CARD14 (NM_001257970) Human Tagged ORF Clone

CAT#: RC234804

  • TrueORF®

CARD14 (Myc-DDK tagged) - Homo sapiens caspase recruitment domain family, member 14 (CARD14), transcript variant 3


  "NM_001257970" in other vectors (2)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CARD14
Synonyms BIMP2; CARMA2; PRP; PSORS2; PSS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC234804 representing NM_001257970
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAACTGTGCCGCAGGGACTCCGCACTCACGGCACTGGACGAGGAGACACTGTGGGAGATGATGG
AGAGCCACCGCCACAGGATCGTACGCTGCATCTGCCCCAGCCGCCTCACCCCCTACCTGCGCCAGGCCAA
GGTGCTGTGCCAGCTGGACGAGGAGGAGGTGCTGCACAGCCCCCGGCTCACCAACAGCGCCATGCGGGCC
GGGCACTTGCTGGATTTGCTGAAGACTCGAGGGAAGAACGGGGCCATCGCCTTCCTGGAGAGCCTGAAGT
TCCACAACCCTGACGTCTACACCCTGGTCACCGGGCTGCAGCCTGATGTTGACTTCAGTAACTTTAGCGG
TCTCATGGAGACATCCAAGCTGACCGAGTGCCTGGCTGGGGCCATCGGCAGCCTGCAGGAGGAGCTGAAC
CAGGAAAAGGGGCAGAAGGAGGTGCTGCTGCGGCGGTGCCAGCAGCTGCAGGAGCACCTGGGCCTGGCCG
AGACCCGTGCCGAGGGCCTGCACCAGCTGGAGGCTGACCACAGCCGCATGAAGCGTGAGGTTAGCGCACA
CTTCCATGAGGTGCTGAGGCTGAAGGACGAGATGCTCAGCCTCTCGCTGCACTATAGCAATGCGCTGCAG
GAGAAGGAGCTGGCCGCCTCACGCTGCCGCAGCCTGCAGGAGGAGCTGTATCTACTGAAGCAGGAGCTGC
AGCGAGCCAACATGGTTTCCTCCTGTGAGCTGGAATTGCAAGAGCAGTCCCTGAGGACAGCCAGCGACCA
GGAGTCCGGGGATGAGGAGCTGAACCGCCTGAAGGAGGAGAATGAGAAACTGCGCTCGCTGACTTTCAGC
CTGGCGGAGAAGGACATTCTGGAGCAGAGCCTGGACGAGGCGCGGGGGAGCCGACAGGAGCTGGTGGAGC
GCATCCACTCGCTGCGGGAGCGGGCCGTGGCTGCCGAGAGGCAGCGAGAGCAGTACTGGGAAGAGAAGGA
ACAGACCCTGCTGCAGTTCCAGAAGAGTAAGATGGCCTGCCAACTCTACAGGGAGAAGGTGAATGCGCTG
CAGGCCCAGGTGTGCGAGCTGCAGAAGGAGCGAGACCAGGCGTACTCCGCGAGGGACAGTGCTCAGAGGG
AGATTTCCCAGAGCCTGGTGGAGAAGGACTCCCTCCGCAGGCAGGTGTTCGAGCTGACGGACCAGGTCTG
CGAGCTGCGCACACAGCTTCGCCAGCTGCAGGCAGAGCCTCCGGGTGTGCTCAAGCAGGAAGCCAGGACC
AGGGAGCCCTGTCCACGGGAGAAGCAGCGGCTGGTGCGGATGCATGCCATCTGCCCCAGAGACGACAGCG
ACTGCAGCCTCGTCAGCTCCACAGAGTCTCAGCTCTTGTCGGACCTGAGTGCCACGTCCAGCCGCGAGCT
GGTGGACAGCTTCCGCTCCAGCAGCCCCGCGCCCCCCAGCCAGCAGTCCCTGTACAAGCGGGTGGCCGAG
GACTTCGGGGAAGAACCCTGGTCTTTCAGCAGCTGCCTGGAGATCCCGGAGGGAGACCCGGGAGCCCTGC
CGGGAGCTAAGGCAGGCGACCCACACCTGGATTATGAGCTCCTAGACACGGCAGACCTTCCGCAGCTGGA
AAGCAGCCTGCAGCCAGTCTCCCCTGGAAGGCTTGATGTCTCGGAGAGCGGCGTCCTCATGCGGCGGAGG
CCAGCCCGCAGGATCCTGAGCCAGGTCACCATGCTGGCGTTCCAGGGGGATGCATTGCTGGAGCAGATCA
GCGTCATCGGCGGGAACCTCACGGGCATCTTCATCCACCGGGTCACCCCGGGCTCGGCGGCGGACCAGAT
GGCCTTGCGCCCGGGCACCCAGATTGTGATGGTTGATTACGAAGCCTCAGAGCCCTTGTTCAAGGCAGTC
CTGGAGGACACGACCCTGGAGGAGGCCGTGGGGCTTCTCAGGAGGGTGGACGGCTTCTGCTGCCTGTCTG
TGAAGGTCAACACGGACGGTTATAAGAGGCTACTCCAGGACCTGGAGGCCAAAGTGGCGACCTCGGGGGA
CTCATTCTACATCCGGGTCAACCTGGCCATGGAGGGCAGGGCCAAAGGGGAGCTGCAGGTGCATTGCAAC
GAGGTCCTGCACGTCACCGACACCATGTTCCAGGGCTGCGGCTGCTGGCATGCCCACCGCGTGAACTCTT
ACACCATGAAGGATACTGCCGCGCACGGCACCATCCCCAACTACTCCAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC234804 representing NM_001257970
Red=Cloning site Green=Tags(s)

MGELCRRDSALTALDEETLWEMMESHRHRIVRCICPSRLTPYLRQAKVLCQLDEEEVLHSPRLTNSAMRA
GHLLDLLKTRGKNGAIAFLESLKFHNPDVYTLVTGLQPDVDFSNFSGLMETSKLTECLAGAIGSLQEELN
QEKGQKEVLLRRCQQLQEHLGLAETRAEGLHQLEADHSRMKREVSAHFHEVLRLKDEMLSLSLHYSNALQ
EKELAASRCRSLQEELYLLKQELQRANMVSSCELELQEQSLRTASDQESGDEELNRLKEENEKLRSLTFS
LAEKDILEQSLDEARGSRQELVERIHSLRERAVAAERQREQYWEEKEQTLLQFQKSKMACQLYREKVNAL
QAQVCELQKERDQAYSARDSAQREISQSLVEKDSLRRQVFELTDQVCELRTQLRQLQAEPPGVLKQEART
REPCPREKQRLVRMHAICPRDDSDCSLVSSTESQLLSDLSATSSRELVDSFRSSSPAPPSQQSLYKRVAE
DFGEEPWSFSSCLEIPEGDPGALPGAKAGDPHLDYELLDTADLPQLESSLQPVSPGRLDVSESGVLMRRR
PARRILSQVTMLAFQGDALLEQISVIGGNLTGIFIHRVTPGSAADQMALRPGTQIVMVDYEASEPLFKAV
LEDTTLEEAVGLLRRVDGFCCLSVKVNTDGYKRLLQDLEAKVATSGDSFYIRVNLAMEGRAKGELQVHCN
EVLHVTDTMFQGCGCWHAHRVNSYTMKDTAAHGTIPNYSR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001257970
ORF Size 2220 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_001257970.1, NP_001244899.1
RefSeq Size 2612
RefSeq ORF 2223
Locus ID 79092
Protein Families Druggable Genome
MW 83.7 kDa
Gene Summary This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

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