AP4E1 (NM_001252127) Human Tagged ORF Clone

CAT#: RC233195

AP4E1 (Myc-DDK tagged) - Homo sapiens adaptor-related protein complex 4, epsilon 1 subunit (AP4E1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP4E1
• Synonyms: CPSQ4; SPG51; STUT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233195 representing NM_001252127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAGGAATGTATGGTGAGACTTATATATTGTGAAATGCTTGGATATGATGCTTCCTTTGGCTATA
  TTCATGCAATCAAGTTAGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTAGGTTATTTGGCTGTTTCCTT
  ATTTCTACATGAAAGTCATGAATTATTGCTTCTCCTTGTGAATACAGTTGTAAAGGATCTGCAGAGCACT
  AACCTAGTAGAAGTGTGTATGGCACTGACTGTTGTTAGCCAGATTTTCCCCTGCGAAATGATTCCAGCTG
  TTCTTCCATTAATAGAAGATAAACTTCAACATTCTAAGGAGATTGTACGAAGAAAAGCTGTTCTGGCATT
  ATACAAATTCCATCTCATTGCTCCTAATCAAGTACAACATATTCATATTAAGTTTCGGAAAGCACTTTGT
  GACAGAGATGTTGGGGTCATGGCTGCCTCCTTGCATATATATCTTAGAATGATTAAGGAGAATTCATCTG
  GATATAAAGACTTGACTGGGAGTTTTGTAACCATTTTGAAGCAAGTAGTTGGAGGAAAGCTCCCAGTAGA
  ATTCAATTACCACAGTGTGCCAGCACCATGGTTACAAATTCAGCTCTTGAGAATACTGGGACTTCTAGGA
  AAAGATGATCAAAGGACAAGTGAATTAATGTATGATGTTCTTGATGAATCCTTACGAAGAGCTGAGTTAA
  ATCACAATGTCACATATGCTATTTTGTTTGAATGTGTGCATACAGTCTATTCTATTTATCCTAAATCGGA
  ATTACTTGAGAAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATAAATCTAAAATATTTA
  GGACTGAAGGCTCTTACCTATGTTATCCAACAGGATCCTACTCTGGCTCTTCAACACCAGATGACAATAA
  TTGAATGTTTAGATCATCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCTTTACAGAATTACTAA
  TGCACAGAATATAACAGTTATTGTCCAGAAAATGCTTGAATATTTACATCAGAGCAAAGAAGAGTATGTC
  ATCGTCAATTTGGTCGGCAAAATAGCAGAGCTGGCTGAGAAATATGCTCCTGATAATGCATGGTTTATTC
  AGACAATGAATGCTGTGTTTTCAGTAGGAGGAGATGTAATGCATCCTGATATTCCCAATAACTTTCTGAG
  ACTACTAGCGGAAGGTTTTGATGATGAAACAGAAGATCAGCAATTAAGACTCTATGCAGTTCAGTCTTAT
  CTCACTTTACTGGATATGGAAAATGTGTTCTATCCACAGAGATTTCTTCAAGTTATGAGTTGGGTATTAG
  GGGAATATTCCTACCTCTTAGATAAGGAAACGCCAGAGGAAGTTATAGCTAAGCTCTACAAGTTACTTAT
  GAATGACTCTGTGTCTTCAGAAACAAAAGCCTGGTTAATTGCTGCTGTGACCAAATTGACATCTCAGGCG
  CACTCTTCTAATACAGTTGAGAGATTAATCCATGAATTTACCATATCTTTGGATACTTGTATGAGACAAC
  ATGCATTTGAATTAAAACATTTGCATGAGAATGTGGAACTTATGAAGAGCTTGCTTCCAGTTGACAGGAG
  TTGTGAAGACTTGGTGGTAGATGCTTCTTTATCTTTTCTGGATGGTTTTGTGGCTGAAGGACTCAGTCAG
  GGTGCAGCGCCTTACAAACCTCCCCATCAACGCCAGGAGGAAAAGCTTTCTCAGGAAAAAGTTCTCAATT
  TTGAACCATATGGACTCTCCTTTTCTTCATCTGGCTTCACTGGACGACAGTCTCCTGCTGGCATTTCTCT
  TGGTTCAGATGTATCTGGGAATAGTGCTGAGACAGGACTGAAAGAGACAAATAGCTTGAAGCTGGAAGGT
  ATAAAGAAATTGTGGGGGAAAGAAGGCTATCTTCCCAAGAAGGAAAGCAAAACTGGTGATGAAAGTGGAG
  CTCTGCCTGTTCCTCAAGAGAGTATAATGGAGAATGTAGATCAAGCTATAACTAAAAAGGATCAATCTCA
  AGTTCTTACCCAATCTAAAGAGGAGAAAGAAAAGCAGCTGCTGGCATCATCATTATTTGTTGGTCTAGGA
  TCAGAAAGTACAATCAACCTGCTGGGAAAAGCAGATACTGTCTCTCACAAGTTCAGAAGGAAATCAAAAG
  TCAAAGAAGCTAAAAGTGGCGAAACAACCAGTACTCATAATATGACCTGTTCTTCCTTTAGTTCTTTGTC
  AAATGTGGCATATGAAGATGATTATTATTCGAATACTTTGCACGATACAGGAGACAAGGAATTAAAGAAA
  TTTTCTCTCACTTCAGAACTTTTGGATTCTGAGTCACTCACAGAACTGCCCTTGGTTGAGAAATTCTCAT
  ATTGTAGTCTGTCTACACCTTCATTGTTTGCTAATAACAACATGGAAATTTTTCACCCTCCTCAATCTAC
  TGCAGCCTCAGTTGCCAAGGAAAGCTCTTTAGCTTCATCTTTTTTGGAAGAAACTACTGAATACATACAC
  TCAAATGCTATGGAAGTCTGTAATAATGAAACTATATCAGTGTCTTCTTATAAAATTTGGAAAGATGATT
  GTTTATTGATGGTCTGGTCAGTCACTAATAAGAGTGGTTTGGAATTGAAAAGTGCTGACTTAGAAATTTT
  TCCTGCAGAAAATTTCAAGGTGACTGAGCAACCTGGATGCTGTTTGCCTGTAATGGAAGCAGAAAGCACC
  AAAAGCTTTCAATATAGTGTGCAGATAGAAAAACCTTTTACAGAAGGAAATCTTACTGGTTTTATTAGTT
  ATCATATGATGGATACTCATTCTGCTCAGCTGGAATTTTCTGTAAACTTATCACTATTAGATTTCATTAG
  ACCATTAAAAATCTCAAGTGACGACTTTGGGAAACTCTGGTTATCCTTCGCAAATGATGTGAAACAAAAT
  GTAAAAATGTCAGAATCTCAAGCTGCACTTCCTTCTGCACTAAAGACTCTGCAACAGAAACTAAGACTCC
  ATATTATTGAGATTATAGGCAATGAAGGGCTATTGGCCTGTCAGCTGCTCCCATCCATCCCCTGCTTACT
  GCATTGCCGAGTTCATGCAGATGTATTAGCCCTGTGGTTCAGATCCTCCTGTTCTACTCTTCCTGACTAT
  TTACTGTATCAGTGTCAAAAGGTGATGGAGGGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233195 representing NM_001252127
  Red=Cloning site Green=Tags(s)
  
  MMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQST
  NLVEVCMALTVVSQIFPCEMIPAVLPLIEDKLQHSKEIVRRKAVLALYKFHLIAPNQVQHIHIKFRKALC
  DRDVGVMAASLHIYLRMIKENSSGYKDLTGSFVTILKQVVGGKLPVEFNYHSVPAPWLQIQLLRILGLLG
  KDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYL
  GLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNITVIVQKMLEYLHQSKEEYV
  IVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLYAVQSY
  LTLLDMENVFYPQRFLQVMSWVLGEYSYLLDKETPEEVIAKLYKLLMNDSVSSETKAWLIAAVTKLTSQA
  HSSNTVERLIHEFTISLDTCMRQHAFELKHLHENVELMKSLLPVDRSCEDLVVDASLSFLDGFVAEGLSQ
  GAAPYKPPHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDVSGNSAETGLKETNSLKLEG
  IKKLWGKEGYLPKKESKTGDESGALPVPQESIMENVDQAITKKDQSQVLTQSKEEKEKQLLASSLFVGLG
  SESTINLLGKADTVSHKFRRKSKVKEAKSGETTSTHNMTCSSFSSLSNVAYEDDYYSNTLHDTGDKELKK
  FSLTSELLDSESLTELPLVEKFSYCSLSTPSLFANNNMEIFHPPQSTAASVAKESSLASSFLEETTEYIH
  SNAMEVCNNETISVSSYKIWKDDCLLMVWSVTNKSGLELKSADLEIFPAENFKVTEQPGCCLPVMEAEST
  KSFQYSVQIEKPFTEGNLTGFISYHMMDTHSAQLEFSVNLSLLDFIRPLKISSDDFGKLWLSFANDVKQN
  VKMSESQAALPSALKTLQQKLRLHIIEIIGNEGLLACQLLPSIPCLLHCRVHADVLALWFRSSCSTLPDY
  LLYQCQKVMEGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252127
• ORF Size: 3186 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001252127.1, NP_001239056.1
• RefSeq Size: 6791
• RefSeq ORF: 3189
• Locus ID: 23431
• Protein Pathways: Lysosome
• MW: 119.9 kDa
• Gene Summary: This gene encodes a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and sorting of integral membrane proteins. The encoded protein is a large subunit of adaptor protein complex-4, which is associated with both clathrin- and nonclathrin-coated vesicles. Disruption of this gene may be associated with cerebral palsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]