Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (NM_001256573) Human Tagged ORF Clone

CAT#: RC234138

  • TrueORF®

CHRNA4 (Myc-DDK tagged) - Homo sapiens cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), transcript variant 2


  "NM_001256573" in other vectors (2)

Reconstitution Protocol

USD 470.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CHRNA4
Synonyms BFNC; EBN; EBN1; NACHR; NACHRA4; NACRA4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC234138 representing NM_001256573
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAATTCGGCTCCTGGACCTACGACAAGGCCAAGATCGACCTGGTGAACATGCACAGCCGCGTGGACC
AGCTGGACTTCTGGGAGAGTGGCGAGTGGGTCATCGTGGATGCCGTGGGCACCTACAACACCAGGAAGTA
CGAGTGCTGTGCCGAGATCTACCCGGACATCACCTATGCCTTCGTCATCCGGCGGCTGCCGCTCTTCTAC
ACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTGCCTCACCGTGCTGGTCTTCTACCTGCCCTCCG
AGTGTGGCGAGAAGATCACGCTGTGCATCTCCGTGCTGCTGTCGCTCACCGTCTTCCTGCTGCTCATCAC
CGAGATCATCCCGTCCACCTCACTGGTCATCCCACTCATCGGCGAGTACCTGCTGTTCACCATGATCTTC
GTCACCCTGTCCATCGTCATCACGGTCTTCGTGCTCAACGTGCACCACCGCTCGCCACGCACGCACACCA
TGCCCACCTGGGTACGCAGGGTCTTCCTGGACATCGTGCCACGCCTGCTCCTCATGAAGCGGCCGTCCGT
GGTCAAGGACAATTGCCGGCGGCTCATCGAGTCCATGCATAAGATGGCCAGTGCCCCGCGCTTCTGGCCC
GAGCCAGAAGGGGAGCCCCCTGCCACGAGCGGCACCCAGAGCCTGCACCCGCCCTCACCGTCCTTCTGTG
TCCCCCTGGATGTGCCGGCTGAGCCTGGGCCTTCCTGCAAGTCACCCTCCGACCAGCTCCCTCCTCAGCA
GCCCCTGGAAGCTGAGAAAGCCAGCCCCCACCCCTCGCCTGGACCCTGCCGCCCGCCCCACGGCACCCAG
GCACCAGGGCTGGCCAAAGCCAGGTCCCTCAGCGTCCAGCACATGTCCAGCCCTGGCGAAGCGGTGGAAG
GCGGCGTCCGGTGCCGGTCTCGGAGCATCCAGTACTGTGTTCCCCGAGACGATGCCGCCCCCGAGGCAGA
TGGCCAGGCTGCCGGCGCCCTGGCCTCTCGCAACACCCACTCGGCTGAGCTCCCACCCCCAGACCAGCCC
TCTCCGTGCAAATGCACATGCAAGAAGGAGCCCTCTTCGGTGTCCCCGAGCGCCACGGTCAAGACCCGCA
GCACCAAAGCGCCGCCCCCGCACCTGCCCCTGTCGCCGGCCCTGACCCGGGCGGTGGAGGGCGTCCAGTA
CATTGCAGACCACCTGAAGGCCGAAGACACAGACTTCTCGGTGAAGGAGGACTGGAAGTACGTGGCCATG
GTCATCGACCGCATCTTCCTCTGGATGTTCATCATCGTCTGCCTGCTGGGGACGGTGGGCCTCTTCCTGC
CGCCCTGGCTGGCTGGCATGATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC234138 representing NM_001256573
Red=Cloning site Green=Tags(s)

MKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFY
TINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIF
VTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWP
EPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQ
APGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQP
SPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAM
VIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001256573
ORF Size 1353 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001256573.1, NP_001243502.1
RefSeq Size 5474 bp
RefSeq ORF 1356 bp
Locus ID 1137
Cytogenetics 20q13.33
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
MW 50.3 kDa
Gene Summary 'This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.