SMARCAD1 (NM_001254949) Human Tagged ORF Clone

CAT#: RC234518

SMARCAD1 (Myc-DDK tagged) - Homo sapiens SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 (SMARCAD1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMARCAD1
• Synonyms: ADERM; BASNS; ETL1; HEL1; HRZ
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234518 representing NM_001254949
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAAACTAATGGCTTATCAGAAGATTTGATATGGCACTGTAAAACACTGATCCAAGAAAGAGATG
  TAGTTATAAGGCTTATGAACAAATGTGAAGACATTTCAAATAAATTGACCAAACAAGTTACCATGCTTAC
  TGGAAATGGAGGTGGATGGAACATAGAACAACCTTCCATTCTAAACCAAAGTTTGTCACTCAAGCCCTAT
  CAGAAGGTTGGTTTGAATTGGCTGGCATTGGTACATAAACATGGACTTAATGGCATTTTGGCAGATGAAA
  TGGGCCTAGGAAAAACTATTCAAGCCATTGCATTTCTGGCATACCTCTATCAGGAGGGTAATAATGGTCC
  TCATTTGATCGTTGTTCCAGCTTCAACTATAGATAACTGGTTAAGGGAAGTTAATTTATGGTGCCCTACT
  TTGAAGGTCCTCTGTTACTATGGTTCTCAAGAAGAACGTAAACAAATTAGATTTAACATTCATAGTAGAT
  ATGAAGATTACAATGTAATTGTGACCACATATAACTGTGCGATCAGCAGTTCTGATGACCGTAGTCTGTT
  TCGACGGCTGAAACTTAATTACGCAATTTTTGATGAGGGCCATATGCTGAAGAATATGGGCTCCATTCGC
  TACCAGCACCTTATGACAATTAATGCAAATAACCGTTTGCTGCTCACAGGCACACCTGTACAGAACAATC
  TGTTAGAACTCATGTCGCTGTTGAATTTTGTTATGCCACACATGTTTAGTAGTAGCACCAGTGAAATACG
  AAGAATGTTTTCCTCTAAGACAAAATCAGCAGATGAGCAAAGCATATATGAAAAGGAGAGAATAGCACAT
  GCAAAACAAATTATAAAGCCATTTATTCTCAGAAGAGTAAAAGAAGAGGTTCTCAAGCAGTTACCCCCCA
  AGAAAGATCGAATTGAGTTGTGTGCAATGTCGGAGAAGCAGGAGCAACTCTATTTGGGTCTTTTCAACAG
  ATTGAAAAAATCTATCAATAACTTGGAAAAAAACACAGAAATGTGCAATGTCATGATGCAGTTGAGGAAA
  ATGGCCAATCATCCTTTATTACATCGCCAATATTACACAGCTGAAAAACTCAAGGAAATGTCTCAGCTTA
  TGCTAAAGGAACCTACACATTGTGAGGCTAACCCTGACCTGATCTTTGAAGATATGGAAGTTATGACAGA
  CTTCGAACTACATGTACTTTGTAAACAGTACCGACACATTAATAACTTTCAGTTAGACATGGACTTGATT
  TTAGATTCTGGAAAATTTCGAGTTTTAGGATGCATCTTGTCTGAATTGAAACAGAAGGGTGATAGAGTTG
  TGTTATTTAGCCAATTTACCATGATGCTGGATATCTTAGAGGTTCTATTAAAACATCATCAGCATAGGTA
  CCTCAGATTAGATGGAAAGACTCAGATTTCTGAAAGGATTCATCTAATTGATGAGTTTAATACCGATATG
  GATATCTTTGTGTTTCTGCTATCAACAAAAGCTGGTGGATTAGGAATAAATCTGACTTCAGCAAATGTTG
  TTATACTTCACGATATTGACTGTAATCCTTATAATGACAAACAAGCAGAAGATAGATGCCATAGAGTAGG
  CCAGACTAAAGAAGTACTAGTTATAAAACTAATAAGCCAAGGGACGATTGAAGAATCCATGCTAAAAATT
  AACCAACAGAAATTGAAACTAGAACAGGATATGACTACAGTAGATGAAGGTGATGAAGGGAGTATGCCAG
  CAGATATAGCCACATTACTAAAAACATCAATGGGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234518 representing NM_001254949
  Red=Cloning site Green=Tags(s)
  
  MSKTNGLSEDLIWHCKTLIQERDVVIRLMNKCEDISNKLTKQVTMLTGNGGGWNIEQPSILNQSLSLKPY
  QKVGLNWLALVHKHGLNGILADEMGLGKTIQAIAFLAYLYQEGNNGPHLIVVPASTIDNWLREVNLWCPT
  LKVLCYYGSQEERKQIRFNIHSRYEDYNVIVTTYNCAISSSDDRSLFRRLKLNYAIFDEGHMLKNMGSIR
  YQHLMTINANNRLLLTGTPVQNNLLELMSLLNFVMPHMFSSSTSEIRRMFSSKTKSADEQSIYEKERIAH
  AKQIIKPFILRRVKEEVLKQLPPKKDRIELCAMSEKQEQLYLGLFNRLKKSINNLEKNTEMCNVMMQLRK
  MANHPLLHRQYYTAEKLKEMSQLMLKEPTHCEANPDLIFEDMEVMTDFELHVLCKQYRHINNFQLDMDLI
  LDSGKFRVLGCILSELKQKGDRVVLFSQFTMMLDILEVLLKHHQHRYLRLDGKTQISERIHLIDEFNTDM
  DIFVFLLSTKAGGLGINLTSANVVILHDIDCNPYNDKQAEDRCHRVGQTKEVLVIKLISQGTIEESMLKI
  NQQKLKLEQDMTTVDEGDEGSMPADIATLLKTSMGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001254949
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001254949.1, NP_001241878.1
• RefSeq Size: 3950
• RefSeq ORF: 1791
• Locus ID: 56916
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• MW: 69.2 kDa
• Gene Summary: This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]