SPHK2 (NM_001204160) Human Tagged ORF Clone

CAT#: RC234574

SPHK2 (Myc-DDK tagged) - Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPHK2
• Synonyms: SK-2; SK 2; SPK-2; SPK 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234574 representing NM_001204160
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGCCCCCACCGCCACTGGCTGCCAGCACCCCGCTCCTCCATGGCGAGTTTGGCTCCTACCCAG
  CCCGAGGCCCACGCTTTGCCCTCACCCTTACATCGCAGGCCCTGCACATACAGCGGCTGCGCCCCAAACC
  TGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGTCTCAGGCTGCTGCACCCTGCGAAGCCGC
  AGCCCCTCAGACTCAGCGGCCTACTTCTGCATCTACACCTACCCTCGGGGCCGGCGCGGGGCCCGGCGCA
  GAGCCACTCGCACCTTCCGGGCAGATGGGGCCGCCACCTACGAAGAGAACCGTGCCGAGGCCCAGCGCTG
  GGCCACTGCCCTCACCTGTCTGCTCCGAGGACTGCCACTGCCCGGGGATGGGGAGATCACCCCTGACCTG
  CTACCTCGGCCGCCCCGGTTGCTTCTATTGGTCAATCCCTTTGGGGGTCGGGGCCTGGCCTGGCAGTGGT
  GTAAGAACCACGTGCTTCCCATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATCCAGACAGAACGACA
  GAACCACGCCCGGGAGCTGGTCCAGGGGCTGAGCCTGAGTGAGTGGGATGGCATCGTCACGGTCTCGGGA
  GACGGGCTGCTCCATGAGGTGCTGAACGGGCTCCTAGATCGCCCTGACTGGGAGGAAGCTGTGAAGATGC
  CTGTGGGCATCCTCCCCTGCGGCTCGGGCAACGCGCTGGCCGGAGCAGTGAACCAGCACGGGGGATTTGA
  GCCAGCCCTGGGCCTCGACCTGTTGCTCAACTGCTCACTGTTGCTGTGCCGGGGTGGTGGCCACCCACTG
  GACCTGCTCTCCGTGACGCTGGCCTCGGGCTCCCGCTGTTTCTCCTTCCTGTCTGTGGCCTGGGGCTTCG
  TGTCAGATGTGGATATCCAGAGCGAGCGCTTCAGGGCCTTGGGCAGTGCCCGCTTCACACTGGGCACGGT
  GCTGGGCCTCGCCACACTGCACACCTACCGCGGACGCCTCTCCTACCTCCCCGCCACTGTGGAACCTGCC
  TCGCCCACCCCTGCCCATAGCCTGCCTCGTGCCAAGTCGGAGCTGACCCTAACCCCAGACCCAGCCCCGC
  CCATGGCCCACTCACCCCTGCATCGTTCTGTGTCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCCTGGC
  CTCTCCTGGCTCGCCAGAACCCCTGCCCATCCTGTCCCTCAACGGTGGGGGCCCAGAGCTGGCTGGGGAC
  TGGGGTGGGGCTGGGGATGCTCCGCTGTCCCCGGACCCACTGCTGTCTTCACCTCCTGGCTCTCCCAAGG
  CAGCTCTACACTCACCCGTCTCCGAAGGGGCCCCCGTAATTCCCCCATCCTCTGGGCTCCCACTTCCCAC
  CCCTGATGCCCGGGTAGGGGCCTCCACCTGCGGCCCGCCCGACCACCTGCTGCCTCCGCTGGGCACCCCG
  CTGCCCCCAGACTGGGTGACGCTGGAGGGGGACTTTGTGCTCATGTTGGCCATCTCGCCCAGCCACCTAG
  GCGCTGACCTGGTGGCAGCTCCGCATGCGCGCTTCGACGACGGCCTGGTGCACCTGTGCTGGGTGCGTAG
  CGGCATCTCGCGGGCTGCGCTGCTGCGCCTTTTCTTGGCCATGGAGCGTGGTAGCCACTTCAGCCTGGGC
  TGTCCGCAGCTGGGCTACGCCGCGGCCCGTGCCTTCCGCCTAGAGCCGCTCACACCACGCGGCGTGCTCA
  CAGTGGACGGGGAGCAGGTGGAGTATGGGCCGCTACAGGCACAGATGCACCCTGGCATCGGTACACTGCT
  CACTGGGCCTCCTGGCTGCCCGGGGCGGGAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234574 representing NM_001204160
  Red=Cloning site Green=Tags(s)
  
  MAPPPPPLAASTPLLHGEFGSYPARGPRFALTLTSQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRSR
  SPSDSAAYFCIYTYPRGRRGARRRATRTFRADGAATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDL
  LPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSG
  DGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPL
  DLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPA
  SPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGD
  WGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTP
  LPPDWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLG
  CPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204160
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001204160.1, NM_001204160.2, NP_001191089.1
• RefSeq Size: 2684
• RefSeq ORF: 1857
• Locus ID: 56848
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
• MW: 65.7 kDa
• Gene Summary: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]