DAXX (NM_001254717) Human Tagged ORF Clone

CAT#: RC234672

DAXX (Myc-DDK tagged) - Homo sapiens death-domain associated protein (DAXX), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAXX
• Synonyms: BING2; DAP6; EAP1; SMIM40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234672 representing NM_001254717
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACAGCAGACCACCCTGAGGTGGTCCCATTCCTCTATAACCGGCAGCAACGTGCCCACTCTCTGT
  TTTTGGCCTCGGCGGAGTTCTGCAACATCCTCTCTAGGGTCCTGTCTCGGGCCCGGAGCCGGCCAGCCAA
  GCTCTATGTCTACATCAATGAGCTCTGCACTGTTCTCAAGGCCCACTCAGCCAAAAAGAAGCTGAACTTG
  GCCCCTGCCGCCACCACCTCCAATGAGCCCTCTGGGAATAACCCTCCCACACACCTCTCCTTGGACCCCA
  CAAATGCTGAAAACACTGCCTCTCAGTCTCCAAGGACCCGTGGTTCCCGGCGGCAGATCCAGCGTTTGGA
  GCAGCTGCTGGCGCTCTATGTGGCAGAGATCCGGCGGCTGCAGGAAAAGGAGTTGGATCTCTCAGAATTG
  GATGACCCAGACTCCGCATACCTGCAGGAGGCACGGTTGAAGCGTAAGCTGATCCGCCTCTTTGGGCGAC
  TATGTGAGCTGAAAGACTGCTCTTCACTGACCGGCCGTGTCATAGAGCAGCGCATCCCCTACCGTGGCAC
  CCGCTACCCAGAGGTTAACAGGCGCATTGAGCGGCTCATCAACAAGCCAGGGCCTGATACCTTCCCTGAC
  TATGGGGATGTGCTTCGGGCTGTAGAGAAGGCAGCTGCCCGACACAGCCTTGGCCTCCCCCGACAGCAGC
  TCCAGCTCATGGCTCAGGATGCCTTCCGAGATGTGGGCATCAGGTTACAGGAGCGACGTCACCTCGATCT
  CATCTACAACTTTGGCTGCCACCTCACAGATGACTATAGGCCAGGCGTTGACCCTGCACTATCAGATCCT
  GTGTTGGCCCGGCGCCTTCGGGAAAACCGGAGTTTGGCCATGAGTCGGCTGGATGAGGTCATCTCCAAAT
  ATGCAATGTTGCAAGACAAAAGTGAGGAGGGCGAGAGAAAAAAGAGAAGAGCTCGGCTCCAAGGCACCTC
  TTCCCACTCTGCAGACACCCCCGAAGCCTCCTTGGATTCTGGTGAGGGCCCTAGTGGAATGGCATCCCAG
  GGGTGCCCTTCTGCCTCCAGAGCTGAGACAGATGACGAAGACGATGAGGAGAGTGATGAGGAAGAGGAGG
  AGGAGGAGGAAGAAGAAGAGGAGGAGGCCACAGATTCTGAAGAGGAGGAGGATCTGGAACAGATGCAGGA
  GGGTCAGGAGGATGATGAAGAGGAGGACGAAGAGGAAGAAGCAGCAGCAGGTAAAGATGGAGACAAGAGC
  CCCATGTCCTCACTACAGATCTCCAATGAAAAGAACCTGGAACCTGGCAAACAGATCAGCAGATCTTCAG
  GGGAGCAGCAAAACAAAGGACGCATAGTGTCACCATCGTTACTGTCAGAAGAACCCCTGGCCCCCTCCAG
  CATAGATGCTGAAAGCAATGGAGAACAGCCTGAGGAGCTGACCCTGGAGGAAGAAAGCCCTGTGTCTCAG
  CTCTTTGAGCTAGAGATTGAAGCTTTGCCCCTGGATACCCCTTCCTCTGTGGAGACGGACATTTCCTCTT
  CCAGGAAGCAATCAGAGGAGCCCTTCACCACTGTCTTAGAGAATGGAGCAGGCATGGTCTCTTCTACTTC
  CTTCAATGGAGGCGTCTCTCCTCACAACTGGGGAGATTCTGGTCCCCCCTGCAAAAAATCTCGGAAGGAG
  AAGAAGCAAACAGGATCAGGGCCATTAGGAAACAGCTATGTGGAAAGGCAAAGGTCAGTGCATGAGAAGA
  ATGGGAAAAAGATATGTACCCTGCCCAGCCCACCTTCCCCCTTGGCTTCCTTGGCCCCAGTTGCTGATTC
  CTCCACGAGGGTGGACTCTCCCAGCCATGGCCTGGTGACCAGCTCCCTCTGCATCCCTTCTCCAGCCCGG
  CTGTCCCAAACCCCCCATTCACAGCCTCCTCGGCCTGGTACTTGCAAGACAAGTGTGGCCACACAATGCG
  ATCCAGAAGAGATCATCGTGCTCTCAGACTCTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234672 representing NM_001254717
  Red=Cloning site Green=Tags(s)
  
  MQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNL
  APAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSEL
  DDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPD
  YGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDP
  VLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQ
  GCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKS
  PMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQ
  LFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKE
  KKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPAR
  LSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001254717
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001254717.1, NP_001241646.1
• RefSeq Size: 2369 bp
• RefSeq ORF: 1998 bp
• Locus ID: 1616
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway
• MW: 74 kDa
• Gene Summary: 'This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]'