RALGDS (NM_001271774) Human Tagged ORF Clone
CAT#: RC234993
- TrueORF®
RALGDS (Myc-DDK tagged) - Homo sapiens ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 3
"NM_001271774" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RALGDS |
Synonyms | RalGEF; RGDS; RGF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234993 representing NM_001271774
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCTGTGGGGGCACTCCACAGCCCCTGCCCACACCCTCTCCTCCCCGCCTCTGCTGTTCTGCTCCC TGCCCTGTGCCCTCCACTTGCAGCCAGGGACTGGGCATCCACCCGGCCAGGTTCCCAGGAAGAGCTCCAC GCAGGAGATCGGTGAGGAGCTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCAC CACGGAGGCAACAAGGGGCAGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGAGACTT GCAAGGTGCGGACCGTGAAGGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGTGCCAGCCTTCCAGGG CAGCGACCTCTCCTACGTCACCATCTTCCTGTGTACCTATAGAGCCTTCACCACCACCCAACAGGTCCTG GACCTGCTGTTCAAAAGATACGGCTGCATCCTCCCCTATTCCGACGAGGATGGTGGACCCCAGGACCAAC TTAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTACTCGGAGGATTTCTGTCAACCTCC GGACTTTCCCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAACATGCCAGGCTCAGACCTGGAGCGC CGTGCCCACCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATTGAGGCAGAGCCTGAGGCTCTGTCAC CAGTGCCAGCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTAACACCAGCTCGAGCACCCAGCCCAGT GCCGGCTCCAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAGAGCTAGAAGTAGCTCCA GCACCAGCTCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATCGGCTCCAGCGCCAGCTC TGGAACTAGAGCCAGCTCCAGAACAGGATCCAGCTCCCTCACAAACTCTAGAGCTGGAGCCAGCTCCAGC ACCAGTTCCATCATTACAGCCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACGGGCTGAGTGAGGAGAAG CCTCACCTCTTGGTGTTCCCTCCAGATCTGGTGGCAGAGCAGTTTACACTGATGGATGCGGAACTGTTCA AGAAGGTGGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAGAAGGGCAAGGAGCACCT GGCGCCCACCATCCGCGCCACTGTCACCCAGTTCAACAGTGTGGCCAACTGTGTCATCACCACCTGCCTC GGGAACCGAAGCACGAAAGCCCCAGACAGGGCCAGGGTGGTGGAGCACTGGATCGAGGTGGCCAGGGAGT GCCGGATCCTCAAGAACTTCTCGTCACTGTATGCCATCCTCTCTGCCCTGCAGAGCAACTCCATCCACCG TCTGAAGAAGACGTGGGAAGACGTTTCCAGGGACAGTTTCCGGATCTTTCAGAAGCTGTCAGAGATCTTC TCAGATGAGAACAACTACTCATTGAGCCGGGAGCTGCTCATCAAGGAGGGCACCTCCAAGTTTGCCACCC TGGAGATGAACCCCAAGAGAGCCCAGAAACGGCCGAAGGAGACGGGCATCATCCAGGGCACCGTTCCCTA CCTGGGCACGTTCCTCACCGACCTGGTGATGCTGGACACTGCCATGAAGGACTATCTGTATGGCAGACTC ATCAACTTTGAGAAGAGGAGGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCTGCTGCAGTCGGCCTGCA ACAACTACAGCATCGCGCCAGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTGGAGCGGCTCAGCGAGAC TGAGAGCTACAACCTGTCGTGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGCAACACCCTCAGGACCAAG AAGAACACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCCCCCAGCACTGAGCTCAGTACCAGTGGCA GCTCCCACTCCAAGTCCTGTGACCAGCTCAGGTGTGGCCCCTACCTCAGCAGCGGGGACATCGCTGACGC GCTCAGCGTGCACTCGGCCGGCTCCTCTAGCTCCGACGTGGAGGAGATCAACATCAGCTTCGTCCCGGAG TCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAATCAGCCTCACAGTCATCCCCGGAGACCTCCGGCATCA GCTCAGCCTCCAGCAGCACCTCGTCCTCCTCAGCCTCCACCACGCCCGTGGCTGCCACACGCACCCACAA GCGCTCTGTCTCAGGGCTCTGCAACTCCAGCTCCGCGCTGCCGCTCTACAACCAGCAGGTGGGCGACTGC TGTATCATCCGCGTCAGCCTGGACGTGGACAATGGCAACATGTACAAGAGCATCCTGGTGACCAGCCAAG ATAAGGCTCCGGCTGTAATCCGCAAGGCCATGGACAAACACAACCTGGAGGAGGAGGAGCCGGAGGACTA TGAGCTGCTGCAGATTCTCTCAGATGACCGGAAGCTGAAGATCCCTGAAAACGCCAACGTCTTCTATGCC ATGAACTCTACCGCCAACTATGACTTTGTCCTCAAGAAGCGGACCTTCACCAAGGGAGTGAAGGTCAAGC ACGGAGCCAGCTCCACCCTCCCTCGCATGAAGCAGAAAGGACTCAAGATTGCCAAGGGCATCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234993 representing NM_001271774
Red=Cloning site Green=Tags(s) MCLWGHSTAPAHTLSSPPLLFCSLPCALHLQPGTGHPPGQVPRKSSTQEIGEELINGVIYSISLRKVQLH HGGNKGQRWLGYENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVL DLLFKRYGCILPYSDEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLER RAHLLLAQLEHSEPIEAEPEALSPVPALKPTPELELALTPARAPSPVPAPAPEPEPAPTPAPGSELEVAP APAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQPSWPSPVVAENGLSEEK PHLLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVTQFNSVANCVITTCL GNRSTKAPDRARVVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIF SDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGIIQGTVPYLGTFLTDLVMLDTAMKDYLYGRL INFEKRRKEFEVIAQIKLLQSACNNYSIAPDEQFGAWFRAVERLSETESYNLSCELEPPSESASNTLRTK KNTAIVKRWSDRQAPSTELSTSGSSHSKSCDQLRCGPYLSSGDIADALSVHSAGSSSSDVEEINISFVPE SPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVAATRTHKRSVSGLCNSSSALPLYNQQVGDC CIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYA MNSTANYDFVLKKRTFTKGVKVKHGASSTLPRMKQKGLKIAKGIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271774 |
ORF Size | 2655 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271774.1, NP_001258703.1 |
RefSeq Size | 3621 bp |
RefSeq ORF | 2658 bp |
Locus ID | 5900 |
Cytogenetics | 9q34.13-q34.2 |
Protein Pathways | Colorectal cancer, Pancreatic cancer, Pathways in cancer |
MW | 97.8 kDa |
Gene Summary | 'Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]' |
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