LONP1 (NM_001276480) Human Tagged ORF Clone

CAT#: RC234853

LONP1 (Myc-DDK tagged) - Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LONP1
• Synonyms: CODASS; hLON; LON; LonHS; LONP; PIM1; PRSS15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234853 representing NM_001276480
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGACCTTGGGGACAAGCTGCGCATGATCGTCATGGGACACAGAAGAGTCCATATCAGCAGACAGC
  TGGAGGTGGAGCCCGAGGAGCCGGAGGCGGAGAACAAGCACAAGCCCCGCAGGAAGTCAAAGCGGGGCAA
  GAAGGAGGCGGAGGACGAGCTGAGCGCCAGGCACCCGGCGGAGCTGGCGATGGAGCCCACCCCTGAGCTC
  CCGGCTGAGGTGCTCATGGTGGAGGTAGAGAACGTTGTCCACGAGGACTTCCAGGTCACGGAGGAGGTGA
  AAGCCCTGACTGCAGAGATCGTGAAGACCATCCGGGACATCATTGCCTTGAACCCTCTCTACAGGGAGTC
  AGTGCTGCAGATGATGCAGGCTGGCCAGCGGGTGGTGGACAACCCCATCTACCTGAGCGACATGGGCGCC
  GCGCTCACCGGGGCCGAGTCCCATGAGCTGCAGGACGTCCTGGAAGAGACCAATATTCCTAAGCGGCTGT
  ACAAGGCCCTCTCCCTGCTGAAGAAGGAATTTGAACTGAGCAAGCTGCAGCAGCGCCTGGGGCGGGAGGT
  GGAGGAGAAGATCAAGCAGACCCACCGTAAGTACCTGCTGCAGGAGCAGCTAAAGATCATCAAGAAGGAG
  CTGGGCCTGGAGAAGGACGACAAGGATGCCATCGAGGAGAAGTTCCGGGAGCGCCTGAAGGAGCTCGTGG
  TCCCCAAGCACGTCATGGATGTTGTGGACGAGGAGCTGAGCAAGCTGGGCCTGCTGGACAACCACTCCTC
  GGAGTTCAATGTCACCCGCAACTACCTAGACTGGCTCACGTCCATCCCTTGGGGCAAGTACAGCAACGAG
  AACCTGGACCTGGCGCGGGCACAGGCAGTGCTGGAGGAAGACCACTACGGCATGGAGGACGTCAAGAAAC
  GCATCCTGGAGTTCATTGCCGTTAGCCAGCTCCGCGGCTCCACCCAGGGCAAGATCCTCTGCTTCTATGG
  CCCCCCTGGCGTGGGTAAGACCAGCATTGCTCGCTCCATCGCCCGCGCCCTGAACCGAGAGTACTTCCGC
  TTCAGCGTCGGGGGCATGACTGACGTGGCTGAGATCAAGGGCCACAGGCGGACCTACGTGGGCGCCATGC
  CCGGGAAGATCATCCAGTGTTTGAAGAAGACCAAGACGGAGAACCCCCTGATCCTCATCGACGAGGTGGA
  CAAGATCGGCCGAGGCTACCAGGGGGACCCGTCGTCGGCACTGCTGGAGCTGCTGGACCCAGAGCAGAAT
  GCCAACTTCCTGGACCACTACCTGGACGTGCCCGTGGACTTGTCCAAGGTGCTGTTCATCTGCACGGCCA
  ACGTCACGGACACCATCCCCGAGCCGCTGCGAGACCGTATGGAGATGATCAACGTGTCGGGCTACGTGGC
  CCAGGAGAAGCTGGCCATTGCGGAGCGCTACCTGGTGCCCCAGGCTCGCGCCCTGTGTGGCTTGGATGAG
  AGCAAGGCCAAGCTGTCATCGGACGTGCTGACGCTGCTCATCAAGCAGTACTGCCGCGAGAGCGGTGTCC
  GCAACCTGCAGAAGCAAGTGGAGAAGGTGTTACGGAAATCGGCCTACAAGATTGTCAGCGGCGAGGCCGA
  GTCCGTGGAGGTGACGCCCGAGAACCTGCAGGACTTCGTGGGGAAGCCCGTGTTCACCGTGGAGCGCATG
  TATGACGTGACACCGCCCGGCGTGGTCATGGGGCTGGCCTGGACCGCAATGGGAGGCTCCACGCTGTTTG
  TGGAGACATCCCTGAGACGGCCACAGGACAAGGATGCCAAGGGTGACAAGGATGGCAGCCTGGAGGTGAC
  AGGCCAGCTGGGGGAGGTGATGAAGGAGAGCGCCCGCATAGCCTACACCTTCGCCAGAGCCTTCCTCATG
  CAGCACGCCCCCGCCAATGACTACCTGGTGACCTCACACATCCACCTGCATGTGCCCGAGGGCGCCACCC
  CCAAGGACGGCCCAAGCGCAGGCTGCACCATCGTCACGGCCCTGCTGTCCCTGGCCATGGGCAGGCCTGT
  CCGGCAGAATCTGGCCATGACTGGCGAAGTCTCCCTCACGGGCAAGATCCTGCCTGTTGGTGGCATCAAG
  GAGAAGACCATTGCGGCCAAGCGCGCAGGGGTGACGTGCATCGTCCTGCCAGCCGAGAACAAGAAGGACT
  TCTACGACCTGGCAGCCTTCATCACCGAGGGCCTGGAGGTGCACTTCGTGGAACACTACCGGGAGATCTT
  CGACATCGCCTTCCCGGACGAGCAGGCAGAGGCGCTGGCCGTGGAACGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234853 representing NM_001276480
  Red=Cloning site Green=Tags(s)
  
  MQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPEL
  PAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGA
  ALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKE
  LGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNE
  NLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFR
  FSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQN
  ANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDE
  SKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERM
  YDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLM
  QHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIK
  EKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276480
• ORF Size: 2289 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001276480.1, NP_001263409.1
• RefSeq Size: 2900
• RefSeq ORF: 2292
• Locus ID: 9361
• Protein Families: Druggable Genome, Protease
• MW: 85.6 kDa
• Gene Summary: This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]