Synaptotagmin VI (SYT6) (NM_001270805) Human Tagged ORF Clone
CAT#: RC234061
- TrueORF®
SYT6 (Myc-DDK tagged) - Homo sapiens synaptotagmin VI (SYT6), transcript variant 3
"NM_001270805" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SYT6 |
Synonyms | sytVI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234061 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGAGGAACAAGGAGGCCTCCAGTCCCTCTTCTGCTAATCCCCCCTTGGAAGCCCTCCAGAGCC CCAGCTTCAGAGGCAACATGGCGGACAAGCTGAAGGACCCCAGCACCCTGGGCTTCCTGGAGGCGGCCGT GAAGATCAGCCACACGTCCCCAGATATCCCAGCTGAGGTGCAGATGTCGGTCAAGGAGCACATCATGCGT CACACCCGGCTGCAGCGGCAAACTACAGAGCCAGCGTCATCCACCAGGCACACGTCCTTCAAGCGCCACC TGCCAAGGCAGATGCATGTCTCCAGTGTAGACTATGGCAATGAGCTTCCACCAGCAGCAGAGCAGCCCAC CAGCATTGGCCGCATCAAGCCTGAGCTCTACAAGCAGAAGTCGGTGGATGGGGAGGATGCCAAGTCTGAG GCCACCAAGAGCTGCGGGAAGATCAACTTCAGCCTACGCTACGATTACGAGACCGAGACCCTGATTGTGC GTATCCTGAAGGCTTTTGACCTCCCTGCCAAGGACTTTTGTGGAAGCTCTGACCCTTATGTCAAGATCTA CCTCCTGCCTGACCGCAAATGCAAGCTGCAGACCCGGGTGCACCGCAAGACCCTGAACCCCACCTTTGAT GAGAACTTCCACTTCCCTGTGCCCTATGAGGAGCTGGCTGACCGCAAGCTGCATCTCAGTGTCTTCGACT TTGACCGCTTCTCCCGCCATGACATGATTGGCGAGGTCATCCTGGACAACCTCTTTGAGGCCTCTGACCT GTCTCGGGAAACCTCCATCTGGAAGGATATCCAATATGCCACAAGTGAAAGCGTGGACTTGGGAGAGATC ATGTTCTCCCTTTGCTACCTGCCCACTGCAGGCAGGCTCACCCTCACAGTGATTAAGTGTCGGAACCTCA AGGCGATGGACATCACAGGCTATTCAGATCCCTATGTGAAAGTGTCCTTGCTCTGTGATGGGCGGAGGCT GAAGAAGAAGAAAACAACCATAAAGAAAAACACTCTCAATCCTGTCTACAATGAGGCCATCATCTTTGAC ATTCCCCCGGAAAACATGGATCAAGTCAGCCTGCTCATCTCAGTCATGGACTATGATCGAGTGGGCCACA ATGAGATCATAGGAGTCTGTCGTGTGGGGATCACTGCTGAAGGCCTGGGCAGGGACCACTGGAACGAGAT GCTGGCATACCCCCGGAAGCCCATCGCACACTGGCACTCCTTGGTGGAGGTAAAGAAATCCTTCAAAGAG GGAAACCCTCGGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234061 protein sequence
Red=Cloning site Green=Tags(s) MPWRNKEASSPSSANPPLEALQSPSFRGNMADKLKDPSTLGFLEAAVKISHTSPDIPAEVQMSVKEHIMR HTRLQRQTTEPASSTRHTSFKRHLPRQMHVSSVDYGNELPPAAEQPTSIGRIKPELYKQKSVDGEDAKSE ATKSCGKINFSLRYDYETETLIVRILKAFDLPAKDFCGSSDPYVKIYLLPDRKCKLQTRVHRKTLNPTFD ENFHFPVPYEELADRKLHLSVFDFDRFSRHDMIGEVILDNLFEASDLSRETSIWKDIQYATSESVDLGEI MFSLCYLPTAGRLTLTVIKCRNLKAMDITGYSDPYVKVSLLCDGRRLKKKKTTIKKNTLNPVYNEAIIFD IPPENMDQVSLLISVMDYDRVGHNEIIGVCRVGITAEGLGRDHWNEMLAYPRKPIAHWHSLVEVKKSFKE GNPRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001270805 |
ORF Size | 1275 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001270805.1, NP_001257734.1 |
RefSeq Size | 4375 bp |
RefSeq ORF | 1278 bp |
Locus ID | 148281 |
Protein Families | Secreted Protein, Transmembrane |
MW | 48.4 kDa |
Gene Summary | The protein encoded by this gene belongs to the synaptotagmin family. Synaptotagmins share a common domain structure that includes a transmembrane domain and a cytoplasmic region composed of 2 C2 domains, and are involved in calcium-dependent exocytosis of synaptic vesicles. This protein has been shown to be a key component of the secretory machinery involved in acrosomal exocytosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2011] |
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