ETSGVYSTTASTANLSLQDRKSCSMSPQDTVTSYNYPQKMMGNIAAVAASCANNVPAPVLSNGAAANQAI
  STTSVSSQNAIQPLFVSPPTHGRPDTKVLQYYFNLGLQCYYHSYWHSMVYVPQMQQQLHVENYPVYTEPP
  LVDQTVPQCYSEVRREDGIQAEASANDTFPNADSSSVPHGAVYYPVMSDPYGQPPLPGFDSCLPVVPDYS
  CVPPWHPVGTAYGGSSQIHGAINPGPIGCIAPSPPASHYVPQGM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001257237
• ORF Size: 2862 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001257237.1, NP_001244166.1
• RefSeq Size: 4015
• RefSeq ORF: 2865
• Locus ID: 79868
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 106.9 kDa
• Gene Summary: The protein encoded by this gene is a subunit of a bipartite UDP-N-acetylglucosamine transferase. It heterodimerizes with asparagine-linked glycosylation 14 homolog to form a functional UDP-GlcNAc glycosyltransferase that catalyzes the second sugar addition of the highly conserved oligosaccharide precursor in endoplasmic reticulum N-linked glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]