Paxillin (PXN) (NM_001243756) Human Tagged ORF Clone

CAT#: RC234550

PXN (Myc-DDK tagged) - Homo sapiens paxillin (PXN), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PXN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234550 representing NM_001243756
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACCTCGACGCCCTGCTGGCGGACTTGGAGTCTACCACCTCCCACATCTCCAAACGGCCTGTGT
  TCTTGTCGGAGGAGACCCCCTACTCATACCCAACTGGAAACCACACATACCAGGAGATTGCCGTGCCACC
  CCCCGTCCCCCCACCCCCGTCCAGCGAGGCCCTCAATGGCACAATCCTTGACCCCTTAGACCAGTGGCAG
  CCCAGCAGCTCCCGATTCATCCACCAGCAGCCTCAGTCCTCATCACCTGTGTACGGCTCCAGTGCCAAAA
  CTTCCAGTGTCTCCAACCCTCAGGACAGTGTTGGCTCTCCGTGCTCCCGAGTGGGTGAGGAGGAGCACGT
  CTACAGCTTCCCCAACAAGCAGAAATCAGCTGAGCCTTCACCCACCGTAATGAGCACGTCCCTGGGCAGC
  AACCTTTCTGAACTCGACCGCCTGCTGCTGGAACTGAACGCTGTACAGCATAACCCGCCAGGCTTCCCTG
  CAGATGAGGCCAACTCAAGCCCCCCGCTTCCTGGGGCCCTGAGCCCCCTCTATGGTGTCCCAGAGACTAA
  CAGCCCCTTGGGAGGCAAAGCTGGGCCCCTGACGAAAGAGAAGCCTAAGCGGAATGGGGGCCGGGGCCTG
  GAGGACGTGCGGCCCAGTGTGGAGAGTCTCTTGGATGAACTGGAGAGCTCCGTGCCCAGCCCCGTCCCTG
  CCATCACTGTGAACCAGGGCGAGATGAGCAGCCCGCAGCGCGTCACCTCCACCCAACAGCAGACACGCAT
  CTCGGCCTCCTCTGCCACCAGGGAGCTGGACGAGCTGATGGCTTCGCTGTCGGATTTCAAGGGCTCCTGG
  CCCCTGGAGGAGGTTGTACTTCTTGTCTCCATCAGCTCATCTGTCCAGGAGGGAGAAAAGTACCCCCATC
  CCTGTGCTGCCAGACACCGTACCCCGAGCCTCAGGAGTCCTGACCAGCCCCCTCCGTGTCCACAGTTCAT
  GGCCCAGGGGAAGACAGGGAGCAGCTCACCCCCTGGGGGGCCCCCGAAGCCCGGGAGCCAGCTGGACAGC
  ATGCTGGGGAGCCTGCAGTCTGACCTGAACAAGCTGGGGGTCGCCACAGTCGCCAAAGGAGTCTGCGGGG
  CCTGCAAGAAGCCCATCGCCGGGCAGGTTGTGACCGCCATGGGGAAGACGTGGCACCCCGAGCACTTCGT
  CTGCACCCACTGCCAGGAGGAGATCGGATCCCGGAACTTCTTCGAGCGGGATGGACAGCCCTACTGTGAA
  AAGGACTACCACAACCTCTTCTCCCCGCGCTGCTACTACTGCAACGGCCCCATCCTGGATAAAGTGGTGA
  CAGCCCTTGACCGGACGTGGCACCCTGAACACTTCTTCTGTGCACAGTGTGGAGCCTTCTTTGGTCCCGA
  AGGGTTCCACGAGAAGGACGGCAAGGCCTACTGTCGCAAGGACTACTTCGACATGTTCGCACCCAAGTGT
  GGCGGCTGCGCCCGGGCCATCCTGGAGAACTATATCTCAGCCCTCAACACGCTGTGGCATCCTGAGTGCT
  TTGTGTGCCGGGAATGCTTCACGCCATTCGTGAACGGCAGCTTCTTCGAGCACGACGGGCAGCCCTACTG
  TGAGGTGCACTACCACGAGCGGCGCGGCTCGCTGTGTTCTGGCTGCCAGAAGCCCATCACCGGCCGCTGC
  ATCACCGCCATGGCCAAGAAGTTCCACCCCGAGCACTTCGTCTGTGCCTTCTGCCTCAAGCAGCTCAACA
  AGGGCACCTTCAAGGAGCAGAACGACAAGCCTTACTGTCAGAACTGCTTCCTCAAGCTCTTCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234550 representing NM_001243756
  Red=Cloning site Green=Tags(s)
  
  MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQ
  PSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGS
  NLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGL
  EDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKGSW
  PLEEVVLLVSISSSVQEGEKYPHPCAARHRTPSLRSPDQPPPCPQFMAQGKTGSSSPPGGPPKPGSQLDS
  MLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCE
  KDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKC
  GGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRC
  ITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243756
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243756.1, NP_001230685.1
• RefSeq Size: 3835 bp
• RefSeq ORF: 1818 bp
• Locus ID: 5829
• Cytogenetics: 12q24.23
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway
• MW: 66.7 kDa
• Gene Summary: 'This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]'