Synaptotagmin VI (SYT6) (NM_001270805) Human Tagged ORF Clone

CAT#: RC234061

SYT6 (Myc-DDK tagged) - Homo sapiens synaptotagmin VI (SYT6), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SYT6
• Synonyms: sytVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234061 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGGAGGAACAAGGAGGCCTCCAGTCCCTCTTCTGCTAATCCCCCCTTGGAAGCCCTCCAGAGCC
  CCAGCTTCAGAGGCAACATGGCGGACAAGCTGAAGGACCCCAGCACCCTGGGCTTCCTGGAGGCGGCCGT
  GAAGATCAGCCACACGTCCCCAGATATCCCAGCTGAGGTGCAGATGTCGGTCAAGGAGCACATCATGCGT
  CACACCCGGCTGCAGCGGCAAACTACAGAGCCAGCGTCATCCACCAGGCACACGTCCTTCAAGCGCCACC
  TGCCAAGGCAGATGCATGTCTCCAGTGTAGACTATGGCAATGAGCTTCCACCAGCAGCAGAGCAGCCCAC
  CAGCATTGGCCGCATCAAGCCTGAGCTCTACAAGCAGAAGTCGGTGGATGGGGAGGATGCCAAGTCTGAG
  GCCACCAAGAGCTGCGGGAAGATCAACTTCAGCCTACGCTACGATTACGAGACCGAGACCCTGATTGTGC
  GTATCCTGAAGGCTTTTGACCTCCCTGCCAAGGACTTTTGTGGAAGCTCTGACCCTTATGTCAAGATCTA
  CCTCCTGCCTGACCGCAAATGCAAGCTGCAGACCCGGGTGCACCGCAAGACCCTGAACCCCACCTTTGAT
  GAGAACTTCCACTTCCCTGTGCCCTATGAGGAGCTGGCTGACCGCAAGCTGCATCTCAGTGTCTTCGACT
  TTGACCGCTTCTCCCGCCATGACATGATTGGCGAGGTCATCCTGGACAACCTCTTTGAGGCCTCTGACCT
  GTCTCGGGAAACCTCCATCTGGAAGGATATCCAATATGCCACAAGTGAAAGCGTGGACTTGGGAGAGATC
  ATGTTCTCCCTTTGCTACCTGCCCACTGCAGGCAGGCTCACCCTCACAGTGATTAAGTGTCGGAACCTCA
  AGGCGATGGACATCACAGGCTATTCAGATCCCTATGTGAAAGTGTCCTTGCTCTGTGATGGGCGGAGGCT
  GAAGAAGAAGAAAACAACCATAAAGAAAAACACTCTCAATCCTGTCTACAATGAGGCCATCATCTTTGAC
  ATTCCCCCGGAAAACATGGATCAAGTCAGCCTGCTCATCTCAGTCATGGACTATGATCGAGTGGGCCACA
  ATGAGATCATAGGAGTCTGTCGTGTGGGGATCACTGCTGAAGGCCTGGGCAGGGACCACTGGAACGAGAT
  GCTGGCATACCCCCGGAAGCCCATCGCACACTGGCACTCCTTGGTGGAGGTAAAGAAATCCTTCAAAGAG
  GGAAACCCTCGGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234061 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPWRNKEASSPSSANPPLEALQSPSFRGNMADKLKDPSTLGFLEAAVKISHTSPDIPAEVQMSVKEHIMR
  HTRLQRQTTEPASSTRHTSFKRHLPRQMHVSSVDYGNELPPAAEQPTSIGRIKPELYKQKSVDGEDAKSE
  ATKSCGKINFSLRYDYETETLIVRILKAFDLPAKDFCGSSDPYVKIYLLPDRKCKLQTRVHRKTLNPTFD
  ENFHFPVPYEELADRKLHLSVFDFDRFSRHDMIGEVILDNLFEASDLSRETSIWKDIQYATSESVDLGEI
  MFSLCYLPTAGRLTLTVIKCRNLKAMDITGYSDPYVKVSLLCDGRRLKKKKTTIKKNTLNPVYNEAIIFD
  IPPENMDQVSLLISVMDYDRVGHNEIIGVCRVGITAEGLGRDHWNEMLAYPRKPIAHWHSLVEVKKSFKE
  GNPRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270805
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270805.1, NP_001257734.1
• RefSeq Size: 4375 bp
• RefSeq ORF: 1278 bp
• Locus ID: 148281
• Protein Families: Secreted Protein, Transmembrane
• MW: 48.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the synaptotagmin family. Synaptotagmins share a common domain structure that includes a transmembrane domain and a cytoplasmic region composed of 2 C2 domains, and are involved in calcium-dependent exocytosis of synaptic vesicles. This protein has been shown to be a key component of the secretory machinery involved in acrosomal exocytosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2011]