ZNF195 (NM_001242843) Human Tagged ORF Clone

CAT#: RC234436

ZNF195 (Myc-DDK tagged) - Homo sapiens zinc finger protein 195 (ZNF195), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF195
• Synonyms: HRF1; ZNFP104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234436 representing NM_001242843
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGTGCTCAGACTCTGTTGACGTTCAGGGATGTGGCCATAGAATTCTCCCTGGAGGAGTGGAAAT
  GCCTGGACCTCGCTCAGCAGAATTTGTACAGGGATGTGATGTTGGAGAACTACAGAAACTTGTTCTCCGT
  TGGTCTCACTGTCTGTAAGCCAGGCCTGATCACCTGCCTGGAGCAACGAAAAGAGCCCTGGAATGTGAAG
  AGACAGGAGGCAGCAGACGGACATCCAGCTATGTCTTCTCATTTTACCCAAGACCTTCTGCCAGAGCAGG
  GCATACAAGATGCATTCCCAAAAAGAATACTGAGAGGATATGGAAATTGTGGCCTTGATAATTTATATTT
  AAGGAAAGACTGGGAAAGTTTAGATGAGTGTAAGTTGCAAAAAGATTATAATGGACTTAACCAATGTTCA
  TCAACTACCCATAGCAAAATCTTTCAATATAATAAATATGTTAAAATCTTTGATAACTTTTCAAATTTAC
  ATAGACGTAATATAAGTAATACTGGAGAGAAACCTTTCAAATGTCAAGAATGTGGCAAATCCTTTCAAAT
  GCTCTCATTCCTAACTGAACATCAGAAAATTCACACTGGAAAAAAATTCCAAAAATGTGGAGAATGTGGC
  AAAACCTTTATCCAGTGCTCACACTTTACTGAACCTGAGAACATTGACACTGGAGAGAAACCTTACAAGT
  GTCAAGAATGTAACAACGTCATTAAAACTTGCTCAGTCCTTACTAAAAATAGAATTTATGCCGGAGGGGA
  ACATTACAGATGTGAAGAATTTGGCAAAGTATTTAACCAGTGCTCCCACCTTACTGAACATGAGCATGGT
  ACTGAGGAAAAACCCTGCAAATATGAAGAGTGCAGCAGTGTCTTTATCTCTTGCTCAAGCCTTTCTAATC
  AACAGATGATTCTTGCTGGAGAGAAGCTCTCCAAATGTGAAACATGGTACAAAGGTTTTAACCACAGCCC
  AAATCCTTCCAAACACCAGAGAAATGAGATTGGAGGGAAACCTTTCAAATGTGAGGAATGTGACAGCATC
  TTCAAGTGGTTCTCAGACCTTACTAAACATAAGAGAATTCACACTGGTGAGAAACCATACAAATGTGACG
  AATGTGGGAAAGCCTATACACAGTCCTCACACCTCAGTGAACACAGGAGGATTCACACCGGAGAGAAACC
  CTACCAATGTGAAGAATGTGGGAAGGTCTTCAGAACTTGCTCAAGCCTTTCTAACCATAAGAGAACTCAT
  TCTGAAGAAAAACCCTACACGTGTGAAGAATGTGGCAACATCTTTAAGCAGTTATCAGACCTCACTAAGC
  ATAAGAAAACCCATACTGGAGAGAAGCCCTACAAATGTGACGAATGTGGAAAAAACTTTACCCAGTCCTC
  CAACCTTATTGTACATAAGAGAATTCATACTGGAGAGAAACCCTACAAGTGTGAAGAATGTGGCAGAGTC
  TTCATGTGGTTCTCAGACATTACCAAACATAAGAAAACCCATACTGGAGAGAAACCCTACAAATGTGACG
  AATGTGGAAAAAACTTTACCCAGTCCTCAAACCTTATTGTACATAAGAGAATTCATACTGGAGAGAAACC
  CTACAAGTGTGAAAAGTGTGGCAAAGCCTTCACCCAGTTCTCACACCTGACTGTACATGAAAGCATTCAT
  ACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234436 representing NM_001242843
  Red=Cloning site Green=Tags(s)
  
  MAGAQTLLTFRDVAIEFSLEEWKCLDLAQQNLYRDVMLENYRNLFSVGLTVCKPGLITCLEQRKEPWNVK
  RQEAADGHPAMSSHFTQDLLPEQGIQDAFPKRILRGYGNCGLDNLYLRKDWESLDECKLQKDYNGLNQCS
  STTHSKIFQYNKYVKIFDNFSNLHRRNISNTGEKPFKCQECGKSFQMLSFLTEHQKIHTGKKFQKCGECG
  KTFIQCSHFTEPENIDTGEKPYKCQECNNVIKTCSVLTKNRIYAGGEHYRCEEFGKVFNQCSHLTEHEHG
  TEEKPCKYEECSSVFISCSSLSNQQMILAGEKLSKCETWYKGFNHSPNPSKHQRNEIGGKPFKCEECDSI
  FKWFSDLTKHKRIHTGEKPYKCDECGKAYTQSSHLSEHRRIHTGEKPYQCEECGKVFRTCSSLSNHKRTH
  SEEKPYTCEECGNIFKQLSDLTKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEECGRV
  FMWFSDITKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEKCGKAFTQFSHLTVHESIH
  T
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242843
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242843.1, NP_001229772.1
• RefSeq Size: 3146 bp
• RefSeq ORF: 1686 bp
• Locus ID: 7748
• Cytogenetics: 11p15.4
• Protein Families: Transcription Factors
• MW: 65.4 kDa
• Gene Summary: 'This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]'