Corticotropin Releasing Factor Receptor 2 (CRHR2) (NM_001202483) Human Tagged ORF Clone

CAT#: RC233948

CRHR2 (Myc-DDK tagged) - Homo sapiens corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRHR2
• Synonyms: CRF-RB; CRF2; CRFR2; HM-CRF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233948 representing NM_001202483
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGGCACTGCTCCACAGCCTGCTGGAGGCCAACTGCAGCCTGGCGCTGGCTGAAGAGCTGCTCT
  TGGACGGCTGGGGGCCACCCCTGGACCCCGAGGGTCCCTACTCCTACTGCAACACGACCTTGGACCAGAT
  CGGAACGTGCTGGCCCCGCAGCGCTGCCGGAGCCCTCGTGGAGAGGCCGTGCCCCGAGTACTTCAACGGC
  GTCAAGTACAACACGACCCGGAATGCCTATCGAGAATGCTTGGAGAATGGGACGTGGGCCTCAAAGATCA
  ACTACTCACAGTGTGAGCCCATTTTGGATGACAAGCAGAGGAAGTATGACCTGCACTACCGCATCGCCCT
  TGTCGTCAACTACCTGGGCCACTGCGTATCTGTGGCAGCCCTGGTGGCCGCCTTCCTGCTTTTCCTGGCC
  CTGCGGAGCATTCGCTGTCTGCGGAATGTGATTCACTGGAACCTCATCACCACCTTTATCCTGCGAAATG
  TCATGTGGTTCCTGCTGCAGCTCGTTGACCATGAAGTGCACGAGAGCAATGAGGTCTGGTGCCGCTGCAT
  CACCACCATCTTCAACTACTTCGTGGTGACCAACTTCTTCTGGATGTTTGTGGAAGGCTGCTACCTGCAC
  ACGGCCATTGTCATGACCTACTCCACTGAGCGCCTGCGCAAGTGCCTCTTCCTCTTCATCGGATGGTGCA
  TCCCCTTCCCCATCATCGTCGCCTGGGCCATCGGCAAGCTCTACTATGAGAATGAACAGTGCTGGTTTGG
  CAAGGAGCCTGGCGACCTGGTGGACTACATCTACCAAGGCCCCATCATTCTCGTGCTCCTGATCAATTTC
  GTATTTCTGTTCAACATCGTCAGGATCCTAATGACAAAGTTACGCGCGTCCACCACATCCGAGACAATCC
  AGTACAGGAAGGCAGTGAAGGCCACCCTGGTGCTCCTGCCCCTCCTGGGCATCACCTACATGCTCTTCTT
  CGTCAATCCCGGGGAGGACGACCTGTCACAGATCATGTTCATCTATTTCAACTCCTTCCTGCAGTCGTTC
  CAGGGTTTCTTCGTGTCTGTCTTCTACTGCTTCTTCAATGGAGAGAGCTGGGTGAGCAAGGAAGCCCAGG
  CCGCAGGGCCTCATGGAAGAGAGAAGCCAGAGCAGAGGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233948 representing NM_001202483
  Red=Cloning site Green=Tags(s)
  
  MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNG
  VKYNTTRNAYRECLENGTWASKINYSQCEPILDDKQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLA
  LRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESNEVWCRCITTIFNYFVVTNFFWMFVEGCYLH
  TAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQGPIILVLLINF
  VFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSF
  QGFFVSVFYCFFNGESWVSKEAQAAGPHGREKPEQRW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001202483
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202483.1, NP_001189412.1
• RefSeq Size: 3109 bp
• RefSeq ORF: 1164 bp
• Locus ID: 1395
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 45.3 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jan 2011]'