ARMCX5-GPRASP2 (NM_001199818) Human Tagged ORF Clone

CAT#: RC234944

ARMCX5 (Myc-DDK tagged) - Homo sapiens ARMCX5-GPRASP2 readthrough (ARMCX5-GPRASP2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARMCX5-GPRASP2
• Synonyms: ARMCX5-GPRASP2-BHLHB9-LINC00630; bHLHb9; p60TRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234944 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGACTGGGGCAGAGATTGAGCCTAGTGCCCAGGCCAAGCCTGAAAAGAAGGCTGGGGAAGAGGTTAT
  CGCTGGGCCTGAGAGAGAGAATGATGTCCCTCTGGTGGTCAGACCCAAGGTTAGGACCCAGGCAACTACT
  GGGGCAAGGCCCAAAACTGAGACCAAGTCTGTGCCTGCGGCAAGGCCCAAAACTGAGGCCCAAGCAATGT
  CTGGGGCAAGGCCCAAAACTGAGGTCCAAGTAATGGGTGGTGCAAGACCCAAAACGGAGGCTCAAGGAAT
  CACAGGGGCCAGGCCCAAAACCGATGCCAGGGCAGTAGGTGGCGCTCGTTCTAAAACTGATGCCAAGGCA
  ATCCCTGGAGCAAGGCCCAAGGATGAGGCCCAGGCATGGGCCCAGAGTGAATTTGGGACTGAAGCAGTGT
  CACAGGCAGAAGGAGTGTCCCAGACTAATGCCGTTGCTTGGCCACTGGCCACTGCTGAGTCTGGATCAGT
  TACTAAATCTAAGGGCCTGTCTATGGATAGAGAACTAGTCAATGTGGATGCTGAAACCTTTCCTGGCACC
  CAGGGTCAGAAAGGAATCCAGCCCTGGTTTGGACCAGGGGAGGAGACTAATATGGGGTCTTGGTGCTATT
  CCAGGCCCAGGGCCAGAGAGGAGGCCTCTAATGAGTCTGGGTTCTGGTCAGCAGATGAGACCTCTACAGC
  GTCTTCTTTCTGGACTGGAGAAGAGACAAGTGTCAGATCATGGCCCAGGGAAGAGTCCAATACCAGGTCC
  AGGCACAGGGCTAAACATCAGACTAATCCCAGGTCCAGGCCCAGATCCAAGCAAGAAGCCTATGTTGATT
  CCTGGTCTGGATCTGAGGATGAGGCCAGCAACCCATTCTCCTTCTGGGTTGGAGAAAATACCAATAACTT
  GTTCAGGCCCAGAGTCAGGGAGGAGGCAAATATCAGGTCCAAGCTCAGGACAAATAGAGAAGATTGTTTT
  GAATCTGAGTCTGAAGATGAGTTCTATAAGCAGTCCTGGGTTTTGCCTGGAGAAGAGGCCAATAGTAGAT
  TCAGGCACAGAGACAAAGAAGATCCTAATACTGCCTTGAAACTCAGGGCCCAGAAAGATGTTGACAGTGA
  TAGGGTCAAACAAGAACCCAGGTTTGAGGAGGAAGTCATTATTGGGTCCTGGTTCTGGGCAGAAAAAGAG
  GCCAGTTTGGAGGGTGGAGCTTCAGCAATCTGTGAATCTGAGCCAGGAACTGAGGAGGGGGCCATTGGCG
  GATCCGCGTACTGGGCTGAGGAAAAGTCCAGTTTGGGGGCTGTGGCCAGAGAAGAGGCCAAGCCGGAGTC
  TGAAGAAGAGGCCATATTTGGGTCCTGGTTCTGGGACAGAGATGAGGCCTGCTTTGACCTAAATCCCTGT
  CCTGTGTACAAGGTCAGTGATAGGTTCAGAGATGCAGCTGAGGAGCTTAATGCATCCTCCAGGCCCCAAA
  CCTGGGACGAGGTCACTGTTGAATTCAAACCTGGTCTTTTTCATGGGGTTGGCTTCCGATCCACAAGCCC
  CTTTGGAATTCCCGAAGAGGCTTCTGAAATGCTTGAGGCAAAGCCCAAGAACCTGGAACTTAGCCCAGAA
  GGAGAAGAGCAGGAATCTTTGCTTCAGCCTGATCAGCCTAGTCCTGAGTTCACATTTCAGTATGATCCTT
  CCTACCGGTCAGTCCGGGAAATTCGAGAGCATCTTAGGGCCAGGGAGAGTGCAGAGTCTGAGAGTTGGTC
  ATGCAGCTGCATACAATGTGAGCTGAAAATTGGTTCTGAAGAGTTTGAAGAATTCCTTTTATTAATGGAC
  AAAATTCGGGATCCTTTTATTCATGAAATATCTAAAATTGCAATGGGTATGAGAAGTGCTTCTCAATTTA
  CCCGAGATTTCATTCGAGATTCAGGTGTTGTCTCACTTATTGAAACCTTGCTTAATTATCCATCCTCTAG
  AGTTAGGACAAGTTTTTTGGAAAATATGATTCACATGGCTCCACCTTATCCAAATCTAAACATGATTGAG
  ACATTCATATGTCAAGTGTGTGAGGAAACCCTTGCACATAGTGTGGATTCCCTTGAGCAGCTGACTGGAA
  TAAGGATGCTTAGACACCTCACTATGACTATTGACTATCACACACTGATTGCCAACTATATGTCCGGGTT
  TCTCTCCTTATTAACCACAGCCAATGCGAGAACGAAGTTTCACGTTCTGAAAATGCTATTGAATTTGTCT
  GAAAATCCTGCTGTGGCAAAAAAACTATTCAGTGCCAAAGCTCTTTCAATATTTGTGGGTCTCTTTAACA
  TAGAAGAGACAAATGATAATATTCAAATTGTTATTAAAATGTTTCAGAATATCAGTAACATTATAAAAAG
  TGGAAAGATGTCCTTAATTGATGATGATTTCAGTCTTGAGCCGCTTATTTCTGCATTTCGTGAATTTGAG
  GAGTTAGCTAAGCAACTACAAGCCCAAATAGACAACCAAAATGATCCTGAGGTGGGACAACAAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234944 protein sequence
  Red=Cloning site Green=Tags(s)
  
