USP39 (NM_001256725) Human Tagged ORF Clone

CAT#: RC234447

USP39 (Myc-DDK tagged) - Homo sapiens ubiquitin specific peptidase 39 (USP39), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP39
• Synonyms: 65K; CGI-21; HSPC332; SAD1; SNRNP65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234447 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGCCGGTCTAAGCGGGAGTCTCGCGGTTCCACTCGCGGGAAGCGAGAGTCTGAGTCGCGGGGCA
  GCTCCGGTCGCGTCAAGCGGGAGCGAGATCGGGAGCGGGAGCCTGAGGCGGCGAGCTCCCGGGGCAGCCC
  TGTGCGCGTGAAGCGGGAGTTCGAGCCGGCGAGCGCGCGCGAGGCCCCGGCTTCTGTTGTCCCGTTTGTG
  CGGGTGAAGCGGGAGCGCGAGGTCGATGAGGACTCGGAGCCTGAGCGGGAGGTGCGAGCAAAGAATGGCC
  GAGTGGATTCTGAGGACCGGAGGAGCCGCCACTGCCCGTACCTGGACACCATTAACAGGAGTGTGCTGGA
  CTTTGACTTTGAGAAACTGTGTTCTATCTCCCTCTCACACATCAATGCTTATGCCTGTCTGGTGTGTGGC
  AAGTACTTTCAAGGCCGGGGTTTGAAGTCTCACGCCTACATTCACAGTGTCCAGTTTAGCCACCATGTTT
  TCCTCAACCTCCACACCCTCAAGTTTTACTGCCTTCCAGACAACTATGAGATCATCGATTCCTCATTGGA
  GGATATCACGTATGTGTTGAAGCCCACTTTCACAAAGCAGCAAATTGCAAACTTGGACAAGCAAGCCAAA
  TTGTCCCGGGCATATGATGGTACCACTTACCTGCCGGGTATTGTGGGACTGAATAACATAAAGGCCAATG
  ATTATGCCAACGCTGTCCTTCAGGCTCTATCTAATGTTCCTCCTCTCCGGAACTACTTTCTGGAAGAAGA
  CAATTATAAGAACATCAAACGTCCTCCAGGGGATATCATGTTCTTGTTGGTCCAGCGTTTTGGAGAGCTG
  ATGAGAAAGCTCTGGAACCCTCGAAATTTCAAGGCACATGTGTCTCCCCATGAGATGCTTCAGGCAGTTG
  TACTTTGCAGTAAGAAGACTTTTCAGATCACCAAACAAGGAGATGGCGTTGACTTTCTGTCTTGGTTTCT
  GAATGCTCTGCACTCAGCTCTGGGGGGCACAAAGAAGAAAAAGAAGACTATTGTGACTGATGTTTTCCAG
  GGGTCCATGAGGATCTTCACTAAAAAGCTTCCCCATCCTGATCTGCCAGCAGAAGAAAAAGAGCAGTTGC
  TCCATAATGACGAGTACCAGGAGACAATGGTGGAGTCCACTTTTATGTACCTGACGCTGGACCTTCCTAC
  TGCCCCCCTCTACAAGGACGAGAAGGAGCAGCTCATCATTCCCCAAGTGCCACTCTTCAACATCCTGGCT
  AAGTTCAATGGCATCACTGAGAAGGAATATAAGACTTACAAGGAGAACTTTCTGAAGCGCTTCCAGCTTA
  CCAAGTTGCCTCCATATCTAATCTTTTGTATCAAGAGATTCACTAAGAACAACTTCTTTGTTGAGAAGAA
  TCCAACTATTGTCAATTTCCCTATTACAAATGTGGATCTGAGAGAATACTTGTCTGAAGAAGTACAAGCA
  GTACACAAGAATACCACCTATGACCTCATTGCCAACATCGTGCATGACGGCAAGCCCTCCGAGGGCTCCT
  ACCGGATCCACGTGCTTCATCATGGGACAGGCAAATGGTATGAATTACAAGACCTCCAGGTGACTGACAT
  CCTTCCCCAGATGATCACACTGTCAGAGGCTTACATTCAGATTTGGAAGAGGCGAGATAATGATGAAACC
  AACCAGCAGGGGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234447 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGRSKRESRGSTRGKRESESRGSSGRVKRERDREREPEAASSRGSPVRVKREFEPASAREAPASVVPFV
  RVKREREVDEDSEPEREVRAKNGRVDSEDRRSRHCPYLDTINRSVLDFDFEKLCSISLSHINAYACLVCG
  KYFQGRGLKSHAYIHSVQFSHHVFLNLHTLKFYCLPDNYEIIDSSLEDITYVLKPTFTKQQIANLDKQAK
  LSRAYDGTTYLPGIVGLNNIKANDYANAVLQALSNVPPLRNYFLEEDNYKNIKRPPGDIMFLLVQRFGEL
  MRKLWNPRNFKAHVSPHEMLQAVVLCSKKTFQITKQGDGVDFLSWFLNALHSALGGTKKKKKTIVTDVFQ
  GSMRIFTKKLPHPDLPAEEKEQLLHNDEYQETMVESTFMYLTLDLPTAPLYKDEKEQLIIPQVPLFNILA
  KFNGITEKEYKTYKENFLKRFQLTKLPPYLIFCIKRFTKNNFFVEKNPTIVNFPITNVDLREYLSEEVQA
  VHKNTTYDLIANIVHDGKPSEGSYRIHVLHHGTGKWYELQDLQVTDILPQMITLSEAYIQIWKRRDNDET
  NQQGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256725
• ORF Size: 1695 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256725.1, NP_001243654.1
• RefSeq Size: 2135 bp
• RefSeq ORF: 1698 bp
• Locus ID: 10713
• Protein Families: Protease
• Protein Pathways: Spliceosome
• MW: 65.4 kDa