ITPRIP (NM_001272013) Human Tagged ORF Clone

CAT#: RC234407

ITPRIP (Myc-DDK tagged) - Homo sapiens inositol 1,4,5-trisphosphate receptor interacting protein (ITPRIP), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITPRIP
• Synonyms: bA127L20; bA127L20.2; DANGER; KIAA1754
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234407 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGGGCTCTTCCGCGTGTGTCTGGTGGTGGTGACGGCCATCATCAACCACCCGCTGCTGTTCC
  CGCGGGAGAACGCCACAGTCCCCGAGAACGAGGAGGAGATCATCCGCAAGATGCAGGCGCACCAGGAGAA
  GCTGCAGCTGGAGCAGTTGCGCCTGGAGGAGGAGGTGGCTCGGCTGGCGGCCGAAAAGGAGGCACTGGAG
  CAGGTGGCGGAGGAGGGCAGGCAGCAGAACGAGACACGCGTGGCCTGGGACCTCTGGAGCACCCTCTGCA
  TGATCCTCTTCCTGATGATCGAGGTGTGGCGGCAGGACCACCAGGAGGGGCCCTCACCTGAGTGCCTGGG
  CGGTGAGGAGGATGAGCTGCCTGGGCTGGGGGGCGCCCCCTTGCAGGGCCTCACCCTGCCCAACAAGGCC
  ACGCTTGGCCACTTTTATGAGCGCTGCATCCGGGGGGCCACGGCCGATGCAGCCCGTACCCGGGAGTTCC
  TGGAAGGCTTCGTGGATGACTTGCTGGAAGCCCTGAGGAGCCTCTGCAACCGGGACACCGACATGGAGGT
  GGAGGACTTCATTGGCGTGGACAGCATGTACGAGAACTGGCAGGTGGACAGGCCACTGCTGTGCCACCTT
  TTCGTGCCCTTCACACCCCCCGAGCCCTACCGCTTCCACCCAGAGCTCTGGTGCTCCGGCCGCTCAGTGC
  CCCTGGATCGCCAGGGCTACGGCCAGATCAAGGTGGTCCGCGCCGATGGGGACACATTGAGCTGCATCTG
  CGGCAAGACCAAGCTCGGGGAAGACATGCTGTGTCTCCTGCACGGCAGGAACAGCATGGCGCCTCCCTGC
  GGCGACATGGAGAACCTGCTGTGTGCCACAGATTCCCTGTACCTGGACACGATGCAGGTCATGAAGTGGT
  TCCAGACGGCCCTCACCAGAGCCTGGAAGGGCATCGCCCACAAGTACGAGTTCGACCTGGCCTTTGGCCA
  GCTGGACAGCCCGGGGTCCCTGAAGATCAAGTTCCGTTCAGGGAAGTTCATGCCCTTCAACCTGATTCCT
  GTGATCCAGTGTGATGACTCGGACCTGTACTTTGTCTCCCACCTTCCCAGGGAGCCCTCTGAGGGCACCC
  CAGCCTCCAGCACAGACTGGCTCCTGTCCTTTGCTGTCTATGAGCGACACTTCCTCAGGACGACACTAAA
  GGCACTGCCCGAGGGCGCCTGCCACCTCAGCTGCCTGCAGATAGCATCCTTCCTGCTCTCCAAGCAGAGC
  CGCCTGACCGGTCCCAGCGGGCTCAGCAGCTACCACCTGAAGACGGCCCTACTGCACCTCCTACTCCTCC
  GGCAGGCCGCCGACTGGAAGGCGGGGCAGCTGGACGCTCGTCTGCACGAGTTGCTGTGCTTCCTGGAGAA
  GAGCTTGCTCCAGAAGAAGCTCCACCACTTCTTCATCGGCAACCGCAAGGTGCCTGAGGCCATGGGACTC
  CCTGAGGCCGTGCTCAGGGCCGAGCCCCTCAACCTCTTCCGGCCCTTCGTCCTGCAGCGAAGCCTTTACC
  GTAAGACACTGGACTCCTTCTATGAGATGCTCAAGAATGCCCCAGCGCTCATTAGCGAGTATTCCCTACA
  TGTCCCCTCAGACCAGCCTACCCCAAAAAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234407 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMGLFRVCLVVVTAIINHPLLFPRENATVPENEEEIIRKMQAHQEKLQLEQLRLEEEVARLAAEKEALE
  QVAEEGRQQNETRVAWDLWSTLCMILFLMIEVWRQDHQEGPSPECLGGEEDELPGLGGAPLQGLTLPNKA
  TLGHFYERCIRGATADAARTREFLEGFVDDLLEALRSLCNRDTDMEVEDFIGVDSMYENWQVDRPLLCHL
  FVPFTPPEPYRFHPELWCSGRSVPLDRQGYGQIKVVRADGDTLSCICGKTKLGEDMLCLLHGRNSMAPPC
  GDMENLLCATDSLYLDTMQVMKWFQTALTRAWKGIAHKYEFDLAFGQLDSPGSLKIKFRSGKFMPFNLIP
  VIQCDDSDLYFVSHLPREPSEGTPASSTDWLLSFAVYERHFLRTTLKALPEGACHLSCLQIASFLLSKQS
  RLTGPSGLSSYHLKTALLHLLLLRQAADWKAGQLDARLHELLCFLEKSLLQKKLHHFFIGNRKVPEAMGL
  PEAVLRAEPLNLFRPFVLQRSLYRKTLDSFYEMLKNAPALISEYSLHVPSDQPTPKS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001272013
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272013.1, NP_001258942.1
• RefSeq Size: 6633 bp
• RefSeq ORF: 1644 bp
• Locus ID: 85450
• MW: 62.1 kDa
• Gene Summary: This gene encodes a membrane-associated protein that binds the inositol 1,4,5-trisphosphate receptor (ITPR). The encoded protein enhances the sensitivity of ITPR to intracellular calcium signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]