  X*LGQRLSLVPRPSLKRRLGKRLSLGLRERMMSLWWSDPRLGPRQLLGQGPKLRPSLCLRQGPKLRPKQC
  LGQGPKLRSK*WVVQDPKRRLKESQGPGPKPMPGQ*VALVLKLMPRQSLEQGPRMRPRHGPRVNLGLKQC
  HRQKECPRLMPLLGHWPLLSLDQLLNLRACLWIEN*SMWMLKPFLAPRVRKESSPGLDQGRRLIWGLGAI
  PGPGPERRPLMSLGSGQQMRPLQRLLSGLEKRQVSDHGPGKSPIPGPGTGLNIRLIPGPGPDPSKKPMLI
  PGLDLRMRPATHSPSGLEKIPITCSGPESGRRQISGPSSGQIEKIVLNLSLKMSSISSPGFCLEKRPIVD
  SGTETKKILILP*NSGPRKMLTVIGSNKNPGLRRKSLLGPGSGQKKRPVWRVELQQSVNLSQELRRGPLA
  DPRTGLRKSPVWGLWPEKRPSRSLKKRPYLGPGSGTEMRPALT*IPVLCTRSVIGSEMQLRSLMHPPGPK
  PGTRSLLNSNLVFFMGLASDPQAPLEFPKRLLKCLRQSPRTWNLAQKEKSRNLCFSLISLVLSSHFSMIL
  PTGQSGKFESILGPGRVQSLRVGHAAAYNVS*KLVLKSLKNSFY*WTKFGILLFMKYLKLQWV*EVLLNL
  PEISFEIQVLSHLLKPCLIIHPLELGQVFWKI*FTWLHLIQI*T*LRHSYVKCVRKPLHIVWIPLSS*LE
  *GCLDTSL*LLTITH*LPTICPGFSPY*PQPMRERSFTF*KCY*ICLKILLWQKNYSVPKLFQYLWVSLT
  *KRQMIIFKLLLKCFRISVTL*KVERCP*LMMISVLSRLFLHFVNLRS*LSNYKPK*TTKMILRWDNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199818
• ORF Size: 2514 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199818.1, NP_001186747.1
• RefSeq Size: 3940 bp
• RefSeq ORF: 2517 bp
• Locus ID: 100528062
• MW: 93.8 kDa
• Gene Summary: This locus represents naturally occurring readthrough transcription among the adjacent armadillo repeat containing, X-linked 5 (ARMCX5), G protein-coupled receptor associated sorting proteins 1 and 2 (GPRASP1 and GPRASP2), basic helix-loop-helix family member b9 (BHLHB9), and long intergenic non-protein coding RNA 630 (LINC00630) genes on chromosome X. Transcripts may make use of multiple alternative promoters and polyadenylation signals in this region. Readthrough transcripts may produce proteins identical to the proteins encoded by GPRASP2 or BHLHB9. [provided by RefSeq, Apr 2017]