FASTK (NM_001258461) Human Tagged ORF Clone

CAT#: RC234350

FASTK (Myc-DDK tagged) - Homo sapiens Fas-activated serine/threonine kinase (FASTK), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FASTK
• Synonyms: FAST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234350 representing NM_001258461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGGCCGCGGGGGGAACCCGGCCCCCGGGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGC
  CCGGGGAGTCATGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGC
  TCGGCTGTCTGGCCTGCTGCTGATCCCTCCAGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGAC
  CGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAACAGGCGAAGA
  GCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGC
  GCTTCGTCGTCTGGGCCAGCTCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAG
  GACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACACCATCCACGTGTGTCTGCACC
  TTGCAGTCTTACTTGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCG
  CCTCCCTCCGAAGCCACCTCCCCCTTTGCAGCCCCTTCTCCGAGAGGCAAGGCCAGAGGAACTGACTCCC
  CACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCTGGAAGCCA
  TTGCCCACTTCCTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTT
  TGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGCCCTGCCTTGAGAGGATCCTGGCTCGGGAA
  GCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCA
  GAGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCTCTGATTGT
  GCGTCGCTACCTCTCCCTGCTGGACACGGCCGTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTT
  CCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCAAGTACAGTC
  ACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGT
  GCCTCCAGGCTACTGCACAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACC
  CAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAGCGCCACTACTC
  GAGACCCTGCCCAGAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCT
  GCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGCCGCTACCCTTC
  GAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCTCCAGGCCCTGG
  GCCTGCGCTGGGGGCCTGAAGGGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234350 representing NM_001258461
  Red=Cloning site Green=Tags(s)
  
  MRRPRGEPGPRAPRPTEGATCAGPGESWSPSPNSMLRVLLSAQTSPARLSGLLLIPPVQPCCLGPSKWGD
  RPVGGGPSAGPVQGLQRLLEQAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQLLGSRPRPPPVEQVTLQ
  DLSQLIIRNCPSFDIHTIHVCLHLAVLLGFPSDGPLVCALEQERRLRLPPKPPPPLQPLLREARPEELTP
  HVMVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCLERILARE
  AGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSLLDTAVELELPGYRGPRL
  PRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTDFLLCASSSGAVLPVRT
  QDPFLPYPPRSCPQGQAASSATTRDPAQRVVLVLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPF
  EELESQRGLPQLKSYLRQKLQALGLRWGPEGG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258461
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258461.1, NP_001245390.1
• RefSeq Size: 1790
• RefSeq ORF: 1569
• Locus ID: 10922
• Protein Families: Druggable Genome, Protein Kinase
• MW: 58.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq, Jul 2008]