UBE4A (NM_001204077) Human Tagged ORF Clone

CAT#: RC233197

UBE4A (Myc-DDK tagged) - Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBE4A
• Synonyms: E4; UBOX2; UFD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233197 representing NM_001204077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTG
  ATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGCCC
  AGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGCCGC
  TCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTA
  TTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGA
  AGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCT
  CGCTTATTACTTCAAGATCCAGGCAACCACTTAATTAACATGACTTCTTCTACAACGCTAAATCTCTCTG
  CTGATCGAGATGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAGCCAAGGAAGA
  GATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAATACC
  CGAACAGTTCTTCTCACCCCAGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGT
  TAGAAGCCATCCAGGGAGCCCATTTTGAAGATGTAACTGAGTTTCTGGAAGAGGTCATTGAAGCCTTGAT
  ATTGGATGAGGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTTGATATTTTATTGGGCCGAATA
  AAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATATCTGGATATTCTTCTCTATTTCACTAGGCAAA
  AAGATATGGCAAAGGTTTTTGTAGAATACATTCAGCCCAAGGACCCTACCAATGGGCAAATGTACCAGAA
  GACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCGGGTGTTGTAGAAAATCATGGC
  TACTTTTTGAATCCATCTCGTTCCAGCCCCCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCA
  TGGCTCAGTTCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCAGCTCTCTCCAGAAACCAAACA
  CTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAATGCAGGCCGCACCAAGATTTGGGCCAATCAG
  ATGCCAGAAATCTTTTTCCAAATGTATGCCTCAGATGCTTTCTTTCTGAATCTGGGTGCTGCTCTCCTGA
  AGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTTTAATCCCACATACTGTGCCCT
  CAAGGAGTTGAATGATGAAGAACGAAAAATTAAAAATGTACACATGAGAGGTTTGGACAAAGAAACCTGT
  TTGATCCCAGCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTTGTAACAGAGAACCTTGCTCTGA
  CAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCA
  TCGGCTGCAGGTTGCCTGGCGGGATGCTCAGCAAAGTTCTAGCCCTGCTGCTGACAATCTTCGTGAGCAG
  TTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAGAGCCACAAATGCTACAAAACT
  GCCTAAACTTGCAGGTGTCCATGGCTGTTCTACTGGTTCAACTGGCCATAGGCAATGAGGGCTCACAGCC
  AATAGAGCTAACCTTTCCTTTGCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAATTTTTTGCAGAT
  AACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCCGATGACATTTTGGAGACATCAGCAGATTCCC
  TGGAGCATGTCCTTCACTTTATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGAG
  GGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAGACCCCAAATCCCTTGGTATCC
  AGTGTGTTCCACCGGAAACGTGTGTTCTGCAACTTTCAGTATGCACCCCAACTTGCAGAGGCTCTAATCA
  AGGTTTTTGTGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACAGAAGTTTAATTACCGCCGTCC
  CATGTATCCTATCCTAAGATACATGTGGGGGACAGATACCTATCGGGAGAGCATTAAGGATTTGGCTGAC
  TATGCCTCTAAGAATTTAGAAGCCATGAATCCCCCACTTTTCCTCCGCTTTCTTAACCTGCTAATGAATG
  ATGCCATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAAGATTCAGCAAATTGAGAAGGA
  TCGAGGTGAATGGGATAGTCTGACTCCAGAAGCCCGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGA
  CAGCTGGCACGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCCTTGCCTTTCTCACATCAGAGA
  TCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCATCATCTCCATGTTGAACTACTTCCTGCAACA
  CCTGGTTGGCCCCAAGATGGGTGCCTTAAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCCCAGCAG
  CTTGTATCAGATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGAATTTCTGTGCCACTGTGCCCA
  AGGATGGACGTTCCTATTCCCCAACTCTCTTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCC
  TGGGAATATGATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACCTCCAACAACAG
  GAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTGGATCCCATTATGAGCACACTGATGTGTGACC
  CTGTGGTGCTGCCATCTTCCAGAGTCACTGTGGATAGATCCACCATTGCAAGACATTTGCTCAGTGACCA
  AACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCAAACACAGAACTAAAAGAAAAA
  ATCCAACGGTGGCTTGCAGAGAGGAAACAACAAAAGGAGCAACTTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233197 representing NM_001204077
  Red=Cloning site Green=Tags(s)
  
  MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISR
  SFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFA
  RLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNT
  RTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDILLGRI
  KDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHG
  YFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQ
  MPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETC
  LIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQ
  FERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFAD
  NLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVS
  SVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLAD
  YASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFG
  QLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQ
  LVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKSLADLQQQ
  EEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEK
  IQRWLAERKQQKEQLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204077
• ORF Size: 3198 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001204077.1, NP_001191006.1
• RefSeq Size: 6123
• RefSeq ORF: 3201
• Locus ID: 9354
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 123 kDa
• Gene Summary: This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]