BCAR3 (NM_001261408) Human Tagged ORF Clone

CAT#: RC234936

BCAR3 (Myc-DDK tagged) - Homo sapiens breast cancer anti-estrogen resistance 3 (BCAR3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCAR3
• Synonyms: AND-34; NSP2; SH2D3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234936 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGGAAAATTTGCAAGCCTTCCCAGAAACATGCCGGTGAATCACCAGTTCCCCCTGGCCTCAT
  CCATGGACCTTCTGAGCAGCAGGTCCCCTCTCGCTGAGCATCGCCCAGATGCCTATCAAGATGTGTCTAT
  ACATGGCACCCTTCCACGGAAGAAAAAAGGTCCTCCTCCCATAAGGTCCTGTGATGACTTCAGTCACATG
  GGCACCCTCCCCCACTCCAAATCCCCACGGCAGAACTCGCCTGTGACCCAGGATGGCATCCAGGAGAGCC
  CATGGCAGGACCGGCACGGCGAAACCTTCACCTTCAGGGATCCACATCTTCTGGACCCAACTGTGGAATA
  TGTGAAGTTCTCCAAGGAGAGGCACATCATGGACAGGACCCCCGAGAAACTGAAGAAGGAGCTGGAGGAG
  GAGCTGCTCCTGAGCAGCGAGGACCTGCGCAGCCATGCCTGGTACCACGGCCGCATCCCCCGACAGGTGT
  CTGAAAACCTTGTGCAGCGAGATGGTGACTTCCTAGTTCGTGACTCTCTGTCCAGCCCTGGGAACTTTGT
  CCTGACCTGTCAGTGGAAGAACCTCGCTCAGCACTTCAAAATCAACCGGACAGTTCTGCGACTCAGCGAG
  GCCTACAGCCGCGTGCAGTACCAGTTCGAGATGGAGAGCTTCGACTCCATCCCCGGCCTGGTGCGCTGCT
  ACGTGGGCAACCGCCGGCCCATCTCCCAGCAGAGTGGCGCCATCATCTTCCAGCCCATCAACAGGACGGT
  GCCTCTGCGGTGCCTGGAGGAGCATTATGGCACCTCCCCAGGCCAGGCCCGGGAGGGCAGCCTCACCAAG
  GGAAGGCCGGATGTGGCCAAGAGGCTGAGCCTCACCATGGGTGGCGTCCAGGCCCGAGAGCAGAATTTGC
  CCAGGGGAAACCTCCTCAGAAACAAAGAAAAGAGTGGTAGCCAGCCCGCCTGCCTGGATCACATGCAGGA
  CAGAAGAGCCTTGTCCCTCAAAGCCCACCAGTCAGAGAGCTACCTGCCGATTGGCTGCAAGCTGCCACCT
  CAGTCCTCGGGTGTGGACACAAGCCCCTGCCCAAACTCACCTGTGTTCAGGACGGGAAGCGAGCCTGCCC
  TGAGCCCAGCAGTGGTTCGGAGGGTCTCCTCAGACGCCAGGGCTGGGGAGGCGCTGAGGGGATCAGACAG
  TCAACTGTGCCCTAAGCCCCCGCCTAAGCCCTGCAAGGTGCCGTTCCTCAAGGTTCCCTCGTCTCCCTCT
  GCCTGGCTCAACTCAGAGGCCAACTACTGTGAACTGAACCCAGCGTTTGCCACAGGCTGCGGCAGGGGAG
  CAAAGCTACCCTCATGTGCCCAGGGAAGCCACACAGAACTGCTCACAGCCAAGCAGAATGAGGCGCCAGG
  TCCCCGGAACTCTGGCGTCAACTACTTGATCCTTGATGATGATGACAGGGAAAGACCTTGGGAACCTGCG
  GCAGCTCAGATGGAGAAGGGGCAGTGGGACAAGGGCGAGTTTGTGACGCCCCTCCTGGAGACTGTCTCCT
  CCTTCAGGCCCAACGAGTTTGAGTCAAAGTTCCTTCCCCCTGAGAATAAGCCCCTGGAAACAGCAATGTT
  GAAACGTGCAAAAGAACTGTTCACCAACAACGACCCCAAGGTCATCGCCCAGCACGTACTGAGCATGGAC
  TGCAGGGTTGCTAGGATACTTGGAGTCTCTGAAGAGATGAGGAGGAACATGGGGGTGAGCTCAGGCCTGG
