ORC1 (NM_001190818) Human Tagged ORF Clone

CAT#: RC234972

  • TrueORF®

ORC1 (Myc-DDK tagged) - Homo sapiens origin recognition complex, subunit 1 (ORC1), transcript variant 2


  "NM_001190818" in other vectors (2)

Reconstitution Protocol

USD 750.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ORC1
Synonyms HSORC1; ORC1L; PARC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC234972 representing NM_001190818
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACACTACCCCACAAGGCTGAAGACCAGAAAAACTTATTCATGGGTTGGCAGGCCCTTGTTGGATC
GAAAACTGCACTACCAAACCTATAGAGAAATGTGTGTGAAAACAGAAGGTTGTTCCACCGAGATTCACAT
CCAGATTGGACAGTTTGTGTTGATTGAAGGGGATGATGATGAAAACCCGTATGTTGCTAAATTGCTTGAG
TTGTTCGAAGATGACTCTGATCCTCCTCCTAAGAAACGTGCTCGAGTACAGTGGTTTGTCCGATTCTGTG
AAGTCCCTGCCTGTAAACGGCATTTGTTGGGCCGGAAGCCTGGTGCACAGGAAATATTCTGGTATGATTA
CCCGGCCTGTGACAGCAACATTAATGCGGAGACCATCATTGGCCTTGTTCGGGTGATACCTTTAGCCCCA
AAGGATGTGGTACCGACGAATCTGAAAAATGAGAAGACACTCTTTGTGAAACTATCCTGGAATGAGAAGA
AATTCAGGCCACTTTCCTCAGAACTATTTGCGGAGTTGAATAAACCACAAGAGAGTGCAGCCAAGTGCCA
GAAACCCGTGAGAGCCAAGAGTAAGAGTGCAGAGAGCCCTTCTTGGACCCCAGCAGAACATGTGGCCAAA
AGGATTGAATCAAGGCACTCCGCCTCCAAATCTCGCCAAACTCCTACCCATCCTCTTACCCCAAGAGCCA
GAAAGAGGCTGGAGCTTGGCAACTTAGGTAACCCTCAGATGTCCCAGCAGACTTCATGTGCCTCCTTGGA
TTCTCCAGGAAGAATAAAACGGAAAGTGGCCTTCTCGGAGATCACCTCACCTTCTAAGAGATCTCAGCCT
GATAAACTTCAAACCTTGTCTCCAGCTCTGAAAGCCCCAGAGAAAACCAGAGAGACTGGACTCTCTTATA
CTGAGGATGACAAGAAGGCTTCACCTGAACATCGCATAATCCTGAGAACCCGAATTGCAGCTTCGAAAAC
CATAGACATTAGAGAGGAGAGAACACTTACCCCTATCAGTGGGGGACAGAGATCTTCAGTGGTGCCATCC
GTGATTCTGAAACCAGAAAACATCAAAAAGAGGGATGCAAAAGAAGCAAAAGCCCAGAATGAAGCGACCT
CTACTCCCCATCGTATCCGCAGAAAGAGTTCTGTCTTGACTATGAATCGGATTAGGCAGCAGCTTCGGTT
TCTAGGTAATAGTAAAAGTGACCAAGAAGAGAAAGAGATTCTGCCAGCAGCAGAGATTTCAGACTCTAGC
AGTGACGAAGAAGAGGCTTCCACACCGCCCCTTCCAAGGAGAGCACCCAGAACTGTGTCCAGGAACCTGC
GATCTTCCTTGAAGTCATCCTTACATACCCTCACGAAGGTGCCAAAGAAGAGTCTCAAGCCTAGAACGCC
ACGTTGTGCCGCTCCTCAGATCCGTAGTCGAAGCCTGGCTGCCCAGGAGCCAGCCAGTGTGCTGGAGGAA
GCCCGACTGAGGCTGCATGTTTCTGCTGTACCTGAGTCTCTTCCCTGTCGGGAACAGGAATTCCAAGACA
TCTACAATTTTGTGGAAAGCAAACTCCTTGACCATACCGGAGGGTGCATGTACATCTCCGGTGTCCCTGG
GACAGGGAAGACTGCCACTGTTCATGAAGTGATACGCTGCCTGCAGCAGGCAGCCCAAGCCAATGATGTT
CCTCCCTTTCAATACATTGAGGTCAATGGCATGAAGCTGACGGAGCCCCACCAAGTCTATGTGCAAATCT
TGCAGAAGCTAACAGGCCAAAAAGCAACAGCCAACCATGCGGCAGAACTGCTGGCAAAGCAATTCTGCAC
CCGAGGGTCACCTCAGGAAACCACCGTCCTGCTTGTGGATGAGCTCGACCTTCTGTGGACTCACAAACAA
GACATAATGTACAATCTCTTTGACTGGCCCACTCATAAGGAGGCCCGGCTTGTGGTCCTGGCAATTGCCA
ACACAATGGACCTGCCAGAGCGAATCATGATGAACCGGGTGTCCAGCCGACTGGGTCTTACCAGGATGTG
CTTCCAGCCCTATACATATAGCCAGCTGCAGCAGATCCTAAGGTCCCGGCTCAAGCATCTAAAGGCCTTT
GAAGATGATGCCATCCAGCTGGTAGCCAGGAAGGTAGCAGCACTGTCTGGAGATGCACGACGGTGCCTGG
ACATCTGCAGGCGTGCCACAGAGATCTGTGAGTTCTCCCAGCAGAAGCCTGACTCCCCTGGCCTGGTCAC
CATAGCCCACTCAATGGAAGCTGTGGATGAGATGTTTTCATCATCATACATCACGGCCATCAAAAATTCC
TCTGTTCTGGAACAGAGCTTCCTGAGAGCCATCCTCGCAGAGTTCCGTCGATCAGGACTGGAGGAAGCCA
CGTTTCAACAGATATATAGTCAACATGTGGCACTGTGCAGAATGGAGGGACTGCCGTACCCCACCATGTC
AGAGACCATGGCCGTGTGTTCTCACCTGGGCTCCTGTCGCCTCCTGCTTGTGGAGCCCAGCAGGAACGAT
CTGCTCCTTCGGGTGCGGCTCAACGTCAGCCAGGATGATGTGCTGTATGCGCTGAAAGACGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC234972 representing NM_001190818
Red=Cloning site Green=Tags(s)

MAHYPTRLKTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDDENPYVAKLLE
LFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQEIFWYDYPACDSNINAETIIGLVRVIPLAP
KDVVPTNLKNEKTLFVKLSWNEKKFRPLSSELFAELNKPQESAAKCQKPVRAKSKSAESPSWTPAEHVAK
RIESRHSASKSRQTPTHPLTPRARKRLELGNLGNPQMSQQTSCASLDSPGRIKRKVAFSEITSPSKRSQP
DKLQTLSPALKAPEKTRETGLSYTEDDKKASPEHRIILRTRIAASKTIDIREERTLTPISGGQRSSVVPS
VILKPENIKKRDAKEAKAQNEATSTPHRIRRKSSVLTMNRIRQQLRFLGNSKSDQEEKEILPAAEISDSS
SDEEEASTPPLPRRAPRTVSRNLRSSLKSSLHTLTKVPKKSLKPRTPRCAAPQIRSRSLAAQEPASVLEE
ARLRLHVSAVPESLPCREQEFQDIYNFVESKLLDHTGGCMYISGVPGTGKTATVHEVIRCLQQAAQANDV
PPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAELLAKQFCTRGSPQETTVLLVDELDLLWTHKQ
DIMYNLFDWPTHKEARLVVLAIANTMDLPERIMMNRVSSRLGLTRMCFQPYTYSQLQQILRSRLKHLKAF
EDDAIQLVARKVAALSGDARRCLDICRRATEICEFSQQKPDSPGLVTIAHSMEAVDEMFSSSYITAIKNS
SVLEQSFLRAILAEFRRSGLEEATFQQIYSQHVALCRMEGLPYPTMSETMAVCSHLGSCRLLLVEPSRND
LLLRVRLNVSQDDVLYALKDE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001190818
ORF Size 2583 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001190818.1, NP_001177747.1
RefSeq Size 3189 bp
RefSeq ORF 2586 bp
Locus ID 4998
Cytogenetics 1p32.3
Protein Families Stem cell - Pluripotency
Protein Pathways Cell cycle
MW 97.3 kDa
Gene Summary 'The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'

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