IST1 (NM_001270975) Human Tagged ORF Clone

CAT#: RC233891

IST1 (Myc-DDK tagged) - Homo sapiens increased sodium tolerance 1 homolog (yeast) (IST1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IST1
• Synonyms: CHMP8; OLC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233891 representing NM_001270975
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCTCTGGATTTAAAGCTGAGCGCTTAAGAGTGAATTTGAGATTAGTCATAAATCGCCTTAAAC
  TATTGGAGAAAAAGAAAACGGAACTGGCCCAGAAAGCAAGGAAGGAGATTGCTGACTATCTGGCTGCTGG
  GAAAGATGAACGAGCTCGGATCCGTGTGGAGCACATTATCCGGGAAGACTACCTCGTGGAGGCCATGGAG
  ATCCTGGAGCTGTACTGTGACCTGCTGCTGGCTCGGTTTGGCCTTATCCAGTCTATGAAGGAACTAGATT
  CTGGTCTGGCTGAATCTGTGTCTACATTGATCTGGGCTGCTCCTCGACTCCAGTCAGAAGTGGCTGAGTT
  GAAAATAGTTGCTGATCAGCTCTGTGCCAAGTATAGCAAGGAATATGGCAAGCTATGTAGGACCAACCAG
  ATTGGAACTGTGAATGACAGGCTAATGCACAAGCTGAGTGTGGAAGCCCCACCCAAAATCCTGGTGGAGA
  GATACCTGATTGAAATTGCAAAGAATTACAACGTACCCTATGAACCTGACTCTGTGGTCATGGCAGAAGC
  TCCTCCTGGGGTAGAGACAGATCTTATTGATGTTGGATTCACAGATGATGTGAAGAAAGGAGGCCCTGGA
  AGAGGAGGGAGTGGTGGCTTCACAGCACCAGTTGGTGGACCTGATGGAACGGTGCCAATGCCCATGCCCA
  TGCCCATGCCTATGCCATCTGCAAATACGCCTTTCTCATATCCACTGCCAAAGGGACCATCAGATTTCAA
  TGGACTGCCAATGGGGACTTATCAGGCCTTTCCCAATATTCATCCACCTCAGATACCAGCAACTCCCCCA
  TCGTATGAATCTGTAGATGACATTAATGCTGATAAGAATATCTCTTCTGCACAGATTGTTGGTCCTGGAC
  CCAAGCCAGAAGCCTCTGCAAAGCTTCCTTCCAGACCTGCAGATAACTATGACAACTTTGTCCTACCAGA
  GTTGCCATCTGTGCCAGACACACTACCAACTGCATCTGCTGGTGCCAGCACCTCAGCATCTGAAGACATT
  GACTTTGATGATCTTTCCCGGAGGTTTGAAGAGCTGAAAAAGAAAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233891 representing NM_001270975
  Red=Cloning site Green=Tags(s)
  
  MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAME
  ILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQ
  IGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPG
  RGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPP
  SYESVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDI
  DFDDLSRRFEELKKKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270975
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270975.1, NP_001257904.1
• RefSeq Size: 4039
• RefSeq ORF: 1101
• Locus ID: 9798
• MW: 40.4 kDa
• Gene Summary: This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]