  AACTCATTACCTTGCCTCACGGACACCAGCTGCGCCTGGACATAATTGAAAGACACAACACAATGGCCAT
  CGGCATTGCAGTGGACATTCTGGGATGCACGGGCACTTTGGAGGACCGAGCGGCCACTCTGAGTAAGATC
  ATCCAGGTGGCGGTGGAACTGAAGGATTCCATGGGGGACCTCTATTCCTTCTCAGCTCTCATGAAAGCCC
  TGGAAATGCCACAGATCACAAGGTTAGAAAAGACGTGGACTGCTCTGCGGCACCAGTACACCCAAACTGC
  CATTCTCTATGAGAAACAGCTGAAGCCCTTCAGCAAACTCCTGCATGAAGGCAGAGAGTCCACATGTGTT
  CCCCCAAACAATGTATCAGTCCCACTGCTGATGCCGCTTGTGACGTTAATGGAGCGCCAGGCTGTGACTT
  TTGAAGGAACCGACATGTGGGAAAAAAACGACCAGAGCTGTGAAATCATGCTGAACCATTTGGCAACAGC
  GCGATTCATGGCCGAGGCTGCAGACAGCTACCGGATGAATGCTGAGAGGATCCTGGCAGGTTTTCAACCA
  GATGAAGAAATGAATGAAATCTGCAAGACTGAATTTCAAATGCGATTGCTATGGGGCAGCAAAGGTGCAC
  AAGTCAATCAGACAGAGAGATATGAGAAATTCAACCAGATTTTAACTGCCCTCTCGCGTAAATTGGAACC
  TCCTCCTGTAAAGCAGGCAGAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234936 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAGKFASLPRNMPVNHQFPLASSMDLLSSRSPLAEHRPDAYQDVSIHGTLPRKKKGPPPIRSCDDFSHM
  GTLPHSKSPRQNSPVTQDGIQESPWQDRHGETFTFRDPHLLDPTVEYVKFSKERHIMDRTPEKLKKELEE
  ELLLSSEDLRSHAWYHGRIPRQVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSE
  AYSRVQYQFEMESFDSIPGLVRCYVGNRRPISQQSGAIIFQPINRTVPLRCLEEHYGTSPGQAREGSLTK
  GRPDVAKRLSLTMGGVQAREQNLPRGNLLRNKEKSGSQPACLDHMQDRRALSLKAHQSESYLPIGCKLPP
  QSSGVDTSPCPNSPVFRTGSEPALSPAVVRRVSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPSSPS
  AWLNSEANYCELNPAFATGCGRGAKLPSCAQGSHTELLTAKQNEAPGPRNSGVNYLILDDDDRERPWEPA
  AAQMEKGQWDKGEFVTPLLETVSSFRPNEFESKFLPPENKPLETAMLKRAKELFTNNDPKVIAQHVLSMD
  CRVARILGVSEEMRRNMGVSSGLELITLPHGHQLRLDIIERHNTMAIGIAVDILGCTGTLEDRAATLSKI
  IQVAVELKDSMGDLYSFSALMKALEMPQITRLEKTWTALRHQYTQTAILYEKQLKPFSKLLHEGRESTCV
  PPNNVSVPLLMPLVTLMERQAVTFEGTDMWEKNDQSCEIMLNHLATARFMAEAADSYRMNAERILAGFQP
  DEEMNEICKTEFQMRLLWGSKGAQVNQTERYEKFNQILTALSRKLEPPPVKQAEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001261408
• ORF Size: 2475 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001261408.1, NP_001248337.1
• RefSeq Size: 3240 bp
• RefSeq ORF: 2478 bp
• Locus ID: 8412
• Protein Families: Druggable Genome
• MW: 92.6 kDa
• Gene Summary: Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]