PDE1A (NM_001258313) Human Tagged ORF Clone
CAT#: RC234336
- TrueORF®
PDE1A (Myc-DDK tagged) - Homo sapiens phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 4
"NM_001258313" in other vectors (2)
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Other products for "PDE1A"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PDE1A |
Synonyms | CAM-PDE-1A; CAM-PDE 1A; HCAM-1; HCAM1; HSPDE1A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234336 representing NM_001258313
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACCATGTCACAATCAGGAAGAAACATCTCCAAAGACCCATCTTTAGACTAAGATGCTTGGTGA AGCAGCTGGAAAGAGGTGATGTTAACGTCGTCGACTTAAAGAAGAATATTGAATATGCGGCATCTGTGCT GGAAGCAGTTTATATCGATGAAACAAGAAGACTTCTGGATACTGAAGATGAGCTCAGTGACATTCAGACT GACTCAGTCCCATCTGAAGTCCGGGACTGGTTGGCTTCTACCTTTACACGGAAAATGGGGATGACAAAAA AGAAACCTGAGGAAAAACCAAAATTTCGGAGCATTGTGCATGCTGTTCAAGCTGGAATTTTTGTGGAAAG AATGTACCGAAAAACATATCATATGGTTGGTTTGGCATATCCAGCAGCTGTCATCGTAACATTAAAGGAT GTTGATAAATGGTCTTTCGATGTATTTGCCCTAAATGAAGCAAGTGGAGAGCATAGTCTGAAGTTTATGA TTTATGAACTGTTTACCAGATATGATCTTATCAACCGTTTCAAGATTCCTGTTTCTTGCCTAATCACCTT TGCAGAAGCTTTAGAAGTTGGTTACAGCAAGTACAAAAATCCATATCACAATTTGATTCATGCAGCTGAT GTCACTCAAACTGTGCATTACATAATGCTTCATACAGGTATCATGCACTGGCTCACTGAACTGGAAATTT TAGCAATGGTCTTTGCTGCTGCCATTCATGATTATGAGCATACAGGGACAACAAACAACTTTCACATTCA GACAAGGTCAGATGTTGCCATTTTGTATAATGATCGCTCTGTCCTTGAGAATCACCACGTGAGTGCAGCT TATCGACTTATGCAAGAAGAAGAAATGAATATCTTGATAAATTTATCCAAAGATGACTGGAGGGATCTTC GGAACCTAGTGATTGAAATGGTTTTATCTACAGACATGTCAGGTCACTTCCAGCAAATTAAAAATATAAG AAACAGTTTGCAGCAGCCTGAAGGGATTGACAGAGCCAAAACCATGTCCCTGATTCTCCACGCAGCAGAC ATCAGCCACCCAGCCAAATCCTGGAAGCTGCATTATCGGTGGACCATGGCCCTAATGGAGGAGTTTTTCC TGCAGGGAGATAAAGAAGCTGAATTAGGGCTTCCATTTTCCCCACTTTGTGATCGGAAGTCAACCATGGT GGCCCAGTCACAAATAGGTTTCATCGATTTCATAGTAGAGCCAACATTTTCTCTTCTGACAGACTCAACA GAGAAAATTGTTATTCCTCTTATAGAGGAAGCCTCAAAAGCCGAAACTTCTTCCTATGTGGCAAGCAGCT CAACCACCATTGTGGGGTTACACATTGCTGATGCACTAAGACGATCAAATACAAAAGGCTCCATGAGTGA TGGGTCCTATTCCCCAGACTACTCCCTTGCAGCAGTGGACCTGAAGAGTTTCAAGAACAACCTGGTGGAC ATCATTCAGCAGAACAAAGAGAGGTGGAAAGAGTTAGCTGCACAAGAAGCAAGAACCAGTTCACAGAAGT GTGAGTTTATTCATCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234336 representing NM_001258313
Red=Cloning site Green=Tags(s) MDDHVTIRKKHLQRPIFRLRCLVKQLERGDVNVVDLKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQT DSVPSEVRDWLASTFTRKMGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKTYHMVGLAYPAAVIVTLKD VDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAAD VTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAA YRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAAD ISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDST EKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVD IIQQNKERWKELAAQEARTSSQKCEFIHQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001258313 |
ORF Size | 1557 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001258313.1, NP_001245242.1 |
RefSeq Size | 2338 bp |
RefSeq ORF | 1560 bp |
Locus ID | 5136 |
Cytogenetics | 2q32.1 |
Protein Families | Druggable Genome |
Protein Pathways | Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction |
MW | 60 kDa |
Gene Summary | 'Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]' |
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