B4GALNT1 (NM_001276468) Human Tagged ORF Clone

CAT#: RC234220

B4GALNT1 (Myc-DDK tagged) - Homo sapiens beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4GALNT1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B4GALNT1
• Synonyms: GALGT; GalNAc-T; GALNACT; SPG26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234220 representing NM_001276468
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGGGCCGCCGGGCCCTGTGCGCTCTGGTCCTTCTGCTCGCCTGCGCCTCGCTGGGGCTCCTGT
  ACGCGAGCACCCGGGACGCGCCCGGCCTCCGGCTACCTCTTGCGCCGTGGGCGCCCCCGCAAAGCCCCCG
  CAGGCCCGAGCTGCCAGATCTTGCTCCTGAGCCCCGCTACGCACACATCCCGGTCAGGATCAAGGAGCAA
  GTAGTGGGGAGCCAGTCCCCAGCTGACCAGCTGCTCATAGCCCCTGCCAACTCCCCGCTCCAGTACCCCC
  TACAGGGTGTGGAAGTTCAGCCCCTCAGGAGCATCTTGGTGCCAGGGCTGAGCCTTCAGGCAGCTTCTGG
  TCAGGAGGTATACCAGGTGAACCTGACTGCCTCCCTAGGCACCTGGGACGTGGCAGGGGAAGTGACTGGA
  GTTACTCTCACTGGAGAGGGTCAGGCAGATCTCACCCTTGTCAGCCCAGGGCTGGACCAACTCAACAGGC
  AACTACAACTGGTCACTTACAGCAGCCGAAGCTACCAGACCAACACAGCAGACACAGTCCGGTTCTCCAC
  CGAGGGACATGAGGCTGCTTTCACTATCCGCATAAGACACCCGCCCAACCCTCGGCTGTACCCACCTGGG
  TCTCTACCCCAGGGAGCCCAGTACAACATCAGCGCTCTAGTCACGATTGCCACCAAGACCTTCCTCCGTT
  ATGATCGGCTACGGGCTCTCATCACCAGTATCCGCCGCTTCTACCCAACGGTTACCGTGGTCATCGCTGA
  CGACAGCGACAAGCCAGAGCGCGTTAGTGGCCCCTACGTGGAACACTATCTCATGCCCTTCGGCAAGGGC
  TGGTTCGCAGGCCGGAACCTGGCCGTGTCTCAAGTAACCACCAAGTACGTGCTGTGGGTGGACGACGACT
  TCGTCTTCACGGCGCGGACGCGGCTGGAGAGGCTTGTGGACGTGCTGGAGCGGACGCCGCTGGACCTGGT
  GGGGGGCGCGGTGCGCGAGATCTCCGGCTTTGCCACCACTTATCGGCAGCTGCTGAGCGTGGAGCCCGGC
  GCCCCAGGCCTCGGGAACTGCCTCCGGCAAAGGCGCGGCTTCCACCACGAGCTCGTCGGCTTCCCAGGCT
  GCGTGGTCACCGACGGCGTGGTTAACTTCTTCCTGGCGCGGACTGACAAGGTGCGCGAGGTCGGTTTCGA
  CCCCCGCCTCAGCCGCGTGGCTCATCTGGAATTCTTCTTGGATGGGCTTGGTTCCCTTCGGGTTGGCTCC
  TGCTCCGACGTCGTGGTGGATCATGCATCCAAACTGAAGCTGCCTTGGACATCAAGGGATGCCGGAGCAG
  AGACTTACGCCCGGTACCGTTACCCAGGATCACTGGACGAGAGCCAGATGGCCAAACACCGGCTGCTCTT
  CTTCAAACACCGGCTGCAGTGCATGACCTCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234220 representing NM_001276468
  Red=Cloning site Green=Tags(s)
  
  MWLGRRALCALVLLLACASLGLLYASTRDAPGLRLPLAPWAPPQSPRRPELPDLAPEPRYAHIPVRIKEQ
  VVGSQSPADQLLIAPANSPLQYPLQGVEVQPLRSILVPGLSLQAASGQEVYQVNLTASLGTWDVAGEVTG
  VTLTGEGQADLTLVSPGLDQLNRQLQLVTYSSRSYQTNTADTVRFSTEGHEAAFTIRIRHPPNPRLYPPG
  SLPQGAQYNISALVTIATKTFLRYDRLRALITSIRRFYPTVTVVIADDSDKPERVSGPYVEHYLMPFGKG
  WFAGRNLAVSQVTTKYVLWVDDDFVFTARTRLERLVDVLERTPLDLVGGAVREISGFATTYRQLLSVEPG
  APGLGNCLRQRRGFHHELVGFPGCVVTDGVVNFFLARTDKVREVGFDPRLSRVAHLEFFLDGLGSLRVGS
  CSDVVVDHASKLKLPWTSRDAGAETYARYRYPGSLDESQMAKHRLLFFKHRLQCMTSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276468
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276468.1, NP_001263397.1
• RefSeq Size: 2428 bp
• RefSeq ORF: 1437 bp
• Locus ID: 2583
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• MW: 53.3 kDa
• Gene Summary: 'GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]'