CHRNA6 (NM_001199279) Human Tagged ORF Clone

CAT#: RC234221

CHRNA6 (Myc-DDK tagged) - Homo sapiens cholinergic receptor, nicotinic, alpha 6 (neuronal) (CHRNA6), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHRNA6
• Synonyms: CHNRA6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234221 representing NM_001199279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACCAGCAAGGGGCAGGGATTCCTTCATGGGGGCTTGTGTCTCTGGCTGTGTGTGTTCACACCTT
  TCTTTAAAGGCTGTGTGGGCTGTGCAACTGAGGAGAGGCTCTTCCACAAACTGTTTTCTCATTACAACCA
  GTTCATCAGGCCTGTGGAAAACGTTTCCGACCCTGTCACGGTACACTTTGAAGTGGCCATCACCCAGCTG
  GCCAACGTGATCTGGAATGATTATAAATTGCGCTGGGATCCAATGGAATATGATGGCATTGAGACTCTTC
  GCGTTCCTGCAGATAAGATTTGGAAGCCCGACATTGTTCTCTATAACAATGCTGTTGGTGACTTCCAAGT
  AGAAGGCAAAACAAAAGCTCTTCTTAAATACAATGGCATGATAACCTGGACTCCACCAGCTATTTTTAAG
  AGTTCCTGCCCTATGGATATCACCTTTTTCCCTTTTGATCATCAAAACTGTTCCCTAAAATTTGGTTCCT
  GGACGTATGACAAAGCTGAAATTGATCTTCTAATCATTGGATCAAAAGTGGATATGAATGATTTTTGGGA
  AAACAGTGAATGGGAAATCATTGATGCCTCTGGCTACAAACATGACATCAAATACAACTGTTGTGAAGAG
  ATATACACAGATATAACCTATTCTTTCTACATTAGAAGATTGCCGATGTTTTACACGATTAATCTGATCA
  TCCCTTGTCTCTTTATTTCATTTCTAACCGTGTTGGTCTTTTACCTTCCTTCGGACTGTGGTGAAAAAGT
  GACGCTTTGTATTTCAGTCCTGCTTTCTCTGACTGTGTTTTTGCTGGTCATCACAGAAACCATCCCATCC
  ACATCTCTGGTGGTCCCACTGGTGGGTGAGTACCTGCTGTTCACCATGATCTTTGTCACACTGTCCATCG
  TGGTGACTGTGTTTGTGTTGAACATACACTACCGCACCCCAACCACGCACACAATGCCCAGGTGGGTGAA
  GACAGTTTTCCTGAAGCTGCTGCCCCAGGTCCTGCTGATGAGGTGGCCTCTGGACAAGACAAGGGGCACA
  GGCTCTGATGCAGTGCCCAGAGGCCTTGCCAGGAGGCCTGCCAAAGGCAAGCTTGCAAGCCATGGGGAAC
  CCAGACATCTTAAAGAATGCTTCCATTGTCACAAATCAAATGAGCTTGCCACAAGCAAGAGAAGATTAAG
  TCATCAGCCATTACAGTGGGTGGTGGAAAATTCGGAGCACTCGCCTGAAGTTGAAGATGTGATTAACAGT
  GTTCAGTTCATAGCAGAAAACATGAAGAGCCACAATGAAACCAAGGAGGTAGAAGATGACTGGAAATACG
  TGGCCATGGTGGTGGACAGAGTATTTCTTTGGGTATTTATAATTGTCTGTGTATTTGGAACTGCAGGGCT
  ATTTCTACAGCCACTACTTGGGAACACAGGAAAATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234221 representing NM_001199279
  Red=Cloning site Green=Tags(s)
  
  MLTSKGQGFLHGGLCLWLCVFTPFFKGCVGCATEERLFHKLFSHYNQFIRPVENVSDPVTVHFEVAITQL
  ANVIWNDYKLRWDPMEYDGIETLRVPADKIWKPDIVLYNNAVGDFQVEGKTKALLKYNGMITWTPPAIFK
  SSCPMDITFFPFDHQNCSLKFGSWTYDKAEIDLLIIGSKVDMNDFWENSEWEIIDASGYKHDIKYNCCEE
  IYTDITYSFYIRRLPMFYTINLIIPCLFISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPS
  TSLVVPLVGEYLLFTMIFVTLSIVVTVFVLNIHYRTPTTHTMPRWVKTVFLKLLPQVLLMRWPLDKTRGT
  GSDAVPRGLARRPAKGKLASHGEPRHLKECFHCHKSNELATSKRRLSHQPLQWVVENSEHSPEVEDVINS
  VQFIAENMKSHNETKEVEDDWKYVAMVVDRVFLWVFIIVCVFGTAGLFLQPLLGNTGKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199279
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001199279.1, NP_001186208.1
• RefSeq Size: 2355
• RefSeq ORF: 1440
• Locus ID: 8973
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• MW: 55.5 kDa
• Gene Summary: This gene encodes an alpha subunit of neuronal nicotinic acetylcholine receptors. These receptors consist of five subunits and function as ion channels involved in neurotransmission. The encoded protein is a subunit of neuronal nicotinic acetylcholine receptors that mediate dopaminergic neurotransmission and are activated by acetylcholine and exogenous nicotine. Alternatively spliced transcript variants have been observed for this gene. Single nucleotide polymorphisms in this gene have been associated with both nicotine and alcohol dependence. [provided by RefSeq, Dec 2010]