APLP2 (NM_001243299) Human Tagged ORF Clone

CAT#: RC234850

APLP2 (Myc-DDK tagged) - Homo sapiens amyloid beta (A4) precursor-like protein 2 (APLP2), transcript variant 7

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APLP2
• Synonyms: APLP-2; APPH; APPL2; CDEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234850 representing NM_001243299
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGAGCCCCGGGGGAGCTCCCGCGCCAGGCCGCCCGCTGCTCCCTCTGCCGCCTGGGGCCGGGTC
  GCGGACGCGCATTTTTTAAGTGGCGCTGTTTGCCTGCGTCCGTAGACCGAGGAAACCCGCTCTGGGCTCT
  TGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCAATGTTTTGTGGGAAGTTAAAT
  ATGCATGTGAACATTCAGACTGGGAAATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAA
  AAGAAGAAGTTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAA
  CCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCT
  TTCAAGTGTCTCGTGGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCACA
  AAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCA
  GGGAATGACCTTATATAGCTACGGCATGCTGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATAT
  GTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAG
  AGGAAGAGGAAGATGAAGAGGAAGACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGA
  AGACTTCACAGAAGCAGCTGTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGAC
  CGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGAATCCTACTGAACCCGGCAGCG
  ACGGCACCATGTCAGACAAGGAAATTACTCATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGG
  GCCCTGCCGGGCCGTGATGCCTCGTTGGTACTTCGACCTCTCCAAGGGAAAGTGCGTGCGCTTTATATAT
  GGTGGCTGCGGCGGCAACAGGAACAATTTTGAGTCTGAGGATTATTGTATGGCTGTGTGTAAAGCGATGA
  TTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCA
  TGCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAG
  GAATGGGAAGAGGCAGAGCTTCAAGCTAAGAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACT
  TCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCACCT
  GGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAG
  TCTGACCCGCCACGGCCTCATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATC
  GCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGAAAAGGCGGCCCAGATGAAATC
  CCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTA
  CCTTATGTAGCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACC
  AGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATCCC
  ACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGAT
  GGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTG
  ACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGA
  ATCCGTGGGCCCACTGCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCA
  GTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATCAGCC
  ACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGG
  CTATGAGAACCCCACCTACAAATACCTGGAGCAGATGCAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234850 representing NM_001243299
  Red=Cloning site Green=Tags(s)
  
  MLRAPGELPRQAARCSLCRLGPGRGRAFFKWRCLPASVDRGNPLWALAANAGTGFAVAEPQIAMFCGKLN
  MHVNIQTGKWEPDPTGTKSCFETKEEVLQYCQEMYPELQITNVMEANQRVSIDNWCRRDKKQCKSRFVTP
  FKCLVGEFVSDVLLVPEKCQFFHKERMEVCENHQHWHTVVKEACLTQGMTLYSYGMLLPCGVDQFHGTEY
  VCCPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAAVDEDDEDEEEGEEVVED
  RDYYYDTFKGDDYNEENPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIY
  GGCGGNRNNFESEDYCMAVCKAMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKK
  EWEEAELQAKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRMALENYLAALQ
  SDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKV
  PYVAQEIQEEIDELLQEQRADMDQFTASISETPVDVRVSSEESEEIPPFHPFHPFPALPENEGSGVGEQD
  GGLIGAEEKVINSKNKVDENMVIDETLDVKEMIFNAERVGGLEEERESVGPLREDFSLSSSALIGLLVIA
  VAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243299
• ORF Size: 2283 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243299.1, NP_001230228.1
• RefSeq Size: 3709 bp
• RefSeq ORF: 2286 bp
• Locus ID: 334
• Cytogenetics: 11q24.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 87.7 kDa
• Gene Summary: 'This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